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pro vyhledávání: '"E O, Puchkov"'
Autor:
E O Puchkov
Publikováno v:
World Journal of Microbiology and Biotechnology. 37
The upgrading of fluorescence microscopy by the introduction of computer technologies has led to the creation of a new methodology, computerized fluorescence microscopy (CFM). CFM improves subjective visualization and combines it with objective quant
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7af98f7000d1dfdf2868253c8e1a3796
https://doi.org/10.1007/s11274-021-03159-3
https://doi.org/10.1007/s11274-021-03159-3
Autor:
E O, Puchkov
Publikováno v:
World journal of microbiologybiotechnology. 37(11)
The upgrading of fluorescence microscopy by the introduction of computer technologies has led to the creation of a new methodology, computerized fluorescence microscopy (CFM). CFM improves subjective visualization and combines it with objective quant
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::6a680cdbcbdc7885da932ff6d9369255
https://pubmed.ncbi.nlm.nih.gov/34617135
https://pubmed.ncbi.nlm.nih.gov/34617135
Autor:
E. O. Puchkov
Publikováno v:
Microbiology. 88:1-14
—Modern ideas about the world of microorganisms have been developed mainly due to the studies at the population level. Such an approach was justified by the need to overcome the limitations in the sensitivity of the physicochemical, molecular biolo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d664365c2cb8bf20b0852a389226adf6
https://doi.org/10.1134/s0026261719010120
https://doi.org/10.1134/s0026261719010120
Autor:
E. O. Puchkov
Publikováno v:
Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology. 10:1-10
In the microbial world, there are various communicative interactions via secreted chemical intermediates. Some of these interactions can be considered as signaling, as they trigger specific physiological responses by modulating transcription of cogna
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f3ec74a11db643b46365d58ef0fbe1ef
https://doi.org/10.1134/s1990747815050104
https://doi.org/10.1134/s1990747815050104
Autor:
E. O. Puchkov
Publikováno v:
Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology. 7:270-279
This review is devoted to the study of intracellular viscosity. Methods of intracellular viscosity measurement in cell populations and single cells are characterized and critically evaluated. Examples of intracellular viscosity assessment in a number
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::71c3b57ff622cd8e1bf8be7d7de55d1c
https://doi.org/10.1134/s1990747813050140
https://doi.org/10.1134/s1990747813050140
Autor:
M. McCarren, E. O. Puchkov
Publikováno v:
Biophysics. 56:651-655
The intracellular location of nucleic acid intercalators (NAI) in native (not fixed) Saccharomyces cerevisiae cells has been studied using fluorescence microscopy combined with computer pseudospectral image analysis. Three NAI: anthracycline anticanc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9779502b9d6384385721a79d203fb4e2
https://doi.org/10.1134/s0006350911040233
https://doi.org/10.1134/s0006350911040233
Autor:
E. O. Puchkov
Publikováno v:
Microbiology. 79:141-146
This review provides a concise description of the basic principles and options of computer-based image analysis for counting and characterization of microbial colonies. Properties of several modern commercially available computer/automated colony cou
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::abb2a98246ebddbd3282c119a9942d36
https://doi.org/10.1134/s0026261710020025
https://doi.org/10.1134/s0026261710020025
Autor:
E. O. Puchkov
Publikováno v:
Microbiology. 78:502-509
Application of an automated colony counter for evaluation of the viability of microbial cultures was investigated with yeast cultures as a model. Statistical comparison of the results of automated and visual (“manual”) colony counting is presente
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c94f7974eb2334c2155417960203c1e1
https://doi.org/10.1134/s0026261709040158
https://doi.org/10.1134/s0026261709040158
Autor:
E O, Puchkov
Publikováno v:
Prikladnaia biokhimiia i mikrobiologiia. 51(2)
Computerized optical image analysis is a variant of using computerized analysis of images of study objects recorded by optical methods. This review covers the literature and our own data on computer analysis of optical images of microbial origin. It
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::c502d5a835b0a68828c1b678d1ce0faf
https://pubmed.ncbi.nlm.nih.gov/26027365
https://pubmed.ncbi.nlm.nih.gov/26027365
Autor:
E. O. Puchkov
Publikováno v:
Microbiology. 75:154-160
A vital staining of the ethanol-producing yeast Saccharomyces cerevisiae with ethidium bromide and DAPI allows intact and damaged cells to be differentiated by fluorescence microscopy. A computer image analysis procedure is developed for the automati
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b9f82a0c7467eff4f0bf9a677d61b810
https://doi.org/10.1134/s0026261706020081
https://doi.org/10.1134/s0026261706020081