Zobrazeno 1 - 10
of 183
pro vyhledávání: '"E. Sulkowski"'
Publikováno v:
The Journal of Peptide Research. 51:91-95
Alzheimer's amyloid peptide, A beta(1-42) and its fragments, A beta(1-28) and A beta(1-16), were chromatographed on IDA-M(II) columns (M: Cu2+, Ni2+ and Zn2+). The retention of A beta(1-42) and its fragments on IDA-Cu(II) could not be reversed in dec
Publikováno v:
The Journal of Peptide Research. 66:99-105
Alzheimer's disease is characterized by a progressive formation of senile plaques in the brain, the major constituent of which is β-amyloid (Aβ) peptide, a proteolytic product of the transmembrane β-amyloid precursor protein (APP). Prior to the me
Publikováno v:
Bioseparation. 2(1)
The interaction of several serum albumins with chelated (iminodiacetate, IDA) and immobilized (agarose-IDA) metal ions, Co2+, Ni2+, Cu2+ and Zn2+, was studied. There was no retention of human, bovine, porcine, murine and avian albumins on IDA-Zn(II)
Publikováno v:
Biochemistry. 15:5182-5187
Blue Dextran (Cibacron Blue F3GA-dextran) was immobilized on cyanogen bromide activated agarose and used as a ligand for human fibroblast and leukocyte interferons in a solvent of phosphate-buffered (pH 7.4), physiological saline (0.15 M NaCl). Fibro
Publikováno v:
Journal of Biological Chemistry. 253:7612-7615
Human immune interferon, induced in leukocytes by phytohemagglutinin, was prepared in the absence and presence of tunicamycin, an antibiotic which selectively inhibits the glycosylation of newly synthesized glycoproteins. Interferon preparations, pro
Publikováno v:
Biochemical and Biophysical Research Communications. 61:273-281
A four step procedure, including chromatography on Concanavalin-A Sepharose, and leading to a 350 fold purification of exonuclease from venom of Crotalus adamanteus is described. The yield is about 35%. The enzyme is essentially free of 5′-nucleoti
Publikováno v:
Analytical Biochemistry. 38:393-400
Homoribopolymers (A, U, and C) were hydrolyzed with micrococcal nuclease under conditions resulting in maximum yield of 3′-dinucleotides. Dinucleotides were isolated by paper chromatography and free-flow electrophoresis with the following recoverie
Publikováno v:
Journal of Biological Chemistry. 238:2477-2486
Autor:
E. Sulkowski, M. Laskowski
Publikováno v:
Journal of Biological Chemistry. 237:2620-2625
Autor:
E. Sulkowski, M. Laskowski
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis. 240:443-447
A commercial preparation of venom exonuclease (venom phosphodiesterase, VPH, Worthington) was incubated at 37°, pH 3.6 for 3 h. The contaminating 5′-nucleotidase activity was reduced by a factor between 100-and 1000-fold, without a loss of exonucl