Výsledky vyhledávání - "E I Zvaritch"

Expression, purification, and properties of the plasma membrane Ca2+ pump and of its N-terminally truncated 105-kDa fragment

Autor: Tomoko Iwata, Emanuel E. Strehler, H P Adamo, E I Zvaritch, Ernesto Carafoli, Danilo Guerini, B Rutishauser, Roger Heim

Publikováno v: Scopus-Elsevier

Isoform 4b of the human plasma membrane Ca2+ pump was expressed in COS cells and in the baculovirus system (Sf9 cells). A 105-kDa pump fragment lacking the first two transmembrane domains and the so-called transduction domain was also expressed. The

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d5ad83c735da83b46b3df0f6a9ade93d
https://doi.org/10.1016/s0021-9258(18)35791-0

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Mapping of functional domains in the plasma membrane calcium pump using trypsin proteolysis

Autor: Rocco Falchetto, Thomas Vorherr, Ernesto Carafoli, E I Zvaritch, N N Modyanov, Peter James

Publikováno v: Biochemistry. 29:8070-8076

The purified erythrocyte Ca2+ pump has been exposed to trypsin under conditions designed to enrich the fragments of molecular mass 90, 85, 81, and 76 kDa, respectively. In SDS-polyacrylamide gels, these fragments are accompanied by a product of molec

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::0e2e12c9c64443ee7c0d61a91439d055
https://doi.org/10.1021/bi00487a012

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Elucidation of conservative elements of calmodulin-dependent enzymes with the use of monoclonal antibodies

Autor: Alakhov VYu, N N, Modyanov, M I, Shakhparonov, E I, Zvaritch, M S, Feschenko, S V, Lutzenko

Publikováno v: Biotechnology and applied biochemistry. 10(4)

Monoclonal antibodies against human erythrocyte membrane Ca2+-ATPase were obtained. The binding of monoclonal antibodies to the enzyme resulted in a decrease in the enzyme sensitivity to calmodulin (CaM). The effects of monoclonal antibodies on other

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::cdfc896a9ae0e414cba47dacc4cbd81b
https://pubmed.ncbi.nlm.nih.gov/2464354

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A monoclonal antibody recognizes an epitope in the first extracellular loop of the plasma membrane Ca2+ pump

Autor: N N Modyanov, E I Zvaritch, Mikhail I. Shakhparonov, Ernesto Carafoli, M S Feschenko, Thomas Vorherr, F Hofmann

Publikováno v: Scopus-Elsevier

A monoclonal antibody against the human erythrocyte Ca2+ pump (1E4) reacted with the enzyme in intact erythrocytes. Using trypsinized preparations of the pump the antibody only reacted with the N-terminal fragments of 33.5 and 35 kDa. The fragments s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0760902de85edf42e78e5cd57958cf82
http://www.scopus.com/inward/record.url?eid=2-s2.0-0026671192&partnerID=MN8TOARS

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