Efficiency Improvements and Discovery of New Substrates for a SARS-CoV-2 Main Protease FRET Assay

Autor: Nikos Kuehl, Mira A. M. Behnam, Christian D. Klein, Tonko Drazic, Mila M. Leuthold

Publikováno v: Slas Discovery

The COVID-19 pandemic, caused by the SARS-CoV-2 virus, has a huge impact on the world. Although several vaccines have recently reached the market, the development of specific antiviral drugs against SARS-CoV-2 is an important additional strategy in f

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f6933ce0cb492634b829401d5c08ed87
https://pubmed.ncbi.nlm.nih.gov/34151620

Polymeric fluorescent heparin as one-step FRET substrate of human heparanase

Autor: Ravi C. Kalathur, Shravan Morla, Umesh R. Desai, Bhaumik B. Patel, Alhumaidi B Alabbas, Chetna Sharon, Jyothi C. Sistla

Publikováno v: Carbohydrate Polymers. 205:385-391

Heparanase, an endo-β-D-glucuronidase, cleaves cell surface and extracellular matrix heparan sulfate (HS) chains and plays important roles in cellular growth and metastasis. Heparanase assays reported to-date are labor intensive, complex and/or expe

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::237fce5bd580f61016a0b6d9fe9010bd
https://doi.org/10.1016/j.carbpol.2018.10.071

Fluorogenic Bifunctional trans-Cyclooctenes as Efficient Tools for Investigating Click-to-Release Kinetics

Autor: Sylvestre Bonnet, Dmitri V. Filippov, Sander I. van Kasteren, Jeroen D. C. Codée, Herman S. Overkleeft, Max S. Kloet, Kiki Kamphorst, Marc S. Robillard, Elmer Maurits, Mark A. R. de Geus, Andrea Pannwitz, Alexi J. C. Sarris, Wolter ten Hoeve, Thomas Hansen

Publikováno v: de Geus, M A R, Maurits, E, Sarris, A J C, Hansen, T, Kloet, M S, Kamphorst, K, ten Hoeve, W, Robillard, M S, Pannwitz, A, Bonnet, S A, Codée, J D C, Filippov, D V, Overkleeft, H S & van Kasteren, S I 2020, ' Fluorogenic Bifunctional trans-Cyclooctenes as Efficient Tools for Investigating Click-to-Release Kinetics ', Chemistry-A European Journal, vol. 26, no. 44, pp. 9900-9904 . https://doi.org/10.1002/chem.201905446
Chemistry-A European Journal, 26(44), 9900-9904. Wiley-VCH Verlag
Chemistry (Weinheim an Der Bergstrasse, Germany)
Chemistry – A European Journal
Chemistry-a European Journal, 26(44), 9900-9904

The inverse electron demand Diels–Alder pyridazine elimination reaction between tetrazines and allylic substituted trans‐cyclooctenes (TCOs) is a key player in bioorthogonal bond cleavage reactions. Determining the rate of elimination of alkylami

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2934b0805fc36e3b284866a7c8515227
https://research.vu.nl/en/publications/c62a99a8-a2c4-41e0-8633-309843ad9529

Development of a peptide substrate for detection of sunn pest damage in wheat flour

Autor: Fahriye Ceyda Dudak, Begüm Zeynep Hançerlioğulları, Hamit Köksel

Publikováno v: Journal of the Science of Food and Agriculture. 98:5677-5682

Since the common protease substrates did not give satisfactory results for the determination of Sunn pest protease activity in damaged wheat, different peptide substrates derived from the repeated sequences of high molecular weight glutenin subunits

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::2e6d91e0e1d23d288a3ebc1dfefae10e
https://doi.org/10.1002/jsfa.9113

Intermolecular quenching of edans/Dabcyl Donor–Acceptor FRET pair

Autor: Susana P. G. Costa, M. Manuela M. Raposo, Cátia D. F. Martins

The intermolecular quenching between 5-(2′-aminoethyl)aminonaphthalene sulfonic acid (Edans) and 4-[[4′-(N,N-dimethylamino)phenyl]diazenyl]benzoic acid (Dabcyl) was studied by photometric and fluorimetric measurements at pH 7.5 in phosphate buffe

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a69d3a9a7a073e0c5479028e5119a397
https://hdl.handle.net/1822/64283