Zobrazeno 1 - 5
of 5
pro vyhledávání: '"E M Slorach"'
Autor:
Anthony J. Brookes, Diane Lawson, E M Slorach, John C. Maule, David J. Porteous, J.E.V. Watson
Publikováno v:
Genome Research. 5:444-452
We describe a strategy to introduce preferentially the dominant selectable marker neoR into the human chromosome within a monochromosome hybrid cell line. Integration of a construct containing the marker is mediated by human-specific repeat elements
Autor:
Anthony J. Brookes, Walter J. Muir, M.L. Thomson, C.M. Gosden, David J. Porteous, Kathryn L. Evans, E M Slorach
Publikováno v:
Mammalian genome : official journal of the International Mammalian Genome Society. 6(4)
An improved protocol has been developed for physical enrichment of cDNA sequences by hybridization to genomic DNA. When applied to microdissection recombinants derived from a translocation breakpoint region associated with inherited mental illness, a
Autor:
R S Devon, E M Slorach, John C. Maule, Y Shibasaki, J R Gosden, David J. Porteous, Anthony J. Brookes
Publikováno v:
PCR methods and applications. 4(4)
A simple and efficient method to generate hapten-labeled DNA fragments from a trace amount of YAC DNA isolated by PFGE is described. After agarase digestion of the gel slice containing the resolved YAC recombinant, the purified DNA is digested with S
Autor:
J. M. Fletcher, Kathryn L. Evans, David J. Porteous, M H Polymeropoulos, E M Slorach, A Seawright, Anthony J. Brookes
Publikováno v:
Cytogenetics and cell genetics. 70(1-2)
Expressed sequence tags (ESTs) provide an efficient route to the identification of genes involved in normal development and in disease. PCR amplification of somatic cell hybrid DNAs was used to localise 22 brain-derived ESTs to subregions of human ch
Publikováno v:
Trends in Genetics. 10:345