Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Donald O. Nwankwo"'
Autor:
Donald O. Nwankwo
Publikováno v:
Gene. 157:31-35
The respective genes (R-M) encoding restriction and modification systems from two Bacillus species which recognise the same nucleotide sequence, 5