Development and characterization of a novel ELISA based assay for the quantitation of sub-nanomolar levels of neoepitope exposed NITEGE-containing aggrecan fragments

Autor: Kevin L. Duffin, Craig A. Swearingen, Dotzlaf Joe Edward, Kannan Thirunavukkarasu, Mark Chambers, Quincy L. Carter, P.G. Mitchell, I.J. Brittain

Publikováno v: Journal of Immunological Methods. 328:162-168

Osteoarthritis (OA) is associated with the degradation of aggrecan by aggrecanases (e.g. ADAMTS-4, ADAMTS-5) ultimately leading to the reduction of daily physical activity in aged individuals. The cleavage of aggrecan by aggrecanases generates a seri

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e06280dbcf9bb50aff258ba76feb7862
https://doi.org/10.1016/j.jim.2007.08.018

Cloning and characterization of femA and femB from Staphylococcus epidermidis

Autor: Wu-Kuang Yeh, Serhat Ünal, Dotzlaf Joe Edward, Paul L. Skatrud, Carolyn A. Hayes, J. E. Flokowitsch, JoAnn Hoskins, William E. Alborn

Publikováno v: Gene. 180:177-181

A DNA fragment was identified and cloned from Staphylococcus epidermidis (Se) using femA from S. aureus (Sa) as a heterologous hybridization probe. DNA sequence analysis of a portion of this clone revealed two complete ORFs highly related to femA and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ee9b4da5cf4c29329055778e3fab43e6
https://doi.org/10.1016/s0378-1119(96)00450-7

Purification, properties, and kinetics of enzymatic acylation with beta-lactams of soluble penicillin-binding protein 2a. A major factor in methicillin-resistant Staphylococcus aureus

Autor: Dotzlaf Joe Edward, Wu-Kuang Yeh, Ghag Sandhya K, Siddhartha Roychoudhury

Publikováno v: Journal of Biological Chemistry. 269:12067-12073

Intrinsic resistance toward beta-lactams in methicillin-resistant Staphylococcus aureus strains, a major source of nosocomial infections, is believed to be caused mainly by penicillin-binding protein 2a (PBP2a). This protein resembles other penicilli

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::cf1f023ed33ed14c43ae47b9035ed7e0
https://doi.org/10.1016/s0021-9258(17)32681-9

A global benchmark study using affinity-based biosensors

Autor: Aykut Üren, Stephen G. Brohawn, Eva Muñoz, Kenneth Miller, Mike Scott, Heather Hughes, Yasmina Noubia Abdiche, Rohn Lee Millican, Anne W Emerick, Jonathan Brooks, Michael B. Murphy, Charlene S. Lee, Carmelo Di Primo, Joshua Ballard, Quyhn Trinh, Giuseppe A. Papalia, Cynthia S. Hinck, Kevin Thompson, Peter Flynn, Joshua S. Klein, Martin A. Wear, Mark Toews, Richard N. Bohnsack, Mohammed Yousef, Daniel Malashock, Gaetano Barbato, Jill Raymond, Alison Joyce, Min Hsiang Yang, Julie Rothacker, Patrick England, Ashique Rafique, Jacinto López-Sagaseta, Scott L. Klakamp, Daulet Satpaev, Monica Dines, Danny Terwey, Islay Campbell, Joshua Thompson, Ben Busby, Tanmoy Ganguly, Gerardo Gutierrez-Sanchez, Sergei Shikov, Kevin Lindquist, Momchilo Vuyisich, Asya Grinberg, Krista Witte, Yun Hee Cho, Yue Ji Li, Hubert Mantz, Ewa Pol, Gonzalo Obal, Brian J. Pak, María J. Hernáiz, Peter Kainz, Erk Gedig, Mary M. Murphy, Tsafir Bravman, Janus Krarup, Gabrielle Zeder-Lutz, John Quinn, Thomas E. Ryan, Pietro Brandani, Dawn Kernaghan, Anca Clabbers, M. Brent Waddell, Agnes Puskas, Petr Skládal, Sanjay Nilapwar, Kara Herlihy, Gregor Anderluh, Federico Torta, Eric Hommema, Andrej Bavdek, Rejane Guimaraes, Anthony M. Giannetti, Eric Fang, Henrik Anderson, Anne Birgitte Bagge Hagel, Schuyler B Corry, Mark R. Witmer, Phillippe Neuner, Sébastien Wieckowski, Nico Dankbar, Alanna Pinkerton, Suparna Mundodo, Trevor D. Chapman, Sylvie Canepa, Satya P. Yadav, Rostislav Skrabana, Oleksandr Kalyuzhniy, Phini S Katsamba, Vidya Chandrasekaran, Eugene G. Chomey, Dana Reichmann, Katy McGirr, Dotzlaf Joe Edward, Mireille Baltzinger, Ite A. Laird-Offringa, Liann Wang, Erica Boni, Tiffany Tsang, Zaneta Nikolovska-Coleska, Andreas Schoenemann, Yuki Abe, Jamie Furneisen, Kenneth T. Lewis, Eileen M. Lafer, John Corbin, Satyen Gautam, Yuguo Feng, Olan Dolezal, Sylviane Hoos, James R. Horn, Bianca Beusink, Jinlin Peng, Otto Pritsch, Kristian H. Schlick, Ryan James Darling, Malgorzata Mikolajczyk, Quincy L. Carter, Jason T. Schuman, Yang Liu, Abdelkrim Khadir, Loïc Martin, Mark Alan Lewis, Ganeshram Krishnamoorthy, Andrew W. Drake, Jason Baardsnes, George Korza, Jesper Pass, Judie Berlier, Melicia Gainey, Nguyen Ly, James R. Partridge, Rosy Calvert, Roberta D'Agata, Rebecca L. Rich, Monica E. Ferreira, Phillip Page, Frank John Podlaski, Jay Duffner, Ruchira Das Gupta, Melanie Wong, Sergei Bibikov, Jiejin Li, Paola Torreri, Bruce A. Andrien, Peter Spies, Christina Boozer, David G. Myszka

