Zobrazeno 1 - 7
of 7
pro vyhledávání: '"Douglas Cossar"'
Publikováno v:
The Canadian Journal of Chemical Engineering. 89:879-888
A metabolic reaction network including carbohydrate and amino acid metabolism in both anabolic and catabolic reactions was developed for Streptomyces lividans. Based on observed nutrient consumption, the fermentation process comprised two stages: ami
Autor:
Douglas Cossar, Andrew P. Laws, Timothy Miller, Ammar AL-Jasim, Mark Davis, Paul N. Humphreys
Publikováno v:
Carbohydrate research. 421
NMR and anion exchange chromatography analysis of the waste streams generated during the commercial production of sophorolipids by the yeast Candida bombicola identified the presence of small but significant quantities (1% w/v) of free sophorose. Sop
Autor:
Vadim Tsvetnitsky, Craig Kearse, David Miller, Douglas Cossar, Sally Xueying Ni, Adrian Man, Karen Norek
Publikováno v:
Protein Expression and Purification. 30:62-68
Recombinant Streptomyces griseus aminopeptidase (SGAP) was produced using Cangene’s expression system, CANGENUS. This heat-stable aminopeptidase with an N-terminal Ala-Pro-Asp-Ile-Pro-Leu-Ala-Asn-Val-Lys-Ala sequence was purified from 16 L of Strep
Autor:
Douglas Cossar, Donald I. H. Stewart, Phyllis Krygsman, Dan Trudeau, Alexander Szabo, Craig Binnie, David L. Jenish, Lawrence T. Malek
Publikováno v:
Protein Expression and Purification. 11:271-278
A gene encoding the extracellular domain of the human erythropoietin receptor (EPO-R) was constructed using oligonucleotides, with a view to maintaining preferred codon usage for the Streptomycetes. The gene was subcloned into a multicopy Streptomyce
Publikováno v:
Trends in biotechnology. 15(8)
The commercial production of human proteins in recombinant microorganisms for therapeutic use is well established. Systems have been developed to exploit the natural ability of certain bacteria to secrete properly folded, bioactive proteins into the
Autor:
Giorgio Canevascini, Douglas Cossar
Publikováno v:
Applied Microbiology and Biotechnology. 24
The cellulolytic fungus Sporotrichum (Chrysosporium) thermophile produces an extracellular cellobiose dehydrogenase during batch culture on cellulose or cellobiose. In chemostat culture at pH 5.6 on cellobiose this enzyme was produced in parallel wit
Publikováno v:
In Trends in Biotechnology 1997 15(8):315-320