Zobrazeno 1 - 10
of 190
pro vyhledávání: '"E. Howden"'
Autor:
Svenja Pachernegg, Gorjana Robevska, Lucas G.A. Ferreira, Jocelyn A. van den Bergen, Katerina Vlahos, Sara E. Howden, Andrew H. Sinclair, Katie L. Ayers
Publikováno v:
Stem Cell Research, Vol 79, Iss , Pp 103494- (2024)
The transcription factor WT1 plays a critical role in several embryonic developmental processes such as gonadogenesis, nephrogenesis, and cardiac development. We generated a homozygous (MCRIi031-A-3) WT1 knockout induced pluripotent stem cell (iPSC)
Externí odkaz:
https://doaj.org/article/b8430cdb26a34b0fa942cd449540a5ca
Autor:
Svenja Pachernegg, Gorjana Robevska, Lucas G. A. Ferreira, Jocelyn A. van den Bergen, Katerina Vlahos, Sara E. Howden, Andrew H. Sinclair, Katie L. Ayers
Publikováno v:
Stem Cell Research, Vol 79, Iss , Pp 103484- (2024)
The transcription factor SOX9 plays a critical role in several embryonic developmental processes such as gonadogenesis, chrondrogenesis, and cardiac development. We generated heterozygous (MCRIi031-A-1) and homozygous (MCRIi031-A-2) SOX9 knockout ind
Externí odkaz:
https://doaj.org/article/80ae33e8a0664803a7d9924d809260d4
Autor:
Lucas G.A. Ferreira, Mauricio C. Cabral-da-Silva, Svenja Pachernegg, Jocelyn A. van den Bergen, Gorjana Robevska, Katerina Vlahos, Sara E. Howden, Elizabeth S. Ng, Magnus R. Dias-da-Silva, Andrew H. Sinclair, Katie L. Ayers
Publikováno v:
Stem Cell Research, Vol 76, Iss , Pp 103374- (2024)
The NR2F2 gene encodes the transcription factor COUP-TFII, which is upregulated in embryonic mesoderm. Heterozygous variants in NR2F2 cause a spectrum of congenital anomalies including cardiac and gonadal phenotypes. We generated heterozygous (MCRIi0
Externí odkaz:
https://doaj.org/article/65e6a720007544a2b2c11b5ef8415623
Autor:
Peter J. Houweling, Vanessa Crossman, Chrystal F. Tiong, Chantal A. Coles, Rhonda L. Taylor, Joshua S. Clayton, Alison Graham, Katerina Vlahos, Sara E Howden, Kathryn N. North
Publikováno v:
Stem Cell Research, Vol 75, Iss , Pp 103313- (2024)
We used gene editing to introduce DNA sequences encoding the tdTomato fluorescent protein into the α -skeletal actin 1 (ACTA1) locus to develop an ACTA1-tdTomato induced pluripotent stem cell reporter line for monitoring differentiation of skeletal
Externí odkaz:
https://doaj.org/article/75f03b21689441919a85346fd3aa237a
Autor:
Jessica M. Vanslambrouck, Sean B. Wilson, Ker Sin Tan, Ella Groenewegen, Rajeev Rudraraju, Jessica Neil, Kynan T. Lawlor, Sophia Mah, Michelle Scurr, Sara E. Howden, Kanta Subbarao, Melissa H. Little
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-23 (2022)
Abstract While pluripotent stem cell-derived kidney organoids are now being used to model renal disease, the proximal nephron remains immature with limited evidence for key functional solute channels. This may reflect early mispatterning of the nephr
Externí odkaz:
https://doaj.org/article/9f51c78724c14b5daa953e94874378fc
Autor:
Katerina Vlahos, Koula Sourris, Jia Yi Kuah, Alison Graham, Annabelle Suter, Sara E. Howden, Edouard G. Stanley, Andrew G. Elefanty
Publikováno v:
Stem Cell Research, Vol 69, Iss , Pp 103109- (2023)
We describe the generation and characterisation of five human induced pluripotent stem cell (iPSC) lines derived from peripheral blood mononuclear cells (PBMCs) of healthy adult individuals. The PBMCs were reprogrammed using non-integrating Sendai vi
Externí odkaz:
https://doaj.org/article/f127ec15d76c4fbab92c69a6aa7bc4ae
Autor:
Sean B. Wilson, Sara E. Howden, Jessica M. Vanslambrouck, Aude Dorison, Jose Alquicira-Hernandez, Joseph E. Powell, Melissa H. Little
Publikováno v:
Genome Medicine, Vol 14, Iss 1, Pp 1-25 (2022)
Abstract Background While single-cell transcriptional profiling has greatly increased our capacity to interrogate biology, accurate cell classification within and between datasets is a key challenge. This is particularly so in pluripotent stem cell-d
Externí odkaz:
https://doaj.org/article/3cd88183b7f34cd08a2c869067898105
Autor:
Serena Viventi, Stefano Frausin, Sara E. Howden, Shiang Y. Lim, Rocio K. Finol‐Urdaneta, Jeffrey R. McArthur, Kwaku Dad Abu‐Bonsrah, Wayne Ng, Jason Ivanusic, Lachlan Thompson, Mirella Dottori
Publikováno v:
Stem Cells Translational Medicine, Vol 10, Iss 8, Pp 1157-1169 (2021)
Abstract Friedreich ataxia (FRDA) is an autosomal recessive disease characterized by degeneration of dorsal root ganglia (DRG) sensory neurons, which is due to low levels of the mitochondrial protein Frataxin. To explore cell replacement therapies as
Externí odkaz:
https://doaj.org/article/2403739491274763baadfc37b82dc9ee
Autor:
Peter J. Houweling, Chantal A. Coles, Chrystal F. Tiong, Bridget Nielsen, Alison Graham, Penny McDonald, Annabelle Suter, Adam T. Piers, Robin Forbes, Monique M. Ryan, Sara E. Howden, Shireen R. Lamandé, Kathryn N. North
Publikováno v:
Stem Cell Research, Vol 54, Iss , Pp 102429- (2021)
To produce an in vitro model of nemaline myopathy, we reprogrammed the peripheral blood mononuclear cells (PBMCs) of a patient with a heterozygous p.Gly148Asp mutation in exon 3 of the ACTA1 gene to iPSCs. Using CRISPR/Cas9 gene editing we corrected
Externí odkaz:
https://doaj.org/article/fd458bd769a240bdab0c029e7e060663
Autor:
Lorna J. Hale, Sara E. Howden, Belinda Phipson, Andrew Lonsdale, Pei X. Er, Irene Ghobrial, Salman Hosawi, Sean Wilson, Kynan T. Lawlor, Shahnaz Khan, Alicia Oshlack, Catherine Quinlan, Rachel Lennon, Melissa H. Little
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-17 (2018)
Studies examining human podocytopathies have utilised 2D cultured primary or immortalised podocyte cell lines. Here, the authors demonstrate that 3D human glomeruli sieved from induced pluripotent stem cell-derived kidney organoids retain an improved
Externí odkaz:
https://doaj.org/article/1f054c26c4444f92a6c141194b7c9348