Výsledky vyhledávání - "E. López-Urueña"

Effect of colloid (Androcoll-Bear, Percoll, and PureSperm) selection on the freezability of brown bear (Ursus arctos) sperm

Autor: Mercedes Alvarez, S. Borragan, E. López-Urueña, P. de Paz, Luis Anel, Manuel Alvarez-Rodriguez, Jane M. Morrell, L. Anel-López, P. Manrique

Publikováno v: Digital.CSIC. Repositorio Institucional del CSIC
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The development of a species-specific conservation protocol that involves artificial insemination with frozen semen needs to validate an effective methodology for freezing semen. Colloid centrifugation has been suggested and widely applied as an effe

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1bcc74728fa01fb1dc8ccc6682b725f4
https://doi.org/10.1016/j.theriogenology.2015.11.021

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Head morphology of ram spermatozoa is associated with their ability to migrate in vitro and correlates with fertility

Autor: L. Anel-López, E. López-Urueña, Paulino de Paz, Luis Anel, S. Gomes-Alves, Mercedes Alvarez, C. Martínez-Rodríguez, Jorge E. Tizado

Publikováno v: Digital.CSIC. Repositorio Institucional del CSIC
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Fertility is a highly complex biological function that depends on several properties of spermatozoa that are necessary for them to overcome various barriers in the female reproductive tract to reach the fertilisation site. This ability has been evalu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b03079764f2af3b7c94484af3eda6d8b
http://hdl.handle.net/10261/141599

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The use of gelatine in long-term storage (up to 48 hr) at 5°C preserves the pre-freezing and post-thawing quality of brown bear sperm

Autor: Mercedes Alvarez, E. López-Urueña, P. de Paz, Luis Anel, C. Ortega Ferrusola, L. Anel-López, S. Borragan, P. Manrique

Publikováno v: Digital.CSIC. Repositorio Institucional del CSIC
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Sedimentation of spermatozoa occurs during long-term liquid storage and this may produce deleterious changes. Our aim was to apply gelatine supplementation during long-term pre-freezing storage of bear sperm, applying final dilution and 6% glycerol a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6cc3d58e16e40af94c27e1513fd1f9e7
http://hdl.handle.net/10261/141726

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Undiluted or extended storage of ram epididymal spermatozoa as alternatives to refrigerating the whole epididymes

Autor: Mercedes Alvarez, E. Anel, P. de Paz, J. Tamayo-Canul, M. Nicolas, E. López-Urueña, Felipe Martínez-Pastor, Luis Anel

Publikováno v: Animal Reproduction Science. 126:76-82

P. 76-82 The effect of storage procedure at 5 °C on the quality of ram spermatozoa from the cauda epididymis was analyzed. Two strategies were tested at 0, 24, 48 and 72 h post-mortem: (1) spermatozoa held in the epididymal fluid and stored either i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::97395c8ffa08fd975fd9c22e87db7d36
https://doi.org/10.1016/j.anireprosci.2011.04.011

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Optimization of conditions for long-term prefreezing storage of brown bear sperm before cryopreservation

Autor: L. Anel-López, Mercedes Alvarez, S. Borragan, P. Manrique, P. de Paz, E. López-Urueña, C. Martínez-Rodríguez, Luis Anel, S. Gomes-Alves

Publikováno v: Digital.CSIC. Repositorio Institucional del CSIC
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Brown bear ejaculates are usually collected in field conditions and may need to be shipped to a laboratory for the application of reproductive biotechnologies before cryopreservation. The aim of this study was to extend the prefreezing step to 48 hou

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b2b3605e03a7c9c2b913c059c7aaf96a
https://pubmed.ncbi.nlm.nih.gov/26234462

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Refrigerated storage of ram sperm in presence of Trolox and GSH antioxidants: effect of temperature, extender and storage time

Autor: Manuel Alvarez-Rodriguez, J. Tamayo-Canul, Mercedes Alvarez, Felipe Martínez-Pastor, Luis Anel, E. López-Urueña, Paulino de Paz, M. Mata-Campuzano

Publikováno v: Animal reproduction science. 151(3-4)

P. 137-147 Antioxidants have a potential to improve the quality and fertility of refrigerated-stored ram semen. Reduced glutathione (GSH) and Trolox (0.2, 1 and 5 mM) were evaluated in ram semen preserved at 15 and 5 °C up to 48 and 96 h, respective

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5629b2cdbfbc396296142e3ffa13269b
https://pubmed.ncbi.nlm.nih.gov/25458318

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Alternative procedures for the cryopreservation of brown bear ejaculates depending on the flexibility of the >in cooling> period (5°C)

Autor: E. López-Urueña, Luis Anel, S. Borragan, S. Gomes-Alves, Mercedes Alvarez, L. Anel-López, C.A. Chamorro, P. Manrique, P. de Paz

Publikováno v: Digital.CSIC. Repositorio Institucional del CSIC
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The adaptability of cryopreservation protocols for brown bear spermatozoa collected under field conditions and frozen in a nearby laboratory (transported for a few hours) or shipped to a reference laboratory for sex sorting (transported for a few day

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b7445e4a428ba21af29e8e9378bc0f36
http://hdl.handle.net/10261/142315

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Tolerance of brown bear spermatozoa to conditions of pre-freezing cooling rate and equilibration time

Autor: Mercedes Alvarez, Luis Anel, S. Gomes-Alves, S. Borragan, L. Anel-López, P. de Paz, C. Martínez-Rodríguez, E. López-Urueña

Publikováno v: Digital.CSIC. Repositorio Institucional del CSIC
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Specific protocols for the cryopreservation of endangered Cantabrian brown bear spermatozoa are critical to create a genetic resource bank. The aim of this study was to assess the effect of cooling rates and equilibration time before freezing on post

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b389c0f2c49b9cf934d6ecaf14f09816
http://hdl.handle.net/10261/142563

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Ram spermatozoa migrating through artificial mucus in vitro have reduced mitochondrial membrane potential but retain their viability

Autor: Luis Anel, S. Gomes-Alves, L. Anel-López, E. López-Urueña, C. Martínez-Rodríguez, Paulino de Paz, Mercedes Alvarez, C.A. Chamorro

Publikováno v: Reproduction, fertility, and development. 27(5)

Sperm motility in vitro is one of the most common predictors of fertility in male screening. We propose that a mucus-penetration assay can isolate a cellular subpopulation critical to reproductive success. To this end, a device was designed with thre

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cca266c5a69b5cec2ffacf1bfabc912a
https://pubmed.ncbi.nlm.nih.gov/25413445

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Brown bear sperm double freezing: Effect of elapsed time and use of PureSperm® gradient between freeze-thaw cycles

Autor: Luis Anel, L. Anel-López, S. Borragan, Paulino de Paz, Manuel Alvarez-Rodriguez, E. López-Urueña, Mercedes Alvarez, C. Martínez-Rodríguez

Publikováno v: Digital.CSIC. Repositorio Institucional del CSIC
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The use of sexed spermatozoa has great potential to captive population management in endangered wildlife. The problem is that the sex-sorting facility is a long distance from the semen collection place and to overcome this difficulty two freeze-thaw

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::927cb5d7e80425d35f34ea6032d4eb14
http://hdl.handle.net/10261/142662

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