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Autor:
Luca Jovine, Elisa Dioguardi, Isha Raj, Marcel Bokhove, Katharina Gegenschatz-Schmid, K. Nishimura, Hamed Sadat Al Hosseini, Takako Saito, Daniele de Sanctis, Ling Han
Publikováno v:
Journal of Structural Biology
We present a strategy to obtain milligrams of highly post-translationally modified eukaryotic proteins, transiently expressed in mammalian cells as rigid or cleavable fusions with a mammalianized version of bacterial maltose-binding protein (mMBP). T