Structure and mechanism of a canonical poly(ADP-ribose) glycohydrolase

Autor: Dunstan MS, Barkauskaite E, Lafite P, Knezevic CE, Brassington A, Ahel M, Hergenrother PJ, Leys D, Ahel I.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=od______2295::82fbb62547533f0caf540cfb3674215a
http://www.manchester.ac.uk/escholar/uk-ac-man-scw:162610

SpeedyGenesXL: an Automated, High-Throughput Platform for the Preparation of Bespoke Ultralarge Variant Libraries for Directed Evolution.

Autor: Sadler JC; School of Chemistry, The University of Manchester, Manchester, UK.; Institute of Quantitative Biology, Biochemistry and Biotechnology, School of Biological Sciences, University of Edinburgh, Edinburgh, UK.; The Manchester Institute of Biotechnology, The University of Manchester, Manchester, UK.; Centre for the Synthetic Biology of Fine and Specialty Chemicals (SYNBIOCHEM), The University of Manchester, Manchester, UK.; Mellizyme Biotechnology Ltd, Liverpool Science Park, Liverpool, UK., Swainston N; Manchester Institute of Biotechnology, University of Manchester, Manchester, UK., Dunstan MS; School of Chemistry, The University of Manchester, Manchester, UK.; The Manchester Institute of Biotechnology, The University of Manchester, Manchester, UK.; Centre for the Synthetic Biology of Fine and Specialty Chemicals (SYNBIOCHEM), The University of Manchester, Manchester, UK., Currin A; Manchester Institute of Biotechnology, University of Manchester, Manchester, UK., Kell DB; Department of Biochemistry and Systems Biology, Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool, UK. dbk@liv.ac.uk.; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kgs Lyngby, Denmark. dbk@liv.ac.uk.

Publikováno v: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2461, pp. 67-83.

A plasmid toolset for CRISPR-mediated genome editing and CRISPRi gene regulation in Escherichia coli.

Autor: Jervis AJ; Manchester Centre for Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, University of Manchester, Manchester, M1 7DN, UK., Hanko EKR; Manchester Centre for Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, University of Manchester, Manchester, M1 7DN, UK., Dunstan MS; Manchester Centre for Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, University of Manchester, Manchester, M1 7DN, UK., Robinson CJ; Manchester Centre for Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, University of Manchester, Manchester, M1 7DN, UK., Takano E; Manchester Centre for Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, University of Manchester, Manchester, M1 7DN, UK., Scrutton NS; Manchester Centre for Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, University of Manchester, Manchester, M1 7DN, UK.

Publikováno v: Microbial biotechnology [Microb Biotechnol] 2021 May; Vol. 14 (3), pp. 1120-1129. Date of Electronic Publication: 2021 Mar 12.

Rapid prototyping of microbial production strains for the biomanufacture of potential materials monomers.

Autor: Robinson CJ; Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: Christopher.Robinson@manchester.ac.uk., Carbonell P; Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: pablo.carbonell@manchester.ac.uk., Jervis AJ; Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: Adrian.Jervis@manchester.ac.uk., Yan C; Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: cunyu.yan@manchester.ac.uk., Hollywood KA; Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: katherine.hollywood@manchester.ac.uk., Dunstan MS; Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: Mark.Dunstan@manchester.ac.uk., Currin A; Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: andrew.currin@manchester.ac.uk., Swainston N; Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: Neil.Swainston@manchester.ac.uk., Spiess R; Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: Reynard.Spiess@manchester.ac.uk., Taylor S; Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: Sandra.Taylor@manchester.ac.uk., Mulherin P; Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: paul.mulherin@manchester.ac.uk., Parker S; Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: steven.parker@manchester.ac.uk., Rowe W; Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: William.rowe@manchester.ac.uk., Matthews NE; Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK; Manchester Institute of Innovation Research, Alliance Manchester Business School, The University of Manchester, Manchester, M15 6PB, UK. Electronic address: n.matthews@manchester.ac.uk., Malone KJ; Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: Kirk.Malone@manchester.ac.uk., Le Feuvre R; Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK. Electronic address: r.a.le-feuvre@manchester.ac.uk., Shapira P; Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK; Manchester Institute of Innovation Research, Alliance Manchester Business School, The University of Manchester, Manchester, M15 6PB, UK. Electronic address: PShapira@manchester.ac.uk., Barran P; Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK; Department of Chemistry, The University of Manchester, Manchester, M13 9PL, UK. Electronic address: perdita.barran@manchester.ac.uk., Turner NJ; Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK; Department of Chemistry, The University of Manchester, Manchester, M13 9PL, UK. Electronic address: Nicholas.Turner@manchester.ac.uk., Micklefield J; Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK; Department of Chemistry, The University of Manchester, Manchester, M13 9PL, UK. Electronic address: jason.micklefield@manchester.ac.uk., Breitling R; Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK; Department of Chemistry, The University of Manchester, Manchester, M13 9PL, UK. Electronic address: Rainer.Breitling@manchester.ac.uk., Takano E; Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK; Department of Chemistry, The University of Manchester, Manchester, M13 9PL, UK. Electronic address: Eriko.Takano@manchester.ac.uk., Scrutton NS; Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK; Department of Chemistry, The University of Manchester, Manchester, M13 9PL, UK. Electronic address: Nigel.Scrutton@manchester.ac.uk.

