Zobrazeno 1 - 10
of 32
pro vyhledávání: '"E F, Boldyreva"'
Autor:
Lada E. Petrovskaya, K R Birikh, E. A. Kryukova, Dmitry A. Dolgikh, S.A. Yakimov, A. V. Zlobinov, Mikhail P. Kirpichnikov, Shingarova Ln, E. F. Boldyreva, S. Sh. Gapizov
Publikováno v:
Biochemistry. Biokhimiia. 83(6)
Construction of antibody mimetics on the base of alternative scaffold proteins is a promising strategy for obtaining new products for medicine and biotechnology. The aim of our work was to optimize the cell display system for the 10th human fibronect
Autor:
S. A. Yakimov, S. Sh. Gapizov, D. A. Dolgikh, E. A. Kryukova, Lada E. Petrovskaya, Mikhail P. Kirpichnikov, E. V. Svirshchevskaya, E. P. Lukashev, E. F. Boldyreva, L. N. Shingarova
Publikováno v:
Biotechnology and applied biochemistryReferences. 66(4)
Fusion with an albumin-binding domain (ABD) of streptococcal protein G represents a popular approach for half-life extension of small protein therapeutics in the organism. To increase the circulation time of engineered αvβ3-integrin-binding protein
Autor:
S. Sh. Gapizov, K. A. Novototskaya-Vlasova, Elizaveta Rivkina, A. V. Zlobinov, Dmitry A. Dolgikh, Mikhail P. Kirpichnikov, Shingarova Ln, E. F. Boldyreva, Lada E. Petrovskaya
Publikováno v:
Extremophiles : life under extreme conditions. 22(1)
Cell surface display is a popular approach for the construction of whole-cell biocatalysts, live vaccines, and screening of combinatorial libraries. To develop a novel surface display system for the popular scaffold protein 10th human fibronectin typ
Autor:
Evgeny P. Lukashev, Mikhail P. Kirpichnikov, E. A. Kryukova, S. Sh. Gapizov, Dmitry A. Dolgikh, S.A. Yakimov, Shingarova Ln, E. F. Boldyreva, E. V. Svirschevskaya, Lada E. Petrovskaya
Publikováno v:
Russian Journal of Bioorganic Chemistry. 40:375-382
Hybrid molecules of a new type bearing a red fluorescent protein mCherry and one of the alternative scaffold proteins, the 10th human fibronectin type III domain (10Fn3), which can be used for the construction of antibody mimics with various binding
Autor:
L. N. Shingarova, S. V. Guryanova, S. A. Yakimov, E. F. Boldyreva, Alexei N. Nekrasov, E. A. Kryukova, Mikhail P. Kirpichnikov, D. A. Dolgih, Lada E. Petrovskaya
Publikováno v:
Russian Journal of Bioorganic Chemistry. 36:301-309
The tumor necrosis factor (TNF) is a proinflammatory cytokine that plays a pivotal role in the regulation of the human immune system. Studies of the TNF functional topography are a challenging task in bioengineering. We have produced genes encoding t
Autor:
Anna I. Tagvei, E. F. Boldyreva, Oksana V. Nekrasova, Anna Nazarova, Alexey V. Feofanov, Mikhail P. Kirpichnikov, Yuliya V. Korolkova, Eugene V. Grishin, Alexander S. Arseniev, Anastasia A. Ignatova
Publikováno v:
Journal of Neuroimmune Pharmacology. 4:83-91
Potassium voltage-gated channels (Kv) are considered as molecular targets in a number of serious neuronal, immune, and cardiac disorders. Search for efficient low-molecular weight modulators of Kv channel function provides a basis for the development
Autor:
Lev S. Sandakhchiev, Oksana V. Nekrasova, T. K. Aliev, Nina V. Tikunova, T. A. Batanova, A. A. Panina, L. N. Shingarova, E. F. Boldyreva, A. A. Chepurnov, V. A. Toporova, T. E. Yun, Kirpichnikov Mp
Publikováno v:
Scopus-Elsevier
A full-size human antibody to Ebola virus was constructed by joining genes encoding the constant domains of the heavy and light chains of human immunoglobulin with the corresponding DNA fragments encoding variable domains of the single-chain antibody
Autor:
L E, Petrovskaia, S Sh, Gapizov, L N, Shingarova, E A, Kriukova, E F, Boldyreva, S A, Iakimov, E V, Svirshchevskaia, E P, Lukashev, D A, Dolgikh, M P, Kirpichnikov
Publikováno v:
Bioorganicheskaia khimiia. 40(4)
In the current paper we describe a new type of hybrid molecules including red fluorescent protein mCherry and 10th type III human fibronectin domain (10Fn3) - one of the alternative scaffold proteins which can be used for the construction of antibody
Autor:
L. G. Zaitseva, O. V. Nekrasova, L. N. Shingarova, T. M. Andronova, Kobets Nv, E. F. Boldyreva, S. V. Guryanova, A. Yu. Kozlov, I. V. Kireeva, V. A. Bekhalo, E. V. Nagurskaya, Klimova Rr
Publikováno v:
Bulletin of Experimental Biology and Medicine. 140:716-719
Functional activity of macrophages and intensity of T cell immune response in mice were studied after intravaginal and intraperitoneal infection with herpes simplex virus type 1 and DNA vaccination in combination with adjuvant treatment (recombinant
Autor:
O. V. Nekrasova, Alla A. Kushch, T. M. Andronova, L. N. Shingarova, A. Yu. Kozlov, Novikov Vv, Klimova Rr, S. V. Guryanova, E. F. Boldyreva
Publikováno v:
Molecular Biology. 39:445-451
The adjuvant activity in DNA immunization against herpes simplex virus type 1 (HSV1) was studied for the granulocyte-macrophage colony-stimulating factor (GM-CSF) synthesized from an eukaryotic expression plasmid (pDNAGM-CSF) and for the synthetic gl