Zobrazeno 1 - 10 of 39 pro vyhledávání: '"E M Bailyes"'
2
Endoplasmic reticulum Ca2+ is important for the proteolytic processing and intracellular transport of proinsulin in the pancreatic β-cell
Autor: John C. Hutton, Paul C. Guest, E M Bailyes
Publikováno v: Biochemical Journal. 323:445-450
The role of intracellular Ca2+ in the proteolytic processing and intracellular transport of secretory granule proproteins was investigated by pulse–chase radiolabelling of isolated rat islets of Langerhans. The conversion of proinsulin was inhibite
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::14391d720bb9460fa2d2ac155e74d3cc
https://doi.org/10.1042/bj3230445
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3
HLA-DR-restricted T cell lines from newly diagnosed type 1 diabetic patients specific for insulinoma and normal islet beta cell proteins: lack of reactivity to glutamic acid decarboxylase
Autor: J.M. Tremble, E. M. Bailyes, G. C. Huang, A. M. McGREGOR, J. P. Banga, S. D. Arden, T. Kaye
Publikováno v: Clinical and Experimental Immunology. 102:152-158
SUMMARY T cells reacting with pancreatic islet beta cell proteins play a pivotal role in the pathogenesis of type I diabetes in experimental animal models and man, although the islet cell autoantigens against which these T cells are directed remain t
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3ce40a0157bce72476013a50c50bbff5
https://doi.org/10.1111/j.1365-2249.1995.tb06649.x
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4
Differences between the catalytic properties of recombinant human PC2 and endogenous rat PC2
Autor: Kevin Docherty, Kathleen I.J. Shennan, John C. Hutton, E. F. Usac, E M Bailyes, Paul C. Guest, Susan D. Arden
Publikováno v: Biochemical Journal. 309:587-594
Human prohormone convertase PC2 was expressed in Xenopus oocytes and its properties were compared with those of the Type-2 endopeptidase of rat insulin secretory granules, previously identified as PC2 [Bennett, Bailyes, Nielson, Guest, Rutherford, Ar
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a595b5a586bc82a844da01979e85b60b
https://doi.org/10.1042/bj3090587
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5
Identification of the Region within the Neuroendocrine Polypeptide 7B2 Responsible for the Inhibition of Prohormone Convertase PC2
Autor: W. H. Van Den Hurk, Iris Lindberg, E M Bailyes, Gerard J.M. Martens, A. M. Van Horssen, John C. Hutton
Publikováno v: Journal of Biological Chemistry. 270:14292-14296
The highly conserved polypeptide 7B2 and the subtilisin-related prohormone convertases PC1/PC3 and PC2 are broadly distributed in neurons and endocrine cells and are localized to secretory granules. We recently showed that recombinant 7B2 is in vitro
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ff11098864424a6ce7acca95c8b952b8
https://doi.org/10.1074/jbc.270.24.14292
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7
Kinetic analysis of the type-1 proinsulin endopeptidase by a monoclonal antibody-based immunoadsorbent assay
Autor: E M Bailyes, John C. Hutton
Publikováno v: Biochemical Journal. 286:223-229
A simple, rapid and sensitive assay for the type-1 endopeptidase (Arg-Arg cleaving) was developed by using an antiproinsulin monoclonal immunoadsorbent to separate reaction products from the substrate. The values obtained by this assay were identical
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a89cbae47716920c5ebdbb07034a4d41
https://doi.org/10.1042/bj2860223
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8
Identification of the type 2 proinsulin processing endopeptidase as PC2, a member of the eukaryote subtilisin family
Autor: John C. Hutton, Nicholas G. Rutherford, Deborah L. Bennett, E M Bailyes, E Nielsen, Susan D. Arden, Paul C. Guest
Publikováno v: Journal of Biological Chemistry. 267:15229-15236
Enzymological studies have implicated two Ca(2+)-dependent endopeptidases in the conversion of proinsulin to insulin; a type 1 activity which cleaves on the C-terminal side of Arg31-Arg32 and a type 2 activity which cleaves C-terminally to Lys64-Arg6
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::046bd9b251c2ab7286da2e6cc4b6c191
https://doi.org/10.1016/s0021-9258(18)42170-9
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9
The presence and measurement of secretory component in human bile and blood
Autor: Barbara M. Mullock, E M Bailyes, J H Perez, Christopher P. Price, J. Paul Luzio, Margriet Van Schaik
Publikováno v: Clinica Chimica Acta. 197:171-187
Five monoclonal antibodies which recognized three separate epitopes on the free secretory component molecule were produced using free secretory component obtained from human colostrum. Two-site immunoradiometric assays were developed to measure free
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5f6f0b91485cb2c11cbcb1b42dd544c1
https://doi.org/10.1016/0009-8981(91)90138-3
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10
Insulin secretory granule biogenesis. Co-ordinate regulation of the biosynthesis of the majority of constituent proteins
Autor: Paul C. Guest, Nicholas G. Rutherford, John C. Hutton, E M Bailyes
Publikováno v: Biochemical Journal. 274:73-78
Two-dimensional gel-electrophoretic analysis combined with fluorography and densitometric quantification was used to examine the effects of glucose on the biosynthesis of rat pancreatic islet proteins. An increase in the medium glucose concentration
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a0259de39dc25d088b69450058ec621c
https://doi.org/10.1042/bj2740073
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