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pro vyhledávání: '"E. Regina Valcarcel"'
Publikováno v:
International journal of radiation oncology, biology, physics. 23(5)
To learn more about cellular responses to DNA double-strand breakage, we used three methods to assay cellular damage after treatment with a restriction enzyme that causes DNA double-strand breaks by cleaving at specific recognition sites in the DNA.
Autor:
Richard A. Winegar, Elma Abella Columna, Barbara L. Yates, William F. Morgan, E. Regina Valcarcel
Publikováno v:
Radiation Research. 127:101
Once electroporated into the nucleus of eukaryotic cells, restriction enzymes will bind at specific DNA sequences and cleave DNA to make double-strand breaks. These induced breaks can lead to chromosome aberrations and consequently offer one approach