Experimental test of connector rotation during DNA packaging into bacteriophage phi29 capsids.

Autor: Thorsten Hugel, Jens Michaelis, Craig L Hetherington, Paul J Jardine, Shelley Grimes, Jessica M Walter, Wayne Falk, Dwight L Anderson, Carlos Bustamante

Publikováno v: PLoS Biology, Vol 5, Iss 3, p e59 (2007)

The bacteriophage phi29 generates large forces to compact its double-stranded DNA genome into a protein capsid by means of a portal motor complex. Several mechanical models for the generation of these high forces by the motor complex predict coupling

Externí odkaz: https://doaj.org/article/c07a6fcd72fd47bdaa6c3424c0845db8

Crystal and cryoEM structural studies of a cell wall degrading enzyme in the bacteriophage φ29 tail

Autor: Valorie D. Bowman, Michael G. Rossmann, Dwight L. Anderson, Marc C. Morais, Daniel N. Cohen, Ye Xiang

Publikováno v: Proceedings of the National Academy of Sciences. 105:9552-9557

The small bacteriophage φ29 must penetrate the ≈250-Å thick external peptidoglycan cell wall and cell membrane of the Gram-positive Bacillus subtilis , before ejecting its dsDNA genome through its tail into the bacterial cytoplasm. The tail of b

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::839720e678e9144d35da6d3c2be8ca07
https://doi.org/10.1073/pnas.0803787105

Three-dimensional architecture of the bacteriophage φ29 packaged genome and elucidation of its packaging process

Autor: Kenneth H. Downing, Shelley Grimes, Luis R. Comolli, Andrew J. Spakowitz, Paul J. Jardine, Carlos Bustamante, Dwight L. Anderson, Cristina Siegerist

Publikováno v: Comolli, Luis R.; Spakowitz, Andrew; Siegerist, Cristina E.; Grimes, Shelley; Jardine, Paul; Anderson, Dwight; et al.(2006). Three-Dimensional Architecture of the Bacteriophage phi29 Packaged Genome and Elucidation of its Packaging Process. Lawrence Berkeley National Laboratory: Lawrence Berkeley National Laboratory. Retrieved from: http://www.escholarship.org/uc/item/3mb1s4b2

Genetic and physiological studies of dsDNA bacteriophages have established their assembly pathways in some detail, yet the precise mechanism of DNA packaging remains obscure. We follow the packaging process step-wise, from the initial state in which

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4e82beec566ba8f833339002a08fad0c
https://doi.org/10.1016/j.virol.2007.07.035

Ionic effects on viral DNA packaging and portal motor function in bacteriophage φ29

Autor: Dwight L. Anderson, Paul J. Jardine, John Peter Rickgauer, Shelley Grimes, Douglas E. Smith, Derek N. Fuller

Publikováno v: Proceedings of the National Academy of Sciences. 104:11245-11250

In many viruses, DNA is confined at such high density that its bending rigidity and electrostatic self-repulsion present a strong energy barrier in viral assembly. Therefore, a powerful molecular motor is needed to package the DNA into the viral caps

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::f59bb162180c135501b64be7328044b1
https://doi.org/10.1073/pnas.0701323104

Structural changes of bacteriophage φ29 upon DNA packaging and release

Autor: Michael G. Rossmann, Shelley Grimes, Anthony J. Battisti, Paul J. Jardine, Dwight L. Anderson, Marc C. Morais, Ye Xiang

Publikováno v: The EMBO Journal. 25:5229-5239

Cryo-electron microscopy three-dimensional reconstructions have been made of mature and of emptied bacteriophage phi29 particles without making symmetry assumptions. Comparisons of these structures with each other and with the phi29 prohead indicate

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d640c35ec918f3b1a821c008d8e135d2
https://doi.org/10.1038/sj.emboj.7601386