Zobrazeno 1 - 10
of 43
pro vyhledávání: '"Doreen A. Wüstenhagen"'
Autor:
Jan Felix Knauer, Christian Schulz, Anne Zemella, Doreen A. Wüstenhagen, Ruben Magnus Walter, Jan-Heiner Küpper, Stefan Kubick
Publikováno v:
Scientific Reports, Vol 14, Iss 1, Pp 1-13 (2024)
Abstract Cytochromes P450 (CYPs) are a group of monooxygenases that can be found in almost all kinds of organisms. For CYPs to receive electrons from co-substrate NADPH, the activity of NADPH-Cytochrome-P450-oxidoreductase (CPR) is required as well.
Externí odkaz:
https://doaj.org/article/54ec96165b0e49408cbc32198fc8fced
Autor:
Simon K. Krebs, Marlitt Stech, Felix Jorde, Nathanaël Rakotoarinoro, Franziska Ramm, Sophie Marinoff, Sven Bahrke, Antje Danielczyk, Doreen A. Wüstenhagen, Stefan Kubick
Publikováno v:
International Journal of Molecular Sciences, Vol 23, Iss 22, p 13697 (2022)
Recombinant immunotoxins (RITs) are an effective class of agents for targeted therapy in cancer treatment. In this article, we demonstrate the straight-forward production and testing of an anti-CD7 RIT based on PE24 in a prokaryotic and a eukaryotic
Externí odkaz:
https://doaj.org/article/5690bafebb7840d7809634ffa615c64c
Autor:
Srujan Kumar Dondapati, Henning Lübberding, Anne Zemella, Lena Thoring, Doreen A. Wüstenhagen, Stefan Kubick
Publikováno v:
Frontiers in Pharmacology, Vol 10 (2019)
Cell-free protein synthesis (CFPS) based on eukaryotic Sf21 lysate is gaining interest among researchers due to its ability to handle the synthesis of complex human membrane proteins (MPs). Additionally Sf21 cell-free systems contain endogenous micro
Externí odkaz:
https://doaj.org/article/39ec09c8196144baa1d5b3d3ba3ae5d1
Publikováno v:
PLoS ONE, Vol 14, Iss 4, p e0215897 (2019)
The Toll-like receptor family belongs to the group of pathogen recognition receptors which is responsible for the discrimination of self and non-self pathogen-associated molecular patterns (PAMP's). Toll-like receptors play an important role in the i
Externí odkaz:
https://doaj.org/article/de3f9feb9b074b1b910a3383494dc8e6
Autor:
Stefan Mergler, Srujan Kumar Dondapati, Dennis Bräuer, Stefan Kubick, Doreen A. Wüstenhagen, Priyavathi Dhandapani, Anne Zemella
Publikováno v:
RSC Advances. 11:16285-16296
Calcium imaging is an important functional tool for analysing ion channels, transporters and pumps for drug screening in living cells. Depicted eukaryotic cell-free systems utilize microsomes, derived from the endoplasmic reticulum to incorporate the
Autor:
Doreen A, Wüstenhagen, Phil, Lukas, Christian, Müller, Simone A, Aubele, Jan-Peter, Hildebrandt, Stefan, Kubick
Publikováno v:
Scientific Reports, Vol 10, Iss 1, Pp 1-13 (2020)
Scientific Reports
Scientific Reports
Synthesis and purification of peptide drugs for medical applications is a challenging task. The leech-derived factor hirudin is in clinical use as an alternative to heparin in anticoagulatory therapies. So far, recombinant hirudin is mainly produced
Autor:
Lena Thoring, Doreen A Wüstenhagen, Maria Borowiak, Marlitt Stech, Andrei Sonnabend, Stefan Kubick
Publikováno v:
PLoS ONE, Vol 11, Iss 9, p e0163670 (2016)
Nowadays, biotechnological processes play a pivotal role in target protein production. In this context, Chinese Hamster Ovary (CHO) cells are one of the most prominent cell lines for the expression of recombinant proteins and revealed as a safe host
Externí odkaz:
https://doaj.org/article/1b05bfc4ebda4570b27df2b98e320a15
Autor:
Silke Bechlars, Claudia Jäckel, Susanne Diescher, Doreen A Wüstenhagen, Stefan Kubick, Ralf Dieckmann, Eckhard Strauch
Publikováno v:
PLoS ONE, Vol 10, Iss 3, p e0118559 (2015)
Vibrio parahaemolyticus is a recognized human enteropathogen. Thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH) as well as the type III secretion system 2 (T3SS2) are considered as major virulence factors. As tdh positive strains ar
Externí odkaz:
https://doaj.org/article/f56d4630f5a64f7a80dfa952f2f55fdb
Cell-free production is a valuable and alternative method for the synthesis of membrane proteins. This system offers openness allowing the researchers to modify the reaction conditions without any boundaries. Additionally, the cell-free reactions are
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7e650afafcf8eb33bd674248f5a18f34
https://doi.org/10.17504/protocols.io.bpsqmndw
https://doi.org/10.17504/protocols.io.bpsqmndw
Autor:
Doreen A. Wüstenhagen, Dennis Bräuer, Stefan Kubick, Srujan Kumar Dondapati, Anne Zemella, Priyavathi Dhandapani
Calcium imaging is an important functional tool for addressing ion channels, transporters and pumps for drug screening in living cells. Depicted eukaryotic cell-free systems utilize microsomes, derived from endoplasmic reticulum to incorporate the sy
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a3d137825994ffaf07f8a10b3fff104d