Best practices for performance of real-time PCR assays in veterinary diagnostic laboratories

Autor: Roman Pogranichniy, Kathy L. Toohey-Kurth, Donna M. Mulrooney, Laura B. Goodman, Steve Bolin, Roger K. Maes, Beate Crossley, Mary Lea Killian, Janice C. Pedersen, Rebecca L. Tallmadge, Mangkey A. Bounpheng, Susanne Hinkley

Publikováno v: J Vet Diagn Invest
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, vol 32, iss 6

The exquisite sensitivity of in vitro amplification assays such as real-time polymerase chain reaction (rtPCR) requires the establishment of thorough and robust laboratory practices. To this end, an American Association of Veterinary Laboratory Diagn

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::79fd9e90b6194ebfad91d3c408b7860f
https://doi.org/10.1177/1040638720962076

Detection of the parasitic nematode, Pseudocapillaria tomentosa, in zebrafish tissues and environmental DNA in research aquaria

Autor: Donna M. Mulrooney, Michael L. Kent, Justin L. Sanders, Andree Hunkapiller, Noah Lawler, Lauren J Norris

Publikováno v: Journal of fish diseasesREFERENCES. 43(9)

Although zebrafish continue to increase in popularity as a vertebrate animal model for biomedical research, chronic infectious diseases in laboratory populations remain prevalent. The presence of pathogens such as Pseudocapillaria tomentosa, a parasi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c75a6e095ace49a831c526426e8c73ba
https://pubmed.ncbi.nlm.nih.gov/32720361

Natural cases of 2009 pandemic H1N1 Influenza A virus in pet ferrets

Autor: Sabrina L. Swenson, Leo Koster, Robin Weiss, Emilio DeBess, Donna M. Mulrooney, Annette Bredthauer, Melinda Jenkins-Moore, Mary Lea Killian, Rocky J. Baker, Judith A. Galeota

Publikováno v: Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. 22(5)

Respiratory swab samples were collected from 5 pet ferrets ( Mustela putorius furo) exhibiting influenza-like illness. The ferrets represented 3 households in 2 states. In each case, the owners reported influenza-like illness in themselves or family

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9cdaf7564cbf0aed1e9ea87aab21c239
https://pubmed.ncbi.nlm.nih.gov/20807944

Characteristics of a recent isolate of Sarcocystis neurona (SN7) from a horse and loss of pathogenicity of isolates SN6 and SN7 by passages in cell culture

Autor: Rocky J. Baker, S.J. Tornquist, Oliver C.H. Kwok, A. N. Hamir, Jitender P. Dubey, David S. Lindsay, D. E. Mattson, T. C. Gerros, C. A. Speer, Donna M. Mulrooney, Benjamin M. Rosenthal

Publikováno v: Veterinary parasitology. 95(2-4)

An isolate of Sarcocystis neurona (SN7) was obtained from the spinal cord of a horse with neurologic signs. The parasite was isolated in cultures of bovine monocytes and equine spleen cells. The organism divided by endopolygeny and completed at least

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9f3ede943ca35eba4c98606c289d5761
https://pubmed.ncbi.nlm.nih.gov/11223196

Seroprevalence of Toxoplasma gondii in llamas (Lama glama) in the northwest USA

Autor: Lora G. Rickard, Jitender P. Dubey, G.L. Zimmerman, Donna M. Mulrooney

Publikováno v: Veterinary parasitology. 44(3-4)

Serum samples from 283 llamas (Lama glama) from Oregon, Washington State and Idaho were tested for antibodies to Toxoplasma gondii using the modified agglutination test. Antibodies were found in 95 (33.5%) llamas. Percent seropositivity in serum dilu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c45c04d7ab4024741145ac2e6e1e90e5
https://pubmed.ncbi.nlm.nih.gov/1466137