Zobrazeno 1 - 10
of 3 357
pro vyhledávání: '"EGFP"'
Publikováno v:
Frontiers in Microbiology, Vol 15 (2024)
IntroductionSenecavirus A (SVA), belonging to the genus Senecavirus in the family Picornaviridae, is an emerging pathogen causing vesicular disease in pigs. The main clinical manifestations of SVA infection include high mortality in neonatal piglets,
Externí odkaz:
https://doaj.org/article/fb58f334d87c46e4b9a5412e726c1b56
Autor:
Yingqi Li, Jinjin Duan, You Li, Meihui Zhang, Jiaan Wu, Guanhao Wang, Shuanqing Li, Zhangsen Hu, Yi Qu, Yunhe Li, Xiran Hu, Fei Guo, Lining Cao, Jianfeng Lu
Publikováno v:
Stem Cell Research & Therapy, Vol 15, Iss 1, Pp 1-16 (2024)
Abstract Background The detailed transcriptomic profiles during human serotonin neuron (SN) differentiation remain elusive. The establishment of a reporter system based on SN terminal selector holds promise to produce highly-purified cells with an ea
Externí odkaz:
https://doaj.org/article/06e6e4f9f65a44be8acc6c99549514a4
Publikováno v:
Frontiers in Cell and Developmental Biology, Vol 12 (2024)
Externí odkaz:
https://doaj.org/article/29f0db152f5d4c1987dc7ebe338611e0
Autor:
Van Giap Do, Seonae Kim, Nay Myo Win, Soon-Il Kwon, Hunjoong Kweon, Sangjin Yang, Juhyeon Park, Gyungran Do, Youngsuk Lee
Publikováno v:
Plants, Vol 13, Iss 19, p 2803 (2024)
Genetic transformation is a critical tool for gene manipulation and functional analyses in plants, enabling the exploration of key phenotypes and agronomic traits at the genetic level. While dicotyledonous plants offer various tissues for in vitro cu
Externí odkaz:
https://doaj.org/article/d495d0b4e12645b69430a09ed64373ae
Publikováno v:
Biomolecules, Vol 14, Iss 9, p 1131 (2024)
Introducing genetic material into hard-to-transfect mammalian cell lines and primary cells is often best achieved through retroviral infection. An ideal retroviral vector should offer a compact, selectable, and screenable marker while maximizing tran
Externí odkaz:
https://doaj.org/article/5813c95a2a794d7ebaedb953f0816c93
Autor:
Oleg F. Kandarakov, Natalia S. Polyakova, Alexandra V. Petrovskaya, Alexandra V. Bruter, Alexander V. Belyavsky
Publikováno v:
International Journal of Molecular Sciences, Vol 25, Iss 12, p 6353 (2024)
At present, the magnetic selection of genetically modified cells is mainly performed with surface markers naturally expressed by cells such as CD4, LNGFR (low affinity nerve growth factor receptor), and MHC class I molecule H-2Kk. The disadvantage of
Externí odkaz:
https://doaj.org/article/89cae2b40b644b4691e8e85d70c1015c
Autor:
Daniela Boselli, Maddalena Panigada, Simona Di Terlizzi, Monica Romanò, Emanuele Canonico, Chiara Villa, Claudia Minici, Eelco van Anken, Elisa Soprana, Antonio G. Siccardi
Publikováno v:
Journal of Translational Medicine, Vol 21, Iss 1, Pp 1-14 (2023)
Abstract Background Recombinant MVAs (rMVAs) are widely used both in basic and clinical research. Our previously developed Red-to-Green Gene Swapping Method (RGGSM), a cytometry-based Cell-Sorting protocol, revolves around the transient expression of
Externí odkaz:
https://doaj.org/article/8ed26165057b40fcafc217a4d6c62932
Publikováno v:
Xi'an Gongcheng Daxue xuebao, Vol 37, Iss 3, Pp 51-58 (2023)
To provide regulatory elements for the regulation of metabolic fluxes in Saccharomyces cerevisiae cells and to control the efficient and precise expression of key genes at the transcriptional level in the cells, cloning and characterization studies o
Externí odkaz:
https://doaj.org/article/eb3238612b824eee968173a1742eb64e
Autor:
WANG Jingyi, WANG Qiong
Publikováno v:
Shanghai Jiaotong Daxue xuebao. Yixue ban, Vol 43, Iss 4, Pp 417-427 (2023)
Objective·To establish a T-box transcription factor Brachyury (T gene) fluorescence reporter cell line, in which foot-and-mouth disease virus 2A (F2A) and enhanced green fluorescent protein (EGFP) were knocked in at the end of mouse T gene (mT-F2A-E
Externí odkaz:
https://doaj.org/article/1d4186649e3343baa6e62e94e49b6cd1
Autor:
Nidaa Abu-Rmailah, Liat Moscovici, Carolin Riegraf, Hadas Atias, Sebastian Buchinger, Georg Reifferscheid, Shimshon Belkin
Publikováno v:
Biosensors, Vol 14, Iss 4, p 193 (2024)
The release of endocrine-disrupting compounds (EDCs) to the environment poses a health hazard to both humans and wildlife. EDCs can activate or inhibit endogenous endocrine functions by binding hormone receptors, leading to potentially adverse effect
Externí odkaz:
https://doaj.org/article/5de2886f3a3d4d9797d71602655a0fed