Zobrazeno 1 - 10
of 18
pro vyhledávání: '"Donogh P. O'Brien"'
Autor:
Catherine Lecoffre, Donogh P. O’Brien, Christiane Gauffeny, Catherine Lunven, Nigel O. Roome, Christopher N. Berry
Publikováno v:
Thrombosis and Haemostasis. 75:154-169
SummaryClot-associated thrombin retains amidolytic activity, and is resistant to inhibition by heparin, but not to low molecular weight thrombin inhibitors. We show that clot-associated thrombin induces platelet aggregation, is resistant to heparin:a
Autor:
Donogh P. O'Brien, John H. McVey, Edward G. D. Tuddenham, Ian Garner, Yasufumi Imanaka, Geoffrey Kemball-Cook, Takuya Nishimura
Publikováno v:
Gene. 139:275-279
Recombinant human proteins are generally recovered in low yields from mammalian tissue culture following transfection with commercially available vectors. We have constructed a novel vector containing both the neomycin-resistance-encoding gene (neo)
Publikováno v:
Biochemistry. 32:13949-13955
Synthetic peptides corresponding to portions of the wild-type and variant sequences of the human factor VII gamma-carboxyglutamic acid (Gla)-containing domain have been prepared by direct peptide synthesis using the Fmoc-based protection strategy. Pe
Publikováno v:
Biochemistry. 31:2805-2812
Factor VIII (FVIII) is the nonproteolytic cofactor for FIXa in the tenase complex of blood coagulation. FVIII is proteolytically activated by thrombin and FXa in vitro to form a heterotrimer with full procoagulant activity. Activated protein C inacti
Autor:
JS Anderson, Egd Tuddenham, John H. McVey, Karen M. Gale, Donogh P. O'Brien, George J. Miller, TW Meade
Publikováno v:
Blood. 78:132-140
Factor VII (FVII) is the plasma serine protease zymogen which, on binding to its cellular receptor tissue factor (TF), initiates blood coagulation. A 47-year-old man with no clinical bleeding tendency was found to have undetectable plasma FVII activi
Publikováno v:
Blood. 75:1664-1672
We have purified factor VIII from a patient with moderately severe hemophilia A (FVIII, 4 U/dL; FVIII:Ag, 110 U/dL) and subjected the protein to Western blot analysis after time course activation with thrombin. The cross reacting material-positive (C
Autor:
Christine Girardot, Patrice Ferrari, Marie-Christine Charles, Irène Lechaire, Catherine Lunven, Donogh P. O’Brien, Christopher N. Berry, Denise Girard
Publikováno v:
Thrombosis and haemostasis. 79(1)
SummaryWe studied the use of the Ecarin Clotting Time (ECT) as a predictive assay of the antithrombotic effects of argatroban in a new tissue factor-dependent model of venous thrombosis and a model of arterial thrombosis in the rat. Heparin was used
Autor:
Alun W. Ashton, Daniel J.D. Johnson, David M. A. Martin, Stephen J. Perkins, Edward G. D. Tuddenham, Donogh P. O'Brien, Geoffrey Kemball-Cook
Publikováno v:
FEBS letters. 374(1)
The four-domain structure of human factor VIIa and the two-domain structure of tissue factor form a tight complex to initiate blood coagulation. By solution scattering, the mean X-ray and neutron radii of gyration RG (which determine macromolecular e
Autor:
David M. A. Martin, Karl Harlos, Donogh P. O'Brien, C.W.G. Boys, Edward G. D. Tuddenham, Andrew P. Miller
Publikováno v:
Journal of molecular biology. 234(4)
The extracellular domain (residues 1 to 220) of human tissue factor has been cloned and expressed in Escherichia coli and purified to isoelectric homogeneity. Single crystals suitable for X-ray analysis have been obtained by vapour diffusion. They be
Autor:
Andrew P. Miller, E Y Jones, Karl Harlos, Igor Polikarpov, Boys Cwg., Donogh P. O'Brien, David I. Stuart, Tuddenham Egd., Martin Dma.
Publikováno v:
Nature. 371:720-720
Nature 370, 662-666 (1994) THE last line of the Acknowledgements section was omitted from this Letter. This read: "C. W. G. Boys was supported by the Wellcome Trust."