Zobrazeno 1 - 10
of 19
pro vyhledávání: '"Dorotea Fracchiolla"'
Autor:
Dorotea Fracchiolla, Justyna Sawa-Makarska, Bettina Zens, Anita de Ruiter, Gabriele Zaffagnini, Andrea Brezovich, Julia Romanov, Kathrin Runggatscher, Claudine Kraft, Bojan Zagrovic, Sascha Martens
Publikováno v:
eLife, Vol 5 (2016)
Selective autophagy is mediated by cargo receptors that link the cargo to the isolation membrane via interactions with Atg8 proteins. Atg8 proteins are localized to the membrane in an ubiquitin-like conjugation reaction, but how this conjugation is c
Externí odkaz:
https://doaj.org/article/9e792a1fdaea4938bbb493df72516559
Autor:
Bettina Wurzer, Gabriele Zaffagnini, Dorotea Fracchiolla, Eleonora Turco, Christine Abert, Julia Romanov, Sascha Martens
Publikováno v:
eLife, Vol 4 (2015)
Autophagy is a major pathway for the clearance of harmful material from the cytoplasm. During autophagy, cytoplasmic material is delivered into the lysosomal system by organelles called autophagosomes. Autophagosomes form in a de novo manner and, in
Externí odkaz:
https://doaj.org/article/9d78664c88bf4961a3a8a86bba5854e8
Autor:
Marina N. Iriondo, Asier Etxaniz, Yaiza R. Varela, Uxue Ballesteros, Melisa Lázaro, Mikel Valle, Dorotea Fracchiolla, Sascha Martens, L. Ruth Montes, Félix M. Goñi, Alicia Alonso
Publikováno v:
Cellular and Molecular Life Sciences. 80
In macroautophagy, the autophagosome (AP) engulfs portions of cytoplasm to allow their lysosomal degradation. AP formation in humans requires the concerted action of the ATG12 and LC3/GABARAP conjugation systems. The ATG12–ATG5-ATG16L1 or E3-like c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3ebe3c3a9bf68b3713bfda0fb20744fc
https://doi.org/10.1007/s00018-023-04704-z
https://doi.org/10.1007/s00018-023-04704-z
Autor:
Marina N. Iriondo, Asier Etxaniz, Yaiza R. Varela, Uxue Ballesteros, Melisa Lázaro, Mikel Valle, Dorotea Fracchiolla, Sascha Martens, L. Ruth Montes, Felix M Goni, Alicia Alonso
In macroautophagy, the autophagosome (AP) engulfs portions of cytoplasm to allow their lysosomal degradation. AP formation in humans requires the concerted action of the ATG12 and LC3/GABARAP conjugation systems. The ATG12–ATG5-ATG16L1 (E3) complex
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4fd5d35e52dd7a1600133a626d9686e5
https://doi.org/10.21203/rs.3.rs-1925210/v1
https://doi.org/10.21203/rs.3.rs-1925210/v1
Autor:
Thanh Ngoc Nguyen, Justyna Sawa-Makarska, Grace Khuu, Wai Kit Lam, Elias Adriaenssens, Dorotea Fracchiolla, Stephen Shoebridge, Benjamin Scott Padman, Marvin Skulsuppaisarn, Runa S.J. Lindblom, Sascha Martens, Michael Lazarou
Cargo sequestration is a fundamental step of selective autophagy in which cells generate a double membrane structure termed an autophagosome on the surface of cargoes. NDP52, TAX1BP1 and p62 bind FIP200 which recruits the ULK1/2 complex to initiate a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::72f5f7926227cbd10af1584155a1be5b
https://doi.org/10.1101/2022.08.14.503930
https://doi.org/10.1101/2022.08.14.503930
Autor:
Dorotea Fracchiolla
This protocol describes the procedures for expression and purification of the active autophagy-specific human PI3KC3-C1 kinase complex.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::dc9656440bcf8c126aadee6893c0ece8
https://doi.org/10.17504/protocols.io.8epv59mz4g1b/v1
https://doi.org/10.17504/protocols.io.8epv59mz4g1b/v1
Publikováno v:
Journal of Molecular Biology
Autophagy is a major cellular degradation pathway, which mediates the delivery of cytoplasmic cargo material into lysosomes. This is achieved by the specific sequestration of the cargo within double-membrane vesicles, the autophagosomes, which form d
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::db7a04a4f82f70dc691a18e2db276763
https://doi.org/10.1016/j.jmb.2019.07.027
https://doi.org/10.1016/j.jmb.2019.07.027
Autor:
Dorotea Fracchiolla
This protocol describes expression and purification procedures for obtaining mCherry-tagged human recombinant Ubiquitin-like modifier LC3B (MAP1LC3B, Microtubule-associated proteins 1A/1B light chain 3B) lacking five C-terminal amino acids to allow i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1ed64fb288be29011a9e03704b845033
https://doi.org/10.17504/protocols.io.btiunkew
https://doi.org/10.17504/protocols.io.btiunkew
Autor:
Dorotea Fracchiolla
This protocol describes expression and purification procedures for obtaining GST-tagged human recombinant Autophagy specific de-ubiquitinase ATG4B (AuTophaGy-related protein).
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::77080a6e383b883c60f066f9403fd5d5
https://doi.org/10.17504/protocols.io.bseanbae
https://doi.org/10.17504/protocols.io.bseanbae
Autor:
Liv E. Jensen, Chunmei Chang, Dorotea Fracchiolla, Abraham King Cada, Sascha Martens, James H. Hurley
Publikováno v:
Biophysical Journal. 121:82a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::98cb26831efc8f30fad957c88e5f79ef
https://doi.org/10.1016/j.bpj.2021.11.2330
https://doi.org/10.1016/j.bpj.2021.11.2330