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Inter-laboratory quality control for hormone-dependent gene expression in human breast tumors using real-time reverse transcription-polymerase chain reaction

Autor: Bernard Asselain, Carine Tran-Perennou, Rosette Lidereau, Ivan Bièche, S. Vignaud, Véronique Becette, Henri Magdelenat, E Boudou, P. de Cremoux, F. Spyratos, Brigitte Sigal-Zafrani

Publikováno v: Endocrine-Related Cancer. 11:489-495

Quantitative reverse transcription-polymerase chain reaction (RT-PCR) used to detect minor changes in specific mRNA concentrations may be associated with poor reproducibility. Stringent quality control is therefore essential at each step of the proto

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bc54840e526d1334cf5bdb13b3bfb8b2
https://doi.org/10.1677/erc.1.00808

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Validation of real-time RT-PCR for analysis of human breast cancer cell lines resistant or sensitive to treatment with antiestrogens

Autor: E Boudou, B L Brockdorff, Nils Brünner, Henri Magdelenat, P. de Cremoux, Carine Tran-Perennou, Anne E. Lykkesfeldt

Publikováno v: Endocrine-related cancer. :409-418

Using a quantitative real-time RT-PCR technique we have compared the expression of a number of genes in two different human breast cancer model systems for development of acquired resistance to antiestrogens. The model system developed at the Danish

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5ce90d1ffe6f23a7a519b31d99125d24
https://doi.org/10.1677/erc.0.0100409

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High sensitivity and specificity of immunohistochemistry for the detection of hormone receptors in breast carcinoma: comparison with biochemical determination in a prospective study of 793 cases

Autor: Xavier Sastre-Garau, M H Aubriot, André Nicolas, Anne Vincent-Salomon, P. de Cremoux, Brigitte Zafrani, E Mouret, Henri Magdelenat, E Boudou, Y. De Rycke

Publikováno v: Histopathology. 37:536-545

Aims The hormone receptor (HR) status of breast cancer is an important prognostic factor and predictive parameter of the response to hormone therapy. Enzyme immunoassay (EIA) is currently the standard for determination of HR, but immunohistochemistry

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::f46e66cda2d6d0b56050545ed9fa5812
https://doi.org/10.1046/j.1365-2559.2000.01006.x

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Homogeneously staining regions in 223 breast carcinomas: cytogenetic and clinicopathological correlations

Autor: E Boudou, Y Dericke, M. Gerbault-Seureau, B Zafrani, J. Bernardino, Bernard Dutrillaux, Henri Magdelenat

Publikováno v: British Journal of Cancer

A correlation analysis was performed on 223 breast carcinomas to assess the relationships between gene amplification, karyotypic and clinicopathological features. Homogeneously staining region (HSR) is the most frequent form of amplification found in

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e1b5d142c8180fb3b4a19eedbb3df25e
https://doi.org/10.1038/bjc.1998.657

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High sensitivity and specificity of immunohistochemistry for the detection of hormone receptors in breast carcinoma: comparison with biochemical determination in a prospective study of 793 cases

Autor: B, Zafrani, M H, Aubriot, E, Mouret, P, De Crémoux, Y, De Rycke, A, Nicolas, E, Boudou, A, Vincent-Salomon, H, Magdelénat, X, Sastre-Garau

Publikováno v: Histopathology. 37(6)

The hormone receptor (HR) status of breast cancer is an important prognostic factor and predictive parameter of the response to hormone therapy. Enzyme immunoassay (EIA) is currently the standard for determination of HR, but immunohistochemistry (IHC

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::838672aff4c62b78d14a3d4937e0ce85
https://pubmed.ncbi.nlm.nih.gov/11260308

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Detection of MUC1-expressing mammary carcinoma cells in the peripheral blood of breast cancer patients by real-time polymerase chain reaction

Autor: P, de Cremoux, J M, Extra, M G, Denis, J Y, Pierga, E, Bourstyn, C, Nos, K B, Clough, E, Boudou, E C, Martin, A, Müller, P, Pouillart, H, Magdelénat

Publikováno v: Clinical cancer research : an official journal of the American Association for Cancer Research. 6(8)

We have prospectively analyzed blood samples of 122 patients with breast disease for the presence of circulating expressing MUC1 cells before and after treatment. Among them, 28 patients had histologically confirmed benign breast disease (group 1), 3

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::f75db1a439ce81a0414280968d69ca17
https://pubmed.ncbi.nlm.nih.gov/10955792

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