Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Douglas J. Cecchini"'
Publikováno v:
Natural Product Letters. 3:155-161
A sensitive bioassay for recombinant interleukin-1α (rIL-1α) has been developed. The assay is based on prostaglandin E2 (PGE2) production by fibroblasts following stimulation with rIL-1α. The stimulated PGE2 levels are determined by radioimmunoass
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8d51f617651243e13607fd6d20b92ab9
https://doi.org/10.1080/10575639308043854
https://doi.org/10.1080/10575639308043854
Autor:
Beverly A. Warden, Abdellah Sentissi, Kariman Alam, Roger W. Giese, Douglas J. Cecchini, Markus Ehrat
Publikováno v:
Journal of Immunological Methods. 131:77-82
Both the active ester and maleimide moieties of the cross-linking reagent, N-[(gamma-maleimidobutyryl)oxy]succinimide (GMBS), were found to react with the primary amino groups on ribonuclease (RNase). This largely inactivated RNase towards a polymeri
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::69d8e7521bdb591dd367637cd2ec3bfb
https://doi.org/10.1016/0022-1759(90)90235-n
https://doi.org/10.1016/0022-1759(90)90235-n
Publikováno v:
Journal of chromatography. 600(2)
A method has been developed in which the DNA of leukocytes (as the buffy coat from blood) is isolated in the form of its constituent deoxynucleotides. The steps in this method are as follows: (1) lyse the leukocytes with sodium dodecyl sulfate (SDS)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::acce3ea077ea57daf3d1682247b11f7a
https://pubmed.ncbi.nlm.nih.gov/1400847
https://pubmed.ncbi.nlm.nih.gov/1400847
Publikováno v:
Analytical Biochemistry. 162:171-177
A stable T-2 hydrazide gel is prepared by activating T-2 toxin with tresyl chloride followed by coupling to agarose-adipic acid hydrazide. Utilized as an affinity chromatography column, this T-2 hydrazide gel purifies a monoclonal antibody for T-2 in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::60dd3d28f4815bc02ab2a6fe8b2c0797
https://doi.org/10.1016/0003-2697(87)90024-8
https://doi.org/10.1016/0003-2697(87)90024-8
Publikováno v:
Steroids. 31:375-391
A radioimmunoassay method for urinary catechol estrogens is described; The specific nature of the antisera allows direct analyses of acid hydrolyzed urine. A LH-20 Sephadex column chromatography can be employed for individual determinations of 2-hydr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8558835c957496768a747fff98640328
https://doi.org/10.1016/0039-128x(78)90051-x
https://doi.org/10.1016/0039-128x(78)90051-x
Publikováno v:
Journal of Chromatography A. 326:311-320
Transamination conjugates of cytidine-3′-phosphate and polycytidylic acid (poly C) with diaminopropane, diaminohexane and diaminooctane (DAO) are formed both at 25°C and 60°C. The extent of reaction and formation of side products, with intermitte
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::71c1720940e4a4efd32f691537a9a7bc
https://doi.org/10.1016/s0021-9673(01)87456-2
https://doi.org/10.1016/s0021-9673(01)87456-2
Autor:
Herbert H. Wotiz, Aram V. Chobanian, Douglas J. Cecchini, Sati C. Chattoraj, Thomas F. Brennan, Kenneth C. Edelin
Publikováno v:
Steroids. 42:603-607
Since catechol estrogens are potent competitive inhibitors of catechol-O-methyl transferase (COMT), it has been suggested that they may prolong the half-life of catecholamines which in turn can cause hypertension. Thus, experiments were carried out t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b2a9c55880ffd8bb30fbd3b4e0a57ad
https://doi.org/10.1016/0039-128x(83)90123-x
https://doi.org/10.1016/0039-128x(83)90123-x
Publikováno v:
Clinical Chemistry. 32:1622-1630
The S-peptide and S-protein fragments of ribonuclease S (RNase S, no EC no. assigned) have been immobilized onto separate Sepharose gels via a "leash" of polycytidylic acid substrate. Each of these gels releases its RNase fragment when treated with t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c662d539693cc0511776b2956d13d67f
https://doi.org/10.1093/clinchem/32.9.1622
https://doi.org/10.1093/clinchem/32.9.1622
Autor:
Shymal K. Panda, Douglas J. Cecchini, Thomas F. Brennan, Jay S. Schinfeld, Gary L. Gross, Sati C. Chattoraj
Publikováno v:
Fertility and Sterility. 39:679-682
Catechol estrogens, estrogen metabolites of potential physiologic significance, were measured in infertile women undergoing ovulation induction with human menopausal gonadotropins. Urinary 2-hydroxyestrone (2-OH-E1) specimens were obtained from 12 wo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc70bb9c31f5660546543b367e18e204
https://doi.org/10.1016/s0015-0282(16)47065-2
https://doi.org/10.1016/s0015-0282(16)47065-2
Autor:
Adel S. Fanous, Sati C. Chattoraj, Kenneth C. Edelin, Thomas F. Brennan, Douglas J. Cecchini, Shyamal K. Panda
Publikováno v:
Endocrinology. 112(3)
A RIA for 2-hydroxyestrone (2-OHE1) in rat plasma has been developed. The assay employs an antiserum that is specific for catechol estrogens. Specificity is further ensured by purification of plasma extracts on Sephadex LH-20 columns before RIA. Bloo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::825e369eac3bf4417b751b170f7a880f
https://pubmed.ncbi.nlm.nih.gov/6401618
https://pubmed.ncbi.nlm.nih.gov/6401618