Zobrazeno 1 - 10
of 262
pro vyhledávání: '"E. McGann"'
Publikováno v:
Translational Medicine of Aging, Vol 5, Iss , Pp 31-38 (2021)
Accurate transcription is required for the faithful expression of genetic information. However, little is known about the fidelity of RNA synthesis or the biological consequences of transcription errors in living cells. Here, we review recent develop
Externí odkaz:
https://doaj.org/article/dd8a40c663ed42eebc68cdfd1425e37b
Publikováno v:
PLoS ONE, Vol 16, Iss 4, p e0249814 (2021)
The blood-brain barrier (BBB) keeps pathogens and toxins out of the brain but also impedes the entry of pharmaceuticals. Human cerebral microvascular endothelial cells (hCMECs) and astrocytes are the main functional cell components of the BBB. Althou
Externí odkaz:
https://doaj.org/article/c284bc92652f47328326f72fb298e3fe
Publikováno v:
Translational Medicine of Aging, Vol 5, Iss, Pp 31-38 (2021)
Accurate transcription is required for the faithful expression of genetic information. However, little is known about the fidelity of RNA synthesis or the biological consequences of transcription errors in living cells. Here, we review recent develop
Publikováno v:
The journal of physical chemistry. B. 125(47)
Cryopreservation is the use of very low subzero temperatures to preserve cells and tissues for later use. This is achieved by controlled cooling in the presence of cryoprotectants that moderate the amount of ice formed. Mathematical modeling of the c
Autor:
Leah A. Marquez-Curtis, Xiao-Qing Dai, Yan Hang, Jonathan Y. Lam, James Lyon, Jocelyn E. Manning Fox, Locksley E. McGann, Patrick E. MacDonald, Seung K. Kim, Janet A. W. Elliott
Publikováno v:
PLoS ONE, Vol 17, Iss 1 (2022)
PLoS ONE
PLoS ONE, Vol 17, Iss 1, p e0263005 (2022)
PLoS ONE
PLoS ONE, Vol 17, Iss 1, p e0263005 (2022)
The objective of this study is to optimize the cryopreservation of dissociated islet cells and obtain functional cells that can be used in single-cell transcriptome studies on the pathology and treatment of diabetes. Using an iterative graded freezin
Akademický článek
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Autor:
Locksley E. McGann, Maria Zhurova, Taylor Rutt, Nasim Eskandari, Janet A.W. Elliott, Jason P. Acker, John A. Nychka
Publikováno v:
Cryobiology. 86:134-139
Despite success in cryopreservation of cells in suspension, cryopreservation of cells in monolayers is still challenging. One of the major problems is detachment of the cells from the substrate which occurs during cryopreservation. We hypothesized th
Publikováno v:
Cryobiology. 85:63-72
Cryopreservation of endothelium is one of the major challenges in the cryopreservation of complex tissues. Human umbilical vein endothelial cells (HUVECs) in suspension are available commercially and recently their post-thaw cell membrane integrity w
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2180
One of the major challenges in the preservation of complex tissues is the cryosensitivity of the endothelium, the single layer of cells lining blood vessels, corneas, and other tissues. The increasing importance of endothelial monolayers in tissue-en
Publikováno v:
Cryopreservation and Freeze-Drying Protocols ISBN: 9781071607824
One of the major challenges in the preservation of complex tissues is the cryosensitivity of the endothelium, the single layer of cells lining blood vessels, corneas, and other tissues. The increasing importance of endothelial monolayers in tissue-en
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d1be55467dc146f765bc704770aac882
https://doi.org/10.1007/978-1-0716-0783-1_30
https://doi.org/10.1007/978-1-0716-0783-1_30