Zobrazeno 1 - 10
of 50
pro vyhledávání: '"Douglas S Kim"'
Autor:
Hod Dana, Ondrej Novak, Michael Guardado-Montesino, James W Fransen, Amy Hu, Bart G Borghuis, Caiying Guo, Douglas S Kim, Karel Svoboda
Publikováno v:
PLoS ONE, Vol 13, Iss 10, p e0205444 (2018)
Calcium imaging is commonly used to measure the neural activity of large groups of neurons in mice. Genetically encoded calcium indicators (GECIs) can be delivered for this purpose using non-invasive genetic methods. Compared to viral gene transfer,
Externí odkaz:
https://doaj.org/article/8b88862f4edf4231bf55e26405b11718
Autor:
Hod Dana, Boaz Mohar, Yi Sun, Sujatha Narayan, Andrew Gordus, Jeremy P Hasseman, Getahun Tsegaye, Graham T Holt, Amy Hu, Deepika Walpita, Ronak Patel, John J Macklin, Cornelia I Bargmann, Misha B Ahrens, Eric R Schreiter, Vivek Jayaraman, Loren L Looger, Karel Svoboda, Douglas S Kim
Publikováno v:
eLife, Vol 5 (2016)
Genetically encoded calcium indicators (GECIs) allow measurement of activity in large populations of neurons and in small neuronal compartments, over times of milliseconds to months. Although GFP-based GECIs are widely used for in vivo neurophysiolog
Externí odkaz:
https://doaj.org/article/ab34ae2c5e2b47b2a8b288f3d69d627e
Autor:
Mark J Henderson, Heather A Baldwin, Christopher A Werley, Stefano Boccardo, Leslie R Whitaker, Xiaokang Yan, Graham T Holt, Eric R Schreiter, Loren L Looger, Adam E Cohen, Douglas S Kim, Brandon K Harvey
Publikováno v:
PLoS ONE, Vol 10, Iss 10, p e0139273 (2015)
Endoplasmic reticulum calcium homeostasis is critical for cellular functions and is disrupted in diverse pathologies including neurodegeneration and cardiovascular disease. Owing to the high concentration of calcium within the ER, studying this subce
Externí odkaz:
https://doaj.org/article/161ea35e86594287bc3d469f5096851a
Autor:
Hod Dana, Tsai-Wen Chen, Amy Hu, Brenda C Shields, Caiying Guo, Loren L Looger, Douglas S Kim, Karel Svoboda
Publikováno v:
PLoS ONE, Vol 9, Iss 9, p e108697 (2014)
Genetically-encoded calcium indicators (GECIs) facilitate imaging activity of genetically defined neuronal populations in vivo. The high intracellular GECI concentrations required for in vivo imaging are usually achieved by viral gene transfer using
Externí odkaz:
https://doaj.org/article/84ee2ea372c043e3875da1b639043a5b
Autor:
Jasper eAkerboom, Nicole eCarreras Calderón, Lin eTian, Sebastian eWabnig, Matthias ePrigge, Johan eTolö, Andrew eGordus, Michael B Orger, Kristen E Severi, John J Macklin, Ronak ePatel, Stefan R Pulver, Trevor J Wardill, Elisabeth eFischer, Christina eSchüler, Tsai-Wen eChen, Karen S Sarkisyan, Jonathan S Marvin, Cornelia I Bargmann, Douglas S Kim, Sebastian eKügler, Leon eLagnado, Peter eHegemann, Alexander eGottschalk, Eric R Schreiter, Loren L Looger
Publikováno v:
Frontiers in Molecular Neuroscience, Vol 6 (2013)
Genetically encoded calcium indicators (GECIs) are powerful tools for systems neuroscience. Here we describe red, single-wavelength GECIs, RCaMPs, engineered from circular permutation of the thermostable red fluorescent protein mRuby. High-resolution
Externí odkaz:
https://doaj.org/article/46e541bc41ce4dd2bdcb29ec64a7b4d7
Autor:
Trevor J Wardill, Tsai-Wen Chen, Eric R Schreiter, Jeremy P Hasseman, Getahun Tsegaye, Benjamin F Fosque, Reza Behnam, Brenda C Shields, Melissa Ramirez, Bruce E Kimmel, Rex A Kerr, Vivek Jayaraman, Loren L Looger, Karel Svoboda, Douglas S Kim
Publikováno v:
PLoS ONE, Vol 8, Iss 10, p e77728 (2013)
Fluorescent protein-based sensors for detecting neuronal activity have been developed largely based on non-neuronal screening systems. However, the dynamics of neuronal state variables (e.g., voltage, calcium, etc.) are typically very rapid compared
Externí odkaz:
https://doaj.org/article/260c05b0e92b4cd0a03c4417df7ccb10
Autor:
Hong Hao, Douglas S Kim, Bernward Klocke, Kory R Johnson, Kairong Cui, Norimoto Gotoh, Chongzhi Zang, Janina Gregorski, Linn Gieser, Weiqun Peng, Yang Fann, Martin Seifert, Keji Zhao, Anand Swaroop
Publikováno v:
PLoS Genetics, Vol 8, Iss 4, p e1002649 (2012)
A stringent control of homeostasis is critical for functional maintenance and survival of neurons. In the mammalian retina, the basic motif leucine zipper transcription factor NRL determines rod versus cone photoreceptor cell fate and activates the e
Externí odkaz:
https://doaj.org/article/00af419cad1b4925b1045418b2ca1f58
Autor:
Loren L. Looger, Douglas S. Kim, Eric R. Schreiter, Allan M. Wong, Chi-Yu Lee, Abhi Aggarwal, Jeong Jun Kim, Emiliano Jimenez Marquez, Allison M. Ahrens, Jonathan S. Marvin, Kaspar Podgorski, Yajie Liang, Daniel Bushey, Manuel A. Mohr, John J. Macklin, Getahun Tsegaye, Jerry L. Chen, Ronak Patel, Arthur Tsang
Publikováno v:
Nature methods
Femtosecond lasers at fixed wavelengths above 1,000 nm are powerful, stable and inexpensive, making them promising sources for two-photon microscopy. Biosensors optimized for these wavelengths are needed for both next-generation microscopes and affor
Autor:
Ahmed S. Abdelfattah, Rosario Valenti, Jihong Zheng, Allan Wong, GENIE Project Team, Kaspar Podgorski, Minoru Koyama, Douglas S. Kim, Eric R. Schreiter
Publikováno v:
Nature Communications
Nature Communications, Vol 11, Iss 1, Pp 1-8 (2020)
Nature Communications, Vol 11, Iss 1, Pp 1-8 (2020)
Imaging membrane voltage from genetically defined cells offers the unique ability to report spatial and temporal dynamics of electrical signaling at cellular and circuit levels. Here, we present a general approach to engineer electrochromic fluoresce
Autor:
Yi, Shen, Hod, Dana, Ahmed S, Abdelfattah, Ronak, Patel, Jamien, Shea, Rosana S, Molina, Bijal, Rawal, Vladimir, Rancic, Yu-Fen, Chang, Lanshi, Wu, Yingche, Chen, Yong, Qian, Matthew D, Wiens, Nathan, Hambleton, Klaus, Ballanyi, Thomas E, Hughes, Mikhail, Drobizhev, Douglas S, Kim, Minoru, Koyama, Eric R, Schreiter, Robert E, Campbell
Publikováno v:
BMC Biology
Background Genetically encoded calcium ion (Ca2+) indicators (GECIs) are indispensable tools for measuring Ca2+ dynamics and neuronal activities in vitro and in vivo. Red fluorescent protein (RFP)-based GECIs have inherent advantages relative to gree