Selective BH3 mimetics synergize with BET inhibition to induce mitochondrial apoptosis in rhabdomyosarcoma cells

Autor: Ufuk Erdogdu, Nadezda Dolgikh, Stephanie Laszig, Vinzenz Särchen, Michael T. Meister, Marek Wanior, Stefan Knapp, Cathinka Boedicker

Publikováno v: Neoplasia: An International Journal for Oncology Research, Vol 24, Iss 2, Pp 109-119 (2022)
Neoplasia (New York, N.Y.)
Neoplasia

Highlights • Co-inhibition of BET proteins and anti-apoptotic BCL-2 proteins induces apoptosis in RMS. • JQ1 and BH3-mimetics synergistically induce cell death in RMS. • Cell death is caspase-dependent and displays hallmarks of intrinsic apopto

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8bbac6aac81f4d40d574992bf92415f7
https://doi.org/10.1016/j.neo.2021.11.012

J-domain proteins promote client relay from Hsp70 during tail-anchored membrane protein targeting

Autor: Shu-ou Shan, Yumeng Liu, Hyunju Cho, Woo Jun Shim

Publikováno v: The Journal of Biological Chemistry

J-domain proteins (JDPs) play essential roles in Hsp70 function by assisting Hsp70 in client trapping and regulating the Hsp70 ATPase cycle. Here, we report that JDPs can further enhance the targeting competence of Hsp70-bound client proteins during

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ca9faa735b7e4cad00a3ec62a45a219f
https://resolver.caltech.edu/CaltechAUTHORS:20210317-150527513

A pooled CRISPR/AsCpf1 screen using paired gRNAs to induce genomic deletions in Chinese hamster ovary cells

Autor: Gerald Klanert, Neža Novak, Heena Dhiman, Evgenija Serafimova, Valerie Schmieder, Helene Faustrup Kildegaard, Ly Ngoc Nguyen, Martina Baumann, Nicole Borth

Publikováno v: Schmieder, V, Novak, N, Dhiman, H, Nguyen, L N, Serafimova, E, Klanert, G, Baumann, M, Kildegaard, H F & Borth, N 2021, ' A pooled CRISPR/AsCpf1 screen using paired gRNAs to induce genomic deletions in Chinese hamster ovary cells ', Biotechnology Reports, vol. 31, e00649 . https://doi.org/10.1016/j.btre.2021.e00649
Biotechnology Reports, Vol 31, Iss, Pp e00649-(2021)
Biotechnology Reports

Highlights • Development of a small-scale CRISPR/AsCpf1 screen in CHO. • Usage of paired gRNAs enables full deletion of coding or noncoding genomic regions. • Growth perturbing paired gRNAs identified. • Key points for considerations in futur

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d31d246764716135df37256ce4e0360d
https://orbit.dtu.dk/en/publications/2952ac8e-767c-4605-811b-710e6cd4ba19

Glycosyltransferase ST6Gal-I promotes the epithelial to mesenchymal transition in pancreatic cancer cells

Autor: Michael R. Crowley, Asmi Chakraborty, Donald J. Buchsbaum, Austin D. Silva, Susan L. Bellis, Nikita Bhalerao, Colleen M. Britain, Yvonne J. K. Edwards, David K. Crossman

Publikováno v: The Journal of Biological Chemistry

ST6Gal-I, an enzyme upregulated in numerous malignancies, adds α2-6-linked sialic acids to select membrane receptors, thereby modulating receptor signaling and cell phenotype. In this study, we investigated ST6Gal-I's role in epithelial to mesenchym

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9edcede45860745dd7a00cf70de01157
https://pubmed.ncbi.nlm.nih.gov/33148698

Astrocytic transcription factor REST upregulates glutamate transporter EAAT2, protecting dopaminergic neurons from manganese-induced excitotoxicity

Autor: Michael Aschner, Karam F.A. Soliman, Eun Sook Lee, Deok-Soo Son, Ivan Nyarko-Danquah, Alexis Digman, Edward Alain B. Pajarillo

Publikováno v: The Journal of Biological Chemistry

Chronic exposure to high levels of manganese (Mn) leads to manganism, a neurological disorder with similar symptoms to those inherent to Parkinson's disease. However, the underlying mechanisms of this pathological condition have yet to be established

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bb027f882dbfb30f4fe78b25a2cf0a52
https://doi.org/10.1016/j.jbc.2021.101372