Zobrazeno 1 - 10
of 52
pro vyhledávání: '"E, Laurensse"'
Autor:
G J, Peters, E, Laurensse, H W, Steinbusch, J, De Vente, K, Smid, C L, Van der Wilt, H M, Pinedo
Publikováno v:
Anticancer research. 13(4)
5-Fluoro-2'-deoxyuridine-5'-monophosphate (FdUMP) is the active metabolite of the anticancer agent 5-fluorouracil (5FU). Antibodies against a conjugate of thyroglobulin with acetylated FdUMP were raised in 4 rabbits. The maximal titer was reached aft
Publikováno v:
Analytical Biochemistry. 127:340-345
A flow-through system in which monolayer cells, growing on a 50-cm 2 glass surface, are in contact with a film of medium with a thickness of 0.14 mm, is described. For murine B16 melanoma cells, the loading capacity is 8.10 6 cells. The flow-through
Publikováno v:
Investigational new drugs. 5(3)
The mechanism of action of NSC 368390 (DUP-785, 6-fluoro-2-(2'-fluoro-1, 1'-biphenyl-4-yl)-3-methyl-4-quinoline carboxylic acid sodium salt) was studied using three different approaches. First, we studied growth inhibition by DUP-785 in L1210 leukemi
Publikováno v:
Cancer research. 46(1)
Six cell lines differing in histological origin were studied regarding the growth inhibitory effect of fluoropyrimidines in relation to their metabolism. The human colon carcinoma cell line WiDr was most sensitive to 5-fluorouracil (FUra) (50% growth
Publikováno v:
Advances in experimental medicine and biology.
Autor:
G.J. Peters, H.M. Pinedo, A.H. Loonen, E. Laurensse, Albert Leyva, F.C. de Waal, Gilberto Schwartsmann
Publikováno v:
Biochemical pharmacology. 37(17)
DUP 785 (NSC 368390; Brequinar sodium) is a new inhibitor of pyrimidine de novo biosynthesis with antitumor activity against several experimental tumors. DUP 785 inhibits the mitochondrial enzyme dihydroorotate dehydrogenase, blocking the conversion
Publikováno v:
Analytical biochemistry. 161(1)
A new method to assay the mitochondrial pyrimidine de novo enzyme, dihydroorotate (DHO) dehydrogenase, which catalyzes the dehydrogenation of DHO, with orotic acid as the product was developed. The assay was optimized using a rat liver mitochondrial
Publikováno v:
Pharmaceutical research. 4(2)
The effect of high-dose uridine on body temperatures of rabbits and man has been studied in relation to plasma concentrations of uridine and its catabolite uracil. Uridine induced fever in both rabbits and man. High-dose cytidine had no influence on
Publikováno v:
European journal of cancerclinical oncology. 20(11)
5-Fluorouracil (5FU) metabolism was studied in intact cancer cells kept in suspension by incubation with tritiated 5FU. Metabolites were analyzed using various chromatographic procedures, including a one-directional separation on PEI-cellulose sheets
Publikováno v:
Advances in experimental medicine and biology.