Publikováno v: Analytical Biochemistry
Analytical Biochemistry, Elsevier Masson, 2009, 386 (2), pp.194-216. ⟨10.1016/j.ab.2008.11.021⟩
Analytical biochemistry, 386(2):10.1016/j.ab.2008.11.021, 194-216. Academic Press Inc.
Analytical Biochemistry, 2009, 386 (2), pp.194-216. ⟨10.1016/j.ab.2008.11.021⟩
Rich, R L, Papalia, G A, Flynn, P J, Furneisen, J, Quinn, J, Klein, J S, Katsamba, P S, Waddell, M B, Scott, M, Thompson, J, Berlier, J, Corry, S, Baltzinger, M, Zeder-Lutz, G, Schoenemann, A, Clabbers, A, Wieckowski, S, Murphy, M M, Page, P, Ryan, T E, Duffner, J, Ganguly, T, Corbin, J, Gautam, S, Anderluh, G, Bavdek, A, Reichmann, D, Yadav, S P, Hommema, E, Pol, E, Drake, A, Klakamp, S, Chapman, T, Kernaghan, D, Miller, K, Schuman, J, Lindquist, K, Herlihy, K, Murphy, M B, Bohnsack, R, Andrien, B, Brandani, P, Terwey, D, Millican, R, Darling, R J, Wang, L, Carter, Q, Dotzlaf, J, Lopez-Sagaseta, J, Campbell, I, Torreri, P, Hoos, S, England, P, Liu, Y, Abdiche, Y, Malashock, D, Pinkerton, A, Wong, M, Lafer, E, Hinck, C, Thompson, K, Primo, C D, Joyce, A, Brooks, J, Torta, F, Bagge Hagel, A B, Krarup, J, Pass, J, Ferreira, M, Shikov, S, Mikolajczyk, M, Abe, Y, Barbato, G, Giannetti, A M, Krishnamoorthy, G, Beusink, B, Satpaev, D, Tsang, T, Fang, E, Partridge, J, Brohawn, S, Horn, J, Pritsch, O, Obal, G, Nilapwar, S, Busby, B, Gutierrez-Sanchez, G, Gupta, R D, Canepa, S, Witte, K, Nikolovska-Coleska, Z, Cho, Y H, D'Agata, R, Schlick, K, Calvert, R, Munoz, E M, Hernaiz, M J, Bravman, T, Dines, M, Yang, M-H, Puskas, A, Boni, E, Li, J, Wear, M, Grinberg, A, Baardsnes, J, Dolezal, O, Gainey, M, Anderson, H, Peng, J, Lewis, M, Spies, P, Trinh, Q, Bibikov, S, Raymond, J, Yousef, M, Chandrasekaran, V, Feng, Y, Emerick, A, Mundodo, S, Guimaraes, R, McGirr, K, Li, Y-J, Hughes, H, Mantz, H, Skrabana, R, Witmer, M, Ballard, J, Martin, L, Skladal, P, Korza, G, Laird-Offringa, I, Lee, C S, Khadir, A, Podlaski, F, Neuner, P, Rothacker, J, Rafique, A, Dankbar, N, Kainz, P, Gedig, E, Vuyisich, M, Boozer, C, Ly, N, Toews, M, Uren, A, Kalyuzhniy, O, Lewis, K, Chomey, E, Pak, B J & Myszka, D G 2009, ' A global benchmark study using affinity-based biosensors ', Analytical Biochemistry, vol. 386, no. 2, pp. 194-216 . https://doi.org/10.1016/j.ab.2008.11.021

International audience; To explore the variability in biosensor studies, 150 participants from 20 countries were given the same protein samples and asked to determine kinetic rate constants for the interaction. We chose a protein system that was amen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::15f0511d7d5e8ed6e3424eec0c582e12
https://hal.archives-ouvertes.fr/hal-01527917

Enzymatic synthesis of diastereospecific carbacephem intermediates using serine hydroxymethyltransferase

Autor: Jeffrey Thomas Vicenzi, Dotzlaf Joe Edward, A J Kreuzman, Joseph Martin Zock, Wu-Kuang Yeh, Stephen Wyatt Queener

Publikováno v: Journal of industrial microbiologybiotechnology. 19(5-6)

The serine hydroxymethyltransferase (SHMT) gene glyA was over-expressed in Escherichia coli and the enzyme was purified to near homogeneity. Reaction conditions for E. coli and rabbit liver SHMTs were optimized using succinic semialdehyde methyl este

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a403ee48cce526da05a4adfb94a8fccc
https://pubmed.ncbi.nlm.nih.gov/9451834