Výsledky vyhledávání - "E A, Benzinger"

Autor: K L, Richie, M D, Goldsborough, M M, Darfler, E A, Benzinger, M L, Lovekamp, D J, Reeder, C D, O'Connell

Publikováno v: Journal of forensic sciences. 44(6)

The Polymerase Chain Reaction (PCR) has revolutionized the analysis of DNA from a variety of sources. With its sensitivity and ability to amplify degraded DNAs and small quantities of samples, coupled with fast turn-around-time, PCR is often the anal

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::f740fd15a51d7aef4ec23383b183fb15
https://pubmed.ncbi.nlm.nih.gov/10582357

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An illustrated guide to RFLP troubleshooting

Autor: E A, Benzinger, E, Emerick, N, Grigsby, M L, Lovekamp, R, Logeman, P J, Sallee, K L, Boster, A, Rehnstrom, A K, Riech, A T, Small, J A, Glasner, T M, Coons, C, von Beroldingen

Publikováno v: Journal of forensic sciences. 43(3)

A large number of reagents and steps are required for restriction fragment length polymorphism (RFLP) analysis, which at times make determining the cause of any observed anomaly difficult. Troubleshooting problems in RFLP analysis is difficult and of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::16dc93972d9f895e05bf048c766e7cb2
https://pubmed.ncbi.nlm.nih.gov/9608706

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Products of partial digestion with Hae III. Part 1. Characterization, casework experience and confirmation of the theory of three-, four- and five-banded RFLP pattern origins using partial digestion

Autor: E A, Benzinger, E A, Emerek, N L, Grigsby, D L, Duewer, M L, Lovekamp, H, Deadman, J L, Thompson, P J, Sallee, A K, Riech

Publikováno v: Journal of forensic sciences. 42(5)

The sizes of Hae III partial digestion products at D1S7, D2S44, D4S139, D5S110, D10S28, and D17S26 were evaluated in experimentally generated partial digestions of liquid blood DNA. The partial digestion products were highly predictable, suggesting a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::de231798f48c1e696a49b16d9e0ed40b
https://pubmed.ncbi.nlm.nih.gov/9304832

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Products of partial digestion with Hae III. Part 2. Quantification

Autor: D L, Duewer, E A, Benzinger

Publikováno v: Journal of forensic sciences. 42(5)

The base pair size of the excess DNA in the smallest three partial digestion bands for the variable number of tandem repeat loci D1S7, D2S44, D4S139, D5S110, D10S28, and D17S26 has been quantitatively evaluated using data obtained from intentional pa

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::75701c41f8022254c4a6087734cc82aa
https://pubmed.ncbi.nlm.nih.gov/9304833

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Validation of probe EFD52 (D17S26) for forensic DNA analysis

Autor: M S, Nelson, E A, Benzinger, M J, Budzynski, M T, Boodee, A, Matthews, E, Buel, M B, Schwartz, C, von Beroldingen, R L, Wampler, T M, Coons

Publikováno v: Journal of forensic sciences. 41(4)

Validation studies that meet TWGDAM (The Working Group on DNA Analysis Methods) and CAC (California Association of Criminalists) guidelines for RFLP (restriction fragment length polymorphism) analysis were performed with the DNA probe EFD52 (D17S26).

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::caf27d77ccd8d963fbbe296c15bc54c4
https://pubmed.ncbi.nlm.nih.gov/8754565

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Evaluation of the specificity of alkaline phosphatase-conjugated oligonucleotide probes for forensic DNA analysis

Autor: E A, Benzinger, A K, Riech, R E, Shirley, K R, Kucharik

Publikováno v: Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society. 4(4)

Current methods of forensic DNA profiling by restriction fragment length polymorphism analysis rely on radioactive detection of DNA. The use of radioactive isotopes is complicated, expensive and requires elaborate safety precautions. Recently, non-ra

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::98fab0abedaa89c131b8b39cbefb64ca
https://pubmed.ncbi.nlm.nih.gov/7599252

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Time course and inhibitors of Hae III digestion in the forensic laboratory

Autor: E A, Benzinger, R E, Shirley, A K, Riech, P J, Sallee, K L, Boster, K C, Lumney

Publikováno v: Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society. 4(4)

Forensic restriction fragment length polymorphism (RFLP) profiling has become standardized in the United States and Canada to the extent that all federal, state and local forensic laboratories employ the restriction enzyme Hae III. Forensic DNA sampl

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::5c2099f7baba4d0ae42e7e6d352b1480
https://pubmed.ncbi.nlm.nih.gov/7599254

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