Zobrazeno 1 - 7
of 7
pro vyhledávání: '"E. I. Dement'eva"'
Autor:
E. I. Dement’eva, B. L. Shaskol’skii, A. V. Chestkov, D. A. Gryadunov, Alexey A. Kubanov, Arvo Leinsoo, M. V. Shpilevaya, Dmitry Deryabin, O. A. Obraztsova
Publikováno v:
Bulletin of Experimental Biology and Medicine. 164:54-60
We developed a multiplexed DNA microarray-based assay allowing identification of 12 causative agents of reproductive tract infections with the simultaneous detection of 47 genetic determinants of resistance to antimicrobial substances. The microarray
Autor:
D D, Mamaev, D A, Khodakov, E I, Dement'eva, I V, Filatov, D A, Iurasov, A I, Cherepanov, V A, Vasiliskov, O V, Smoldovskaia, D V, Zimenkov, D A, Griadunov, V M, Mikhaĭlovich, A S, Zasedatelev
Publikováno v:
Prikladnaia biokhimiia i mikrobiologiia. 47(2)
A method and a microfluidic device for automated extraction and purification of nucleic acids from biological samples have been developed. The method involves disruption of bacterial cells and/or viral particles by combining enzymatic and chemical ly
Publikováno v:
Doklady. Biochemistry and biophysics. 381
Publikováno v:
SPIE Proceedings.
We investigated the dynamic properties of two firefly luciferases: Luciola Mingrelica, that contains the only tryptophan residue and Photinus Pyralis, that contains two tryptophan residues by means of fluorescence spectrochronography method. Relaxati
Publikováno v:
Biokhimiia (Moscow, Russia). 61(7)
Recombinant firefly luciferase was expressed in E. coli and its properties inside the intact cells were studied. At low concentrations, antibiotic polymyxin B increases permeability of E. coli cell membrane and concentrations of luciferase substrates
Publikováno v:
Biokhimiia (Moscow, Russia). 61(1)
Physico-chemical properties of the recombinant L. mingrelica luciferase synthesized by E. coli cells have been studied. The catalytic and spectral properties of recombinant luciferase were similar to those of the native enzyme but the former was less
Publikováno v:
Russian Chemical Reviews. 58:1077-1102
The role of genetic engineering in the solution of problems involving enzymology is demonstrated. The method for the cloning and mutagenesis of protein and enzyme genes and the possibilities of genetic engineering for the study of the structure and f