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Host-inhibitory functions encoded by promiscuous plasmids. Transient arrest of Escherichia coli segregants that fail to inherit plasmid RK2

Autor: O S, Jovanovic, E K, Ayres, D H, Figurski

Publikováno v: Journal of molecular biology. 237(1)

An effective strategy for a plasmid to be maintained in a growing population of bacteria is to prevent the proliferation of newly formed daughter cells that lack the plasmid. In this study, we determined that the promiscuous, incompatibility group P

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::ff811608cbb9e5f906500b99f46c3053
https://pubmed.ncbi.nlm.nih.gov/8133520

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Precise deletions in large bacterial genomes by vector-mediated excision (VEX). The trfA gene of promiscuous plasmid RK2 is essential for replication in several gram-negative hosts

Autor: E K, Ayres, V J, Thomson, G, Merino, D, Balderes, D H, Figurski

Publikováno v: Journal of molecular biology. 230(1)

We have developed a simple and efficient method of vector-mediated excision (VEX) for in vivo generation of precisely defined deletions in large bacterial genomes. The system uses homologous recombination with small cloned fragments on specialized pV

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::c7a9aeb989ef66fb8c603961fda82f4e
https://pubmed.ncbi.nlm.nih.gov/8450534

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