Zobrazeno 1 - 10
of 20
pro vyhledávání: '"Dwight Winters"'
Autor:
Emily K. Makowski, Hsin-Ting Chen, Tiexin Wang, Lina Wu, Jie Huang, Marissa Mock, Patrick Underhill, Emma Pelegri-O’Day, Erick Maglalang, Dwight Winters, Peter M. Tessier
Publikováno v:
mAbs, Vol 16, Iss 1 (2024)
ABSTRACTEarly identification of antibody candidates with drug-like properties is essential for simplifying the development of safe and effective antibody therapeutics. For subcutaneous administration, it is important to identify candidates with low s
Externí odkaz:
https://doaj.org/article/36271266403a4691a2dd72e35b369f74
Autor:
Marissa Mock, Alex W. Jacobitz, Christopher James Langmead, Athena Sudom, Daniel Yoo, Sara C. Humphreys, Mai Alday, Larysa Alekseychyk, Nicolas Angell, Vivian Bi, Hannah Catterall, Chen-Chun Chen, Hui-Ting Chou, Kip P. Conner, Kevin D. Cook, Ana R. Correia, Andrew Dykstra, Sudipa Ghimire-Rijal, Kevin Graham, Peter Grandsard, Joon Huh, John O. Hui, Mani Jain, Victoria Jann, Lei Jia, Sheree Johnstone, Neelam Khanal, Carl Kolvenbach, Linda Narhi, Rupa Padaki, Emma M. Pelegri-O’Day, Wei Qi, Vladimir Razinkov, Austin J. Rice, Richard Smith, Christopher Spahr, Jennitte Stevens, Yax Sun, Veena A. Thomas, Sarah van Driesche, Robert Vernon, Victoria Wagner, Kenneth W. Walker, Yangjie Wei, Dwight Winters, Melissa Yang, Iain D. G. Campuzano
Publikováno v:
mAbs, Vol 15, Iss 1 (2023)
ABSTRACTBiologic drug discovery pipelines are designed to deliver protein therapeutics that have exquisite functional potency and selectivity while also manifesting biophysical characteristics suitable for manufacturing, storage, and convenient admin
Externí odkaz:
https://doaj.org/article/c7e86c82a6684b888888da7d561e8891
Autor:
Randy Hecht, Yue-Sheng Li, Jeonghoon Sun, Ed Belouski, Michael Hall, Todd Hager, Junming Yie, Wei Wang, Dwight Winters, Stephen Smith, Chris Spahr, Lei-Ting Tam, Zhongnan Shen, Shanaka Stanislaus, Narumol Chinookoswong, Yvonne Lau, Allen Sickmier, Mark Leo Michaels, Thomas Boone, Murielle M Véniant, Jing Xu
Publikováno v:
PLoS ONE, Vol 7, Iss 11, p e49345 (2012)
Fibroblast growth factor 21 (FGF21) is a promising drug candidate for the treatment of type 2 diabetes. However, the use of wild type native FGF21 is challenging due to several limitations. Among these are its short half-life, its susceptibility to i
Externí odkaz:
https://doaj.org/article/4b03784bd0594b29a2d0316fd49362ba
Autor:
Dwight Winters, Todd Hager, Michael Hall, Shanaka Stanislaus, Murielle M. Véniant, Randy Ira Hecht, Chris Spahr, Lei Zhou, Jing Xu, Wei Wang, Yang Li, Junming Yie, Yue-Sheng Li, Liying Deng, Jennifer Weiszmann, Stephen Smith
Publikováno v:
Endocrinology. 158:1314-1327
Fibroblast growth factor (FGF) 21 is a natural hormone that modulates glucose, lipid, and energy metabolism. Previously, we engineered an Fc fusion FGF21 variant with two mutations, Fc-FGF21(RG), to extend the half-life and reduce aggregation and in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::31e301d1ddf343756228eca215f2028d
https://doi.org/10.1210/en.2016-1917
https://doi.org/10.1210/en.2016-1917
Publikováno v:
Protein expression and purification. 165
Affinity purification, such as Protein A (ProA) followed by size exclusion chromatography (SEC) remains a popular method to obtain research scale proteins. With the need for higher throughput protein production increasing for discovery research, ther
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5057ce736847cff798bfa9bb0fa9bd82
https://pubmed.ncbi.nlm.nih.gov/31499166
https://pubmed.ncbi.nlm.nih.gov/31499166
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2025
In vitro protein folding can be employed to produce complex proteins expressed as insoluble inclusion bodies in E. coli from laboratory to commercial scale. Often the most challenging step is identification of renaturation conditions that will enable
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::96024526abd10af79caa3c909cd03af9
https://pubmed.ncbi.nlm.nih.gov/31267451
https://pubmed.ncbi.nlm.nih.gov/31267451
Publikováno v:
Methods in Molecular Biology ISBN: 9781493996230
In vitro protein folding can be employed to produce complex proteins expressed as insoluble inclusion bodies in E. coli from laboratory to commercial scale. Often the most challenging step is identification of renaturation conditions that will enable
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e451a72d46e7447e97138ad414d42f15
https://doi.org/10.1007/978-1-4939-9624-7_6
https://doi.org/10.1007/978-1-4939-9624-7_6
Publikováno v:
Protein Expression and Purification. 120:138-147
Modern molecular genetics technology has made it possible to swiftly sequence, clone and mass-produce recombinant DNA for the purpose of expressing heterologous genes of interest; however, recombinant protein production systems have struggled to keep
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7abf1b18a8f9cfe3c55f11363a2881b8
https://doi.org/10.1016/j.pep.2015.11.015
https://doi.org/10.1016/j.pep.2015.11.015
Publikováno v:
Journal of Chromatography A. 1424:51-58
There has been a great emphasis on developing higher-throughput protein purification techniques to screen potential human therapeutics faster and more efficiently. Not only is it desirable to have high-throughput purification for initial screens but
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f4b3aadccf7bd2919ef6e3e9ce887aea
https://doi.org/10.1016/j.chroma.2015.10.092
https://doi.org/10.1016/j.chroma.2015.10.092
Autor:
Dwight Winters, Brooke M. Rock, Yunan Wang, Marcus Soto, Josh T. Pearson, Mei Han, Dan A. Rock
Publikováno v:
Analytical biochemistry. 539
Protein engineering is at an all-time high in biopharmaceutics. As a result, absorption, distribution, metabolism and excretion (ADME) of proteins has become more important to understand in the context of engineering strategies to optimize therapeuti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::58f6d95c723f92ebc46f5cc1d95d8275
https://pubmed.ncbi.nlm.nih.gov/29029979
https://pubmed.ncbi.nlm.nih.gov/29029979