Zobrazeno 1 - 10
of 521
pro vyhledávání: '"E Vickers"'
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-14 (2024)
Abstract The methylerythritol phosphate (MEP) pathway is responsible for biosynthesis of the precursors of isoprenoid compounds in eubacteria and plastids. It is a metabolic alternative to the well-known mevalonate pathway for isoprenoid production f
Externí odkaz:
https://doaj.org/article/bd68c10359be4db5a7197782ec6374a3
Autor:
Li Chen Cheah, Lian Liu, Manuel R. Plan, Bingyin Peng, Zeyu Lu, Gerhard Schenk, Claudia E. Vickers, Frank Sainsbury
Publikováno v:
Advanced Science, Vol 10, Iss 32, Pp n/a-n/a (2023)
Abstract Enzyme spatial organization is an evolved mechanism for facilitating multi‐step biocatalysis and can play an important role in the regulation of promiscuous enzymes. The latter function suggests that artificial spatial organization can be
Externí odkaz:
https://doaj.org/article/721bd03ae2184538bc1551e2fb53851e
Autor:
Zeyu Lu, Qianyi Shen, Lian Liu, Gert Talbo, Robert Speight, Matt Trau, Geoff Dumsday, Christopher B. Howard, Claudia E. Vickers, Bingyin Peng
Publikováno v:
Engineering Microbiology, Vol 3, Iss 3, Pp 100079- (2023)
Hexokinase II (Hxk2) is a master protein in glucose-mediated transcriptional repression signaling pathway. Degrading Hxk2 through an auxin-inducible protein degradation previously doubled sesquiterpene (nerolidol) production at gram-per-liter levels
Externí odkaz:
https://doaj.org/article/d10c08e372954aa98f98d6f138311977
Autor:
Bingyin Peng, Lygie Esquirol, Zeyu Lu, Qianyi Shen, Li Chen Cheah, Christopher B. Howard, Colin Scott, Matt Trau, Geoff Dumsday, Claudia E. Vickers
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-12 (2022)
Gene dosage-based expression upregulation suffers from instability and random gene integration. Here, the authors report HapAmp, a method that uses haploinsufficiency as evolutionary force to drive in vivo gene amplification, and demonstrate its appl
Externí odkaz:
https://doaj.org/article/e833a815673c4afdbb238b2a86a2e193
Autor:
Bingyin Peng, Naga Chandra Bandari, Zeyu Lu, Christopher B. Howard, Colin Scott, Matt Trau, Geoff Dumsday, Claudia E. Vickers
Publikováno v:
Communications Biology, Vol 5, Iss 1, Pp 1-13 (2022)
Eukaryotic trans-regulatory mechanisms are exploited to synthesize a stringent tetracycline-repressible circuit and a heat-inducible circuit for negative/positive metabolic controls using the GAL system in Saccharomyces cerevisiae.
Externí odkaz:
https://doaj.org/article/f8281b48a1eb4e1c820fe3e041bd5054
Autor:
Claudia E. Vickers, Paul S. Freemont
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-4 (2022)
Synthetic biology has played a key role in responding to the current pandemic. Biofoundries are critical synthetic biology infrastructure which should be available to all nations as a part of their independent bioengineering, biosecurity, and counter
Externí odkaz:
https://doaj.org/article/6f69d81c51e840d8a14cbbb0c7be5802
Autor:
Irfan Farabi Hayat, Manuel Plan, Birgitta E. Ebert, Geoff Dumsday, Claudia E. Vickers, Bingyin Peng
Publikováno v:
Microbial Biotechnology, Vol 14, Iss 6, Pp 2627-2642 (2021)
Summary The yeast Saccharomyces cerevisiae uses the pyruvate dehydrogenase‐bypass for acetyl‐CoA biosynthesis. This relatively inefficient pathway limits production potential for acetyl‐CoA‐derived biochemical due to carbon loss and the cost
Externí odkaz:
https://doaj.org/article/912b849ae4dd4eeab30401b22a5f7992
Autor:
Michelle G. Brown, John F. Lindo, Ivan E. Vickers, Kereann Nelson, Yakima Phillips, Cameil Wilson-Clarke, Samuel Gavi, Gene D. Morse, Andrew H. Talal
Publikováno v:
Tropical Medicine and Infectious Disease, Vol 8, Iss 7, p 370 (2023)
Hepatitis C (HCV) continues to present a global public health challenge, with no vaccine available for prevention. Despite the availability of direct-acting antivirals (DAAs) to cure HCV, it remains prevalent in many regions including the Caribbean.
Externí odkaz:
https://doaj.org/article/5c740c8af7244b4fa4262f4914637822
Publikováno v:
Microbial Biotechnology, Vol 14, Iss 6, Pp 2254-2256 (2021)
Externí odkaz:
https://doaj.org/article/7c4f805fca2e436e89c9a852800ab187
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-13 (2021)
Loss-of-function experiments are used in metabolic engineering to understand and optimise metabolism. Here, the authors apply auxin inducible protein degradation to test different metabolic engineering strategies for improved terpenoid production in
Externí odkaz:
https://doaj.org/article/ec9f52fb74584aa2b989e3d0bc618cc7