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Publikováno v:
Analytical Biochemistry. 235:195-201
The tetracycline-controlled gene expression system utilizes the control elements of the tetracycline resistance operon encoded in TnlO of Escherichia coli to control gene expression in eukaryotic cells. Here we demonstrate the quantitative control of
Autor:
Dong X. Yin, Robert T. Schimke
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America. 93(8)
To study the effect of apoptosis on gene amplification, we have constructed HeLa S3 cell lines in which the expression of bcl-2 (BCL2) can be controlled by tetracycline in the growth medium. Induction of Bcl-2 expression caused a temporary delay of a