Publikováno v: Metabolic engineering [Metab Eng] 2020 Jul; Vol. 60, pp. 168-182. Date of Electronic Publication: 2020 Apr 23.

Highly multiplexed, fast and accurate nanopore sequencing for verification of synthetic DNA constructs and sequence libraries.

Autor: Currin A; Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester M1 7DN, UK.; School of Natural Sciences, Department of Chemistry, Faculty of Science and Engineering, The University of Manchester, Manchester M13 9PL, UK., Swainston N; Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester M1 7DN, UK.; School of Natural Sciences, Department of Chemistry, Faculty of Science and Engineering, The University of Manchester, Manchester M13 9PL, UK.; Institute of Integrative Biology, University of Liverpool, Liverpool L69 7ZB, UK., Dunstan MS; Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester M1 7DN, UK.; School of Natural Sciences, Department of Chemistry, Faculty of Science and Engineering, The University of Manchester, Manchester M13 9PL, UK., Jervis AJ; Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester M1 7DN, UK.; School of Natural Sciences, Department of Chemistry, Faculty of Science and Engineering, The University of Manchester, Manchester M13 9PL, UK., Mulherin P; Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester M1 7DN, UK.; School of Natural Sciences, Department of Chemistry, Faculty of Science and Engineering, The University of Manchester, Manchester M13 9PL, UK., Robinson CJ; Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester M1 7DN, UK.; School of Natural Sciences, Department of Chemistry, Faculty of Science and Engineering, The University of Manchester, Manchester M13 9PL, UK., Taylor S; Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester M1 7DN, UK.; School of Natural Sciences, Department of Chemistry, Faculty of Science and Engineering, The University of Manchester, Manchester M13 9PL, UK., Carbonell P; Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester M1 7DN, UK.; School of Natural Sciences, Department of Chemistry, Faculty of Science and Engineering, The University of Manchester, Manchester M13 9PL, UK., Hollywood KA; Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester M1 7DN, UK.; School of Natural Sciences, Department of Chemistry, Faculty of Science and Engineering, The University of Manchester, Manchester M13 9PL, UK., Yan C; Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester M1 7DN, UK.; School of Natural Sciences, Department of Chemistry, Faculty of Science and Engineering, The University of Manchester, Manchester M13 9PL, UK., Takano E; Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester M1 7DN, UK.; School of Natural Sciences, Department of Chemistry, Faculty of Science and Engineering, The University of Manchester, Manchester M13 9PL, UK., Scrutton NS; Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester M1 7DN, UK.; School of Natural Sciences, Department of Chemistry, Faculty of Science and Engineering, The University of Manchester, Manchester M13 9PL, UK., Breitling R; Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM), Manchester Institute of Biotechnology, The University of Manchester, Manchester M1 7DN, UK.; School of Natural Sciences, Department of Chemistry, Faculty of Science and Engineering, The University of Manchester, Manchester M13 9PL, UK.

Publikováno v: Synthetic biology (Oxford, England) [Synth Biol (Oxf)] 2019 Oct 29; Vol. 4 (1), pp. ysz025. Date of Electronic Publication: 2019 Oct 29 (Print Publication: 2019).