Zobrazeno 1 - 10
of 92
pro vyhledávání: '"E. Neil Lewis"'
Publikováno v:
International Journal of Molecular Sciences, Vol 16, Iss 8, Pp 17719-17733 (2015)
A detailed understanding of the mechanism of unfolding, aggregation, and associated rheological changes is developed in this study for β-Lactoglobulin at different pH values through concomitant measurements utilizing dynamic light scattering (DLS),
Externí odkaz:
https://doaj.org/article/fd6b9a33208443ba9f512b5c9ad41b35
Publikováno v:
Materials, Vol 8, Iss 6, Pp 3754-3766 (2015)
The correlation between molecular changes and microstructural evolution of rheological properties has been demonstrated for the first time in a mixed anionic/zwitterionic surfactant-based wormlike micellar system. Utilizing a novel combination of DLS
Externí odkaz:
https://doaj.org/article/b04a6850dfbf4aa38ec19f171be43def
Publikováno v:
Molecules, Vol 19, Iss 12, Pp 20888-20905 (2014)
Determination of the physicochemical properties of protein therapeutics and their aggregates is critical for developing formulations that enhance product efficacy, stability, safety and manufacturability. Analytical challenges are compounded for mate
Externí odkaz:
https://doaj.org/article/2f2a45e3b4484d2eb27013cb3bf982df
Publikováno v:
Fourier Transform Spectroscopy.
We have developed a new Fourier transform infrared chemical imaging technique1 which, when coupled with powerful multivariate data processing methods, allows the visualization of intrinsic chemical distributions in biological samples and other compos
Autor:
Dean C. Ripple, Karoline Bechtold-Peters, Rukman De Silva, E. Neil Lewis, Anja Matter, Olga Laskina, Linda O. Narhi, Roman Mathaes, Richard E. Cavicchi, John F. Carpenter, Sophia Kenrick, Ellen Koepf, Andrea Hawe, Sambit R. Kar
Publikováno v:
The AAPS journal. 22(1)
The phase-appropriate application of analytical methods to characterize, monitor, and control particles is an important aspect of the development of safe and efficacious biotherapeutics. The AAPS Product Attribute and Biological Consequences (PABC) f
Publikováno v:
Journal of Pharmaceutical Sciences. 105:551-558
To generate aggregates, 3 insulin analogs, lispro, aspart, and glulisine, were incubated without phenolic preservatives for 30 days at 37 °C. As a function of incubation time, aggregation was quantified with size exclusion chromatography, and the si
Publikováno v:
Langmuir. 31:10351-10357
Although critical in applications, the dynamics of colloidal systems at low Péclet numbers is poorly understood. Here we introduce an optical technique that permits for the first time a complete characterization of this regime through a continuous a
Publikováno v:
Analytical Biochemistry. 472:7-20
A Raman spectrometer and dynamic light scattering system were combined in a single platform (Raman–DLS) to provide concomitant higher order structural and hydrodynamic size data for therapeutic proteins at high concentration. As model therapeutic p
Publikováno v:
Molecules
Molecules, Vol 19, Iss 12, Pp 20888-20905 (2014)
Volume 19
Issue 12
Pages 20888-20905
Molecules, Vol 19, Iss 12, Pp 20888-20905 (2014)
Volume 19
Issue 12
Pages 20888-20905
Determination of the physicochemical properties of protein therapeutics and their aggregates is critical for developing formulations that enhance product efficacy, stability, safety and manufacturability. Analytical challenges are compounded for mate
Publikováno v:
Journal of pharmaceutical sciences. 105(8)
The first goal of this study was to determine the effects of the surface fraction of protein in lyophilized formulations of intravenous immunoglobulin on protein stability during long-term storage. We attempted to modulate surface fraction by either