Zobrazeno 1 - 10
of 177
pro vyhledávání: '"E Homsher"'
Publikováno v:
American Journal of Physiology-Cell Physiology. 269:C1532-C1539
The regulation of cross-bridge transition from weakly attached to force-bearing states was studied at 10 degrees C in skinned muscle fibers by measuring the rate of force development after a quick release-restretch cycle (ktr), the rate of force decl
Autor:
E. Homsher, N. C. Millar
Publikováno v:
American Journal of Physiology-Cell Physiology. 262:C1239-C1245
The kinetics of the force generating and Pi release steps of the actomyosin-adenosinetriphosphatase (ATPase) cycle have been compared in Ca(2+)-activated skinned fibers of rabbit soleus (slow twitch) and psoas (fast twitch) muscle. Pi was rapidly pho
Publikováno v:
American Journal of Physiology-Cell Physiology. 262:C714-C723
The measurement of fluorescent-labeled actin filament movement driven by mechanoenzymes (e.g., myosin) is an important methodology for the study of molecular motors. It is assumed that the filament velocity (Vf) is analogous to the unloaded shortenin
Publikováno v:
The Journal of biological chemistry. 274(25)
According to the Lorenz et al. (Lorenz, M., Poole, K. J., Popp, D., Rosenbaum, G., and Holmes, K. C. (1995) J. Mol. Biol. 246, 108-119) atomic model of the actin-tropomyosin complex, actin residue Asp-311 (Glu-311 in yeast) is predicted to have a hig
Publikováno v:
Methods in cell biology. 39
Publikováno v:
Advances in experimental medicine and biology. 332
The measurement of fluorescently labeled actin filament movement driven by mechanoenzymes (e.g. myosin) is an important methodology for the study of molecular motors. It is assumed that the filament velocity, Vf, is analogous to the unloaded shorteni
Autor:
E, Homsher, N, Millar
Publikováno v:
Advances in experimental medicine and biology. 332
Recent studies have shown that the release of Pi from the AM.ADP.Pi crossbridge occurs in two steps: an initial force-generating isomerization which is followed by the release per se of Pi. These two steps have been shown to be little affected by the
Publikováno v:
The Journal of physiology. 451
1. Orthophosphate (P(i), 0.1-2.0 mM) was photogenerated within the filament lattice of isometrically contracting glycerinated fibres of rabbit psoas muscle at 10 and 20 degrees C. The P(i) was produced by laser flash photolysis of the photolabile com
Autor:
N C, Millar, E, Homsher
Publikováno v:
The Journal of biological chemistry. 265(33)
The effect of varying concentrations of Pi and Ca2+ on isometric force and on the rate of force development in skinned rabbit psoas muscle fibers has been investigated. Steady-state results show that the three parameters that define the force-pCa rel
Autor:
N C Millar, E Homsher
Publikováno v:
Annual review of physiology. 52
This review summarizes recent work using caged compounds for the study of muscular contraction. We focus on what flash photolysis has taught us about the reaction mechanism and regulation of the actomyosin system of the intact fiber lattice, both dur