Zobrazeno 1 - 10
of 22
pro vyhledávání: '"E G, Sadykhov"'
Publikováno v:
Applied Biochemistry and Microbiology. 57:297-302
A producer strain of Komagataella phaffii of recombinant beluga whale (Delphinapterus leucas) chymosin with a productivity of 80 mg/L was obtained, and the physico-chemical properties of the enzyme were studied for possible use in the food industry.
Publikováno v:
Applied Biochemistry and Microbiology. 56:893-897
The study of antimicrobial peptides (AMPs), which are considered a source of new antibiotics, have recently attracted more and more attention. AMPs combine several large groups of peptides with different spectra and different mechanisms of action on
Autor:
A. N. Fedorov, E. A. Vavilova, A. V. Lipkin, N. V. Chertova, E. G. Sadykhov, M. A. Eldarov, S. S. Zatsepin, S. Y. Filkin
Publikováno v:
Applied Biochemistry and Microbiology. 56:657-661
An effective recombinant strain of Komagataella phaffii for Bos taurus prochymosin production was obtained. A method for the isolation and purification of recombinant chymosin that includes two-stage purification via ion exchange and hydrophobic chro
Autor:
S. Y. Filkin, E. G. Sadykhov, A. A. Zenin, A N Fedorov, Vladimir Popov, A. V. Lipkin, A A Sichev, D S Bityak, N. V. Chertova
Publikováno v:
Preparative Biochemistry & Biotechnology. 50:549-555
Aim: The main purpose of this work was to develop new protocols for high yield purification of secretory phospholipase A2 (PLA2) and to investigate its biophysical properties.Materials and methods:...
Publikováno v:
Applied Biochemistry and Microbiology. 55:670-676
An efficient recombinant strain of the methylotrophic yeast Pichia pastoris was obtained; it produces active phospholipase A2 (PLA2) in a high yield. As a result of three stages of purification, a preparation of ~90% purity was obtained. The results
Autor:
D. V. Fomina, Nikolay V. Anisimov, A.A. Tarasova, Yu. A. Pirogov, E. G. Sadykhov, Olga S. Pavlova
Publikováno v:
Applied Magnetic Resonance. 50:1149-1161
Experiments on sodium (23Na) magnetic resonance imaging (MRI) of human organs, including the construction of whole body (WB) MRI, on a clinical 0.5-T scanner are described. The specificity of the research is their conduct in the presence of radio fre
Publikováno v:
Scientific Reports
The system is developed for efficient biosynthetic production of difficult-to-express polypeptides. A target polypeptide is produced fused into T. thermophilus GroEL chaperonin polypeptide chain in such a way that it is presented inside the GroEL cav
Autor:
S Y, Filkin, N V, Chertova, A A, Zenin, A V, Lipkin, A A, Sichev, D S, Bityak, E G, Sadykhov, V O, Popov, A N, Fedorov
Publikováno v:
Preparative biochemistrybiotechnology. 50(6)
Autor:
I. S. Kargov, P. D. Parshin, A. A. Pometun, Vladimir I. Tishkov, E. G. Sadykhov, S. A. Zarubina, S. Yu. Kleymenov, S. S. Savin
Publikováno v:
Moscow University Chemistry Bulletin. 73:80-84
Formate dehydrogenases (FDHs) from different sources are systematically studied in our laboratory. Over the past few years, new genes of four FDHs from pathogenic bacterium Staphylococcus aureus (SauFDH), methylotrophic thermotolerant yeast Ogataea p
Autor:
Alexandr E. Urusov, E. G. Sadykhov, Boris B. Dzantiev, Nadezhda A. Taranova, Anatoly V. Zherdev
Publikováno v:
Microchimica Acta. 184:4189-4195
This article proposes a new scheme for increasing the sensitivity of immunochromatographic assays. The scheme based on (a) recognition of dual-component gold nanoparticles (GNPs) conjugated to specific immunoglobulin G (IgG) and biotin, and (b) two a