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pro vyhledávání: '"E. Eissa Ahmed"'
Autor:
E. Eissa Ahmed
Publikováno v:
International Journal of Horticultural Science, Vol 9, Iss 3-4 (2003)
Callus cultures were induced from hypocotyl of young bean seedlings. Callus developed and maintenaned on B5 medium supplemented with 2mg/1 2,4-D and 1 mg/1 kinetin. The results demonstrate that A. tumefacins-mediated transformation is a convenient me
Externí odkaz:
https://doaj.org/article/d5206021cd5f450891e10bee962eb6bd
Autor:
E. Eissa Ahmed
Publikováno v:
International Journal of Horticultural Science, Vol 9, Iss 3-4 (2003)
Callus cultures were induced from hypocotyl of young bean seedlings. The B5 medium completed with 1 mg/1 KIN and 2mg/1 2,4-D proved the best. Callus developed and maintenaned on B5 medium supplemented with 1mg/1 kinetin and 2mg/I 2,4-D. The B5 medium
Externí odkaz:
https://doaj.org/article/bf8441c232a241369a26cdcdea1bf54f
Publikováno v:
International Journal of Horticultural Science, Vol 7, Iss 1 (2001)
We have found that 50 mg/I kanamycin and 0.8 Mo1/1 mannitol concentration was sufficient to kill the control callus of bean (Phaseolus vulgaris L.) and differentiate transgenic from the non-transgenic cells. The GeneBooster particle delivery system w
Externí odkaz:
https://doaj.org/article/02f81a4203844ed1b6dba6cd131017e5
Publikováno v:
International Journal of Horticultural Science, Vol 6, Iss 4 (2000)
We report the method for the establishment of rapidly growing callus cultures of Phaseolus vulgaris and the conditions required for efficient transformation using high velocity microprojectiles and high level of transient gene expression. Using hypoc
Externí odkaz:
https://doaj.org/article/6d040697a76b49cc9914b008d7fefc68
Publikováno v:
International Journal of Horticultural Science, Vol 6, Iss 1 (2000)
In this paper we report the establishment methods of a rapidly growing callus culture of Phaseolus vulgaris bean as well as the conditions required for a high level of transient gene expression using Agrobacterium-mediated transformation. A vector is
Externí odkaz:
https://doaj.org/article/71541118dcc24a11beeb4a31bac95534
Publikováno v:
International Journal of Horticultural Science, Vol 5, Iss 1-2 (1999)
Dry seeds from two cultivars of common bean (Phaseolus vulgaris L.) were germinated on sterile cotton and sterile deionized distilled water. Cotyledonary node tissue of seedlings were cultured on Murashige and Skoog(MS)-based media supplemented with
Externí odkaz:
https://doaj.org/article/a54665eccca941a598b3fe4fd953471d
Publikováno v:
Acta Horticulturae. :129-132
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