Výsledky vyhledávání - "E. P. Vrzheshch"

Application of laser fluorimetry for determining the influence of a single amino-acid substitution on the individual photophysical parameters of a fluorescent form of a fluorescent protein mRFP1

Autor: E P Vrzheshch, Evgeny A. Shirshin, Alexandr Banishev

Publikováno v: Quantum Electronics. 39:273-278

Individual photophysical parameters of the chromophore of a fluorescent protein mRFP1 and its two mutants (amino-acid substitution at position 66 – mRFP1/ Q66C and mRFP1/Q66S proteins) are determined. For this purpose, apart from conventional metho

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::f19f1bb8715678d21b15a2b63193e07c
https://doi.org/10.1070/qe2009v039n03abeh013953

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Optical properties of the monomeric red fluorescent protein mRFP1

Autor: A. M. Saletsky, E. P. Vrzheshch, P. V. Vrzheshch, G. S. Rudanov, V. E. Zagidullin, Vladimir Z. Paschenko, D. V. Dmitrienko, A. P. Razzhivin

Publikováno v: Moscow University Biological Sciences Bulletin. 63:109-112

For the expression in E. coli, the PCR-amplified fragment encoding mRFP1 from vector pMT-mRFP1 (Fungal Genetic Stock Center) was placed in the pQE-60 vector. Chemically competent E. coli ER1821 were transformed and grown overnight at 37°C. The prote

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::aaf157539713f70937b4c22a4b3833d5
https://doi.org/10.3103/s0096392508030036

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Mutants of monomeric red fluorescent protein mRFP1 at residue 66: structure modeling by molecular dynamics and search for correlations with spectral properties

Autor: Drutsa Vl, D. V. Dmitrienko, P. V. Vrzheshch, Ekaterina Khrameeva, E. P. Vrzheshch

Publikováno v: Biochemistry. Biokhimiia. 73(10)

To study the interrelation between the spectral and structural properties of fluorescent proteins, structures of mutants of monomeric red fluorescent protein mRFP1 with all possible point mutations of Glu66 (except replacement by Pro) were simulated

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::41c310c43d1fc1a2e77d4dcffef866a3
https://pubmed.ncbi.nlm.nih.gov/18991554

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Red fluorescent protein DsRed: parametrization of its chromophore as an amino acid residue for computer modeling in the OPLS-AA force field

Autor: E. P. Vrzheshch, P. V. Vrzheshch, Drutsa Vl, D. V. Dmitrienko

Publikováno v: Biochemistry. Biokhimiia. 71(10)

Topology of the neutral form of the DsRed fluorescent protein chromophore as a residue of [(4-cis)-2-[(1-cis)-4-amino-4-oxobutanimidoyl]-4-(4-hydroxybenzylidene)-5-oxo-4,5-dihydro-1H-imidazol-1-yl]acetic acid was calculated with OPLS-AA force field.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ddccead48857c0a96aaac5d481905940
https://pubmed.ncbi.nlm.nih.gov/17125463

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