Rapid Organoid Reconstitution by Chemical Micromolding

Autor: James C. Garbe, Mark A. LaBarge, Robert J. Weber, Alec E. Cerchiari, Lucas S. Delannoy, Tejal A. Desai, Zev J. Gartner

Publikováno v: ACS biomaterials scienceengineering. 2(11)

Purified populations of cells can be reconstituted into organoids that recapitulate aspects of their in vivo structure and function. These organoids are useful as models of healthy and diseased tissue in the basic sciences, in vitro screens, and rege

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1d8dcf11e550d5896388dd4fcb38daca
https://pubmed.ncbi.nlm.nih.gov/33440521

Fabrication of 3‐D Reconstituted Organoid Arrays by DNA‐Programmed Assembly of Cells (DPAC)

Autor: Zev J. Gartner, Noel Y. Jee, Justin Farlow, Michael E. Todhunter, Alec E. Cerchiari, Robert J. Weber

Publikováno v: Current Protocols in Chemical Biology

Tissues are the organizational units of function in metazoan organisms. Tissues comprise an assortment of cellular building blocks, soluble factors, and extracellular matrix (ECM) composed into specific three-dimensional (3-D) structures. The capacit

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e4220dc037b1b1810e61032e76c06758
https://doi.org/10.1002/cpch.8

Programmed synthesis of three-dimensional tissues

Autor: Maxwell C. Coyle, Alex J. Hughes, Noel Y. Jee, James C. Garbe, Alec E. Cerchiari, Zev J. Gartner, Tejal A. Desai, Mark A. LaBarge, Justin Farlow, Michael E. Todhunter

Publikováno v: Nature Methods. 12:975-981

Reconstituting tissues from their cellular building blocks facilitates the modeling of morphogenesis, homeostasis and disease in vitro. Here we describe DNA-programmed assembly of cells (DPAC), a method to reconstitute the multicellular organization

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::fe1273b57408f527b878c53df2ff6e3e
https://doi.org/10.1038/nmeth.3553

A strategy for tissue self-organization that is robust to cellular heterogeneity and plasticity

Autor: Alec E. Cerchiari, Tejal A. Desai, Kyle E. Broaders, James C. Garbe, Michael E. Todhunter, Matt Thomson, Zev J. Gartner, Mark A. LaBarge, Noel Y. Jee, Donna M. Peehl

Publikováno v: Proceedings of the National Academy of Sciences. 112:2287-2292

Developing tissues contain motile populations of cells that can self-organize into spatially ordered tissues based on differences in their interfacial surface energies. However, it is unclear how self-organization by this mechanism remains robust whe

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::785fe993bb5bccfa93efa977f1b9def4
https://doi.org/10.1073/pnas.1410776112

Novel Functionalization of Discrete Polymeric Biomaterial Microstructures for Applications in Imaging and Three-Dimensional Manipulation

Autor: James Hawkins, Gerd Melkus, Alec E. Cerchiari, Tejal A. Desai, James R. Pinney

Publikováno v: ACS Applied Materials & Interfaces
ACS applied materials & interfaces, vol 6, iss 16
Pinney, JR; Melkus, G; Cerchiari, A; Hawkins, J; & Desai, TA. (2014). Novel functionalization of discrete polymeric biomaterial microstructures for applications in imaging and three-dimensional manipulation. ACS Applied Materials and Interfaces, 6(16), 14477-14485. doi: 10.1021/am503778t. UCSF: Retrieved from: http://www.escholarship.org/uc/item/88q9j2hh

Adapting ways to functionalize polymer materials is becoming increasingly important to their implementation in translational biomedical sciences. By tuning the mechanical, chemical, and biological qualities of these materials, their applications can

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::21692905d9fbde46b3c85881356b553c
http://europepmc.org/articles/PMC4149329