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Akademický článek

Transesterification of phospholipids or triglycerides to fatty acid benzyl esters with simultaneous methylation of free fatty acids for gas-liquid chromatographic analysis.

Autor: F J van Kuijk, D W Thomas, J P Konopelski, E A Dratz

Publikováno v: Journal of Lipid Research, Vol 27, Iss 4, Pp 452-456 (1988)

A novel method is presented for transesterification of fatty acid esters in phospholipids and triglycerides to benzyl esters while simultaneously recovering free fatty acids as methyl esters. Transesterification is catalyzed by 0.2 M (m-trifluorometh

Externí odkaz: https://doaj.org/article/d75e57cd46e14740ba56c609a2262985

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Enrichment of bacteriorhodopsin with isotopically labeled amino acids by biosynthetic incorporation in Halobacterium halobium

Autor: S. L. Helgerson, E. A. Dratz, S. L. Siemsen

Publikováno v: Canadian Journal of Microbiology. 38:1181-1185

The growth of Halobacterium halobium was optimized in a chemically defined synthetic medium. Arginine, isoleucine, leucine, lysine, methionine, tyrosine, and valine were found to be essential for growth. Optimal growth rates and cell yields were obta

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::3eb55500fe84d46a877900d67c8f948c
https://doi.org/10.1139/m92-193

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Antibody imprint of a membrane protein surface. Phagocyte flavocytochrome b

Autor: J B, Burritt, S C, Busse, D, Gizachew, D W, Siemsen, M T, Quinn, C W, Bond, E A, Dratz, A J, Jesaitis

Publikováno v: The Journal of biological chemistry. 273(38)

Structural features of the integral membrane protein flavocytochrome b (Cyt b) were discovered using an antibody "imprint" of the Cyt b surface. Amino acid sequences were selected from a random nonapeptide phage-display library by their affinity for

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::f2eafa6a4d3b7f70acbf2a7499bb3b28
https://pubmed.ncbi.nlm.nih.gov/9733789

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The ligand binding site of the formyl peptide receptor maps in the transmembrane region

Autor: H M, Miettinen, J S, Mills, J M, Gripentrog, E A, Dratz, B L, Granger, A J, Jesaitis

Publikováno v: Journal of immunology (Baltimore, Md. : 1950). 159(8)

We propose that the N-formyl-Met-Leu-Phe binding site in the human neutrophil formyl peptide receptor (FPR) lies in the predicted transmembrane region. We examined the expression, binding, and G protein coupling of 28 mutated forms of FPR in stably t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::68d549afb3cbb2ed37f33ee7e6081a32
https://pubmed.ncbi.nlm.nih.gov/9378994

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Identification of transmembrane tryptic peptides of rhodopsin using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Autor: D R, Barnidge, E A, Dratz, J, Sunner, A J, Jesaitis

Publikováno v: Protein science : a publication of the Protein Society. 6(4)

The application of mass spectrometry for determining the topography of integral membrane proteins has focused primarily on the mass determination of fragments that do not reside in the lipid bilayer. In this work, we present the accurate mass determi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::97189e397f5f0d396c7c84fa57966233
https://pubmed.ncbi.nlm.nih.gov/9098891

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NMR constrained solution structures for laminin peptide 11. Analogs define structural requirements for inhibition of tumor cell invasion of basement membrane matrix

Autor: G J, Ostheimer, J R, Starkey, C G, Lambert, S L, Helgerson, E A, Dratz

Publikováno v: The Journal of biological chemistry. 267(35)

Peptide 11, CDPGYIGSR-NH2, is a segment of laminin which blocks tumor cell invasion. A high affinity laminin receptor in tumor cells is thought to be blocked by the carboxyl-terminal YIGSR, and conformational energy calculations suggest that the glyc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::c14d548a9cc5cf6192419602ae2bb3fa
https://pubmed.ncbi.nlm.nih.gov/1460013

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[19] Measurement of carotenoids in human and monkey retinas

Autor: M. D. Russett, A. J. Adler, E. A. Dratz, D. M. Snodderly, Garry J. Handelman

Publisher Summary This chapter discusses the dissection method of measurement of carotenoids in human and monkey retinas. Proficiency with the dissection method requires practice and familiarity with the structure of the eyeball. The method takes app

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::e78ba9674d3e62b7f05f0b5b05e2a4e0
https://doi.org/10.1016/0076-6879(92)13123-f

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