Zobrazeno 1 - 10
of 27
pro vyhledávání: '"Douglas F. Porter"'
Autor:
Douglas F. Porter, Weili Miao, Xue Yang, Grant A. Goda, Andrew L. Ji, Laura K. H. Donohue, Maria M. Aleman, Daniel Dominguez, Paul A. Khavari
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-16 (2021)
It is important to develop quantitative criteria to identify RNA-binding proteins and target RNAs. Here, the authors present a method to quantify cross-linking and apply it to recurrent cancer mutations in RNA-binding proteins.
Externí odkaz:
https://doaj.org/article/dd96eec6ff1a4e01a51eb5bbd511fef7
Autor:
Douglas F. Porter, Aman Prasad, Brian H. Carrick, Peggy Kroll-Connor, Marvin Wickens, Judith Kimble
Publikováno v:
G3: Genes, Genomes, Genetics, Vol 9, Iss 1, Pp 153-165 (2019)
Metazoan PUF (Pumilio and FBF) RNA-binding proteins regulate various biological processes, but a common theme across phylogeny is stem cell regulation. In Caenorhabditis elegans, FBF (fem-3 Binding Factor) maintains germline stem cells regardless of
Externí odkaz:
https://doaj.org/article/4199407a06c44f8ea331e1f7e7f77bb0
Autor:
Scott T. Aoki, Douglas F. Porter, Aman Prasad, Marvin Wickens, Craig A. Bingman, Judith Kimble
Publikováno v:
Cell Reports, Vol 23, Iss 13, Pp 3769-3775 (2018)
Summary: FOG-3 is a master regulator of sperm fate in Caenorhabditis elegans and homologous to Tob/BTG proteins, which in mammals are monomeric adaptors that recruit enzymes to RNA binding proteins. Here, we determine the FOG-3 crystal structure and
Externí odkaz:
https://doaj.org/article/f317025531f3428a9fa1c9ecfd373326
Publikováno v:
Journal of proteome research. 21(8)
Kinases play important roles in cell signaling, and adenosine monophosphate (AMP) is known to modulate cellular energy homeostasis through AMP-activated protein kinase (AMPK). Here, we explored novel AMP-binding kinases by employing a desthiobiotin-c
Autor:
Smarajit Mondal, Muthukumar Ramanathan, Weili Miao, Robin M. Meyers, Deepti Rao, Vanessa Lopez-Pajares, Zurab Siprashvili, David L. Reynolds, Douglas F. Porter, Ian Ferguson, Poornima Neela, Yang Zhao, Lindsey M. Meservey, Margaret Guo, Yen-Yu Yang, Lin Li, Yinsheng Wang, Paul A. Khavari
Publikováno v:
Nat Methods
DNA-protein interactions mediate physiologic gene regulation and may be altered by DNA variants linked to polygenic disease. To enhance the speed and signal-to-noise ratio (SNR) in the identification and quantification of proteins associated with spe
Autor:
Douglas F. Porter, Raghav M. Garg, Robin M. Meyers, Weili Miao, Luca Ducoli, Brian J. Zarnegar, Paul A. Khavari
Publikováno v:
Current Protocols. 3
Autor:
Weili Miao, Douglas F. Porter, Vanessa Lopez-Pajares, Zurab Siprashvili, Robin M. Meyers, Yunhao Bai, Duy T. Nguyen, Lisa A. Ko, Brian J. Zarnegar, Ian D. Ferguson, Matthew M. Mills, Christie E. Jilly-Rehak, Cheng-Guo Wu, Yen-Yu Yang, Jordan M. Meyers, Audrey W. Hong, David L. Reynolds, Muthukumar Ramanathan, Shiying Tao, Sizun Jiang, Ryan A. Flynn, Yinsheng Wang, Garry P. Nolan, Paul A. Khavari
Publikováno v:
Cell. 186(1)
Glucose is a universal bioenergy source; however, its role in controlling protein interactions is unappreciated, as are its actions during differentiation-associated intracellular glucose elevation. Azido-glucose click chemistry identified glucose bi
Autor:
Douglas F Porter, Raghav M Garg, Robin M. Meyers, Weili Miao, Luca Ducoli, Brian J Zarnegar, Paul A Khavari
The easyCLIP protocol describes a method for both normal CLIP library construction and the absolute quantification of RNA cross-linking rates, data which could be usefully combined to analyze RNA-protein interactions. Using these cross-linking metric
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0358c9b3bd76ad08e9cf65f6a6e7d2ad
https://doi.org/10.1101/2021.12.15.472862
https://doi.org/10.1101/2021.12.15.472862
Autor:
Christopher P. Lapointe, Melanie A. Preston, Judith Kimble, Natascha Buter, Sunduz Keles, Marvin Wickens, Fan Chen, Douglas F. Porter
Publikováno v:
Nature methods
Ribonucleotidyl transferases (rNTases) add untemplated ribonucleotides to diverse RNAs. We have developed TRAID-seq, a screening strategy in Saccharomyces cerevisiae to identify sequences added to a reporter RNA at single-nucleotide resolution by ove
Publikováno v:
Nature Methods. 16:225-234
Noncoding RNA sequences, including long noncoding RNAs, small nucleolar RNAs, and untranslated mRNA regions, accomplish many of their diverse functions through direct interactions with RNA-binding proteins (RBPs). Recent efforts have identified hundr