Zobrazeno 1 - 10
of 14
pro vyhledávání: '"E J Murgola"'
Autor:
A L, Arkov, E J, Murgola
Publikováno v:
Biochemistry. Biokhimiia. 64(12)
It is now well established that ribosomal RNAs (rRNAs) play an active role in every aspect of translation. This review focuses on recent evidence for the involvement of rRNAs from both subunits of the ribosome in translation termination. This evidenc
Publikováno v:
Nucleic acids symposium series. (33)
A single nucleotide change, G to A, at nucleotide position 1093 of E. coli 23S ribosomal RNA was found to cause UGA-specific suppression (D.K. Jemiolo, F.T. Pagel and E.J. Murgola, Proc. Natl. Acad. Sci. USA, in press). To obtain new kinds of UGA-spe
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America. 88(15)
Site-directed mutagenesis was performed on a sequence motif within the 3' major domain of Escherichia coli 16S rRNA shown previously to be important for peptide chain termination. Analysis of stop codon suppression by the various mutants showed an ex
Autor:
J E Bryant, E J Murgola
Publikováno v:
Journal of Bacteriology. 142:131-137
In this paper we describe the successful isolation of glyT-derived GAA suppressors. A glyT+ strain containing glyV55, the gene for a GGA/G-reading, methane sulfonate and hydroxylamine. The cells were plated to select for reversal of auxotrophy due to
Publikováno v:
Journal of Bacteriology. 134:801-807
Glutamic acid codon suppressors in 18 isogenic strains of Escherichia coli have been further characterized as to map location, dominance, growth rates in various media, suppression of the GAG codon, and tRNA profiles after reversed-phase column chrom
Autor:
Edward A. Adelberg, E. J. Murgola
Publikováno v:
Journal of Bacteriology. 103:20-26
Forty-one mutants have been isolated which require streptomycin for growth on complete medium. These streptomycin-suppressible lethal mutations are located randomly around the Escherichia coli genetic map; during growth in liquid culture, they exhibi
Autor:
E J Murgola, F T Pagel
Publikováno v:
Journal of Bacteriology. 156:917-919
We describe a novel class of missense suppressors that read the codons for lysine at two positions (211 and 234) in the trpA polypeptide of Escherichia coli. The suppressor mutations are highly linked to lysT, a gene for lysine tRNA. The results sugg
Publikováno v:
Journal of Bacteriology. 162:837-839
An alanine tRNA with the anticodon 5'-GGC-3' has been identified in Escherichia coli K-12. It is the first sequenced alanine tRNA with G in the 5' position of the anticodon. tRNAAlaGGC has A in the "semi-invariant" position 32. At the "invariant" pos
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America. 85(12)
We have isolated an unusual codon-specific translational suppressor in Escherichia coli. The suppressor resulted from a spontaneous mutation in a chromosomal gene during a selection for suppressors of the auxotrophic nonsense mutation trpA(UGA211). T
Publikováno v:
Journal of bacteriology. 152(2)
By the use of [5'-32P]tRNA3Gly from Escherichia coli as a hybridization probe, glyW was located on cloned fragments of the uvrC pgsA region of the bacterial chromosome. After determination of the sites of action of several restriction enzymes, glyW w