Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Doreen Ackermann"'
Autor:
Martin Busch, Kira Leona Wefelmeyer, Karoline Walscheid, Kai Rothaus, Dirk Bauer, Cornelia A. Deeg, Roxane L. Degroote, Doreen Ackermann, Simone König, Solon Thanos, Maren Kasper, Arnd Heiligenhaus
Publikováno v:
Frontiers in Immunology, Vol 10 (2019)
The purpose of the current study was to analyze the binding patterns of serum autoantibodies from juvenile idiopathic arthritis (JIA) and JIA-associated uveitis (JIAU) patients to proteomes from different ocular tissues and to identify potential ocul
Externí odkaz:
https://doaj.org/article/34da7bd2f2334957abf8ad6301015d7f
Publikováno v:
PLoS ONE, Vol 6, Iss 3, p e18098 (2011)
BACKGROUND: Differential gel electrophoresis (DIGE) is a technology widely used for protein expression analysis. It is based on labelling with fluorescent Cy dyes. In comparative fluorescence gel electrophoresis experiments, however, unspecific label
Externí odkaz:
https://doaj.org/article/8d9ae0a7272f4453999e8c5da1553d62
Autor:
Doreen Ackermann, Simone König
Publikováno v:
Methods in Molecular Biology ISBN: 9781071628300
Methods in Molecular Biology ISBN: 9781493972678
Methods in Molecular Biology ISBN: 9781493972678
Two-dimensional comparative fluorescence gel electrophoresis (CoFGE) uses an internal standard to increase the reproducibility of coordinate assignment for protein spots visualized on 2D polyacrylamide gels. This is particularly important for samples
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0e10de9190de21abf475a691bb5adafd
https://doi.org/10.1007/978-1-0716-2831-7_5
https://doi.org/10.1007/978-1-0716-2831-7_5
Autor:
Johannes A. Eble, Maryam Rezaei, Doreen Ackermann, Simone König, Isabel Siepe, Juliane Meißner
Publikováno v:
Free Radical Biology and Medicine. 164:341-353
Proteomics studies have revealed that adhesomes are assembled from a plethora of proteins at integrin-mediated cellular contact sites with the extracellular matrix. By combining dimedone-trapping of sulfenylated proteins with the purification of the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::000bbbf7ee8a48a05ac0abe9b5be2321
https://doi.org/10.1016/j.freeradbiomed.2021.01.019
https://doi.org/10.1016/j.freeradbiomed.2021.01.019
Autor:
Dirk Bauer, Doreen Ackermann, Karoline Walscheid, Monika Stoll, Christoph Tappeiner, Anika Witten, Arnd Heiligenhaus, Harutyun Melkonyan, Lena Wildschütz, Simone König, Carsten Heinz, J M Koch, Björn Laffer, Solon Thanos, Andrei Barysenka, Martin Busch, Maren Kasper, Shirin Glander
Gene and protein expression profiles of iris biopsies, aqueous humor (AqH), and sera in patients with juvenile idiopathic arthritis-associated uveitis (JIAU) in comparison to control patients with primary open-angle glaucoma (POAG) and HLA-B27-positi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::efd60fed1fdb1e6e69e12a10ccc71f1c
Autor:
Simone König, Doreen Ackermann
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1664
Comparative two-dimensional gel electrophoresis (CoFGE) is a special version of two-dimensional polyacrylamide GE (2D-PAGE) and related to difference GE (2D-DIGE). It provides reproducibility and standardisation for 2D-PAGE by introducing a reference
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::54df586f7f15e551120c67faa97606f8
https://pubmed.ncbi.nlm.nih.gov/29019125
https://pubmed.ncbi.nlm.nih.gov/29019125
Publikováno v:
ELECTROPHORESIS. 33:1406-1410
The comparison of proteins separated on 2DE is difficult due to gel-to-gel variability. Here, a method named comparative fluorescence gel electrophoresis (CoFGE) is presented, which allows the generation of an artificial protein grid in parallel to t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::50fd29ec92ab0c22453546ee8bb02014
https://doi.org/10.1002/elps.201200039
https://doi.org/10.1002/elps.201200039
Publikováno v:
BIOspektrum. 20:655-657
A major problem in two-dimensional protein gel electrophoresis is the little comparability of gel images. A reference grid of marker proteins in parallel to the separation of the analyte by use of two fluorescent dyes allows the correction of protein
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::68c4dc519b2ac336eb52c9bf4e6ed989
https://doi.org/10.1007/s12268-014-0503-5
https://doi.org/10.1007/s12268-014-0503-5
Publikováno v:
PROTEOMICS. 10:1525-1529
In comparative fluorescence gel electrophoresis experiments, cross-talk was detected. It was traced back to a failure in the quenching process in typical labelling protocols. Despite a huge excess of potential reaction sites for the N-hydroxy-succini
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2fb889f7c4bf470bf7777d823890f14e
https://doi.org/10.1002/pmic.200900679
https://doi.org/10.1002/pmic.200900679
Publikováno v:
EuPA Open Proteomics. 5
Here we present the evaluation of the newly developed impact II QTOF mass spectrometer (Bruker Daltonics, Germany). The capability to acquire high quality MS/MS data with more than 40Hz resulted in a significant increase in protein identification. To
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6ab2aac83209de9199cf97b845b86bd3