Zobrazeno 1 - 10
of 20
pro vyhledávání: '"Douglas J. H. Olson"'
Publikováno v:
Industrial Crops and Products. 37:186-194
Triacylglycerol-estolides are components of the storage oil of certain plant and fungal species and are generally associated with the presence of fatty acids containing hydroxyl groups. These unusual acyl-glycerols can easily go undetected when oils
Publikováno v:
Plant Science. 176:99-104
Different protein extraction methods have been developed for plant proteome analysis but their compatibility with mass spectrometry has rarely been tested. We evaluated four protein extraction methods, i.e., trichloroacetic acid (TCA)–acetone, phen
Publikováno v:
Journal of Experimental Botany. 58:3525-3535
In flowering plants, pollen grains are produced in the anther and released to the external environment with the primary function of delivering sperm cells to the female gametophyte. This study was conducted to identify proteins in tomato pollen and t
Publikováno v:
PROTEOMICS. 7:232-247
We have used 2-DE for a time-course study of the changes in protein and phosphoprotein expression that occur immediately after fertilization in Solanum chacoense. The phosphorylation status of the detected proteins was determined with three methods:
Publikováno v:
Sexual Plant Reproduction. 19:185-196
Proteome analysis of mature Arabidopsis thaliana (Landsberg erecta ecotype) pollen was conducted using two-dimensional gel electrophoresis and mass spectrometry. A total of 960 spots were resolved on pH 4–7 IPG strips and 110 distinct proteins were
Publikováno v:
PROTEOMICS. 5:3752-3764
Proteome analysis of embryo and endosperm tissues from germinating tomato seed was conducted using 1-DE, 2-DE, and MS. Mobilization of the most abundant proteins, which showed similar profiles in the two tissues, occurred first in the endosperm. CBB
Autor:
and Carmen Dereniwsky, Scott Napper, Jason Kindrachuk, Douglas J. H. Olson, Stephen J. Ambrose, Andrew R. S. Ross
Publikováno v:
Analytical Chemistry. 75:1741-1747
Phosphorylation is the predominant posttranslational modification involved in regulating enzymatic activity and mediating signal transduction in prokaryotic and eukaryotic cells. Selective enrichment of phosphorylated peptides prior to mass spectrome
Autor:
Rajendra K. Sharma, Shawn Ritchie, Mohammed Khysar Pasha, Andrew R. S. Ross, Danielle J. P. Batten, Keith Bonham, Douglas J. H. Olson
Publikováno v:
Nucleic Acids Research. 31:1502-1513
The human SRC gene encodes pp60(c-src), a non-receptor tyrosine kinase involved in numerous signaling pathways. Activation or overexpression of c-Src has also been linked to a number of important human cancers. Transcription of the SRC gene is comple
Publikováno v:
The FASEB Journal. 20:1555-1557
Elevated methylglyoxal (MG) levels have been reported in insulin-resistance syndrome. The present study investigated whether MG, a highly reactive metabolite of glucose, induced structural and functional changes of insulin. Incubation of human insuli
Publikováno v:
Canadian Journal of Chemistry. 74:238-245
Racemic abscisic acid (ABA), the cis and trans 1′, 4′-diols (ABA diols) derived from ABA by reduction of the 4′ ketone, and the corresponding 4′-O-acetates were converted into various acetosugar esters by reaction of their cesium salts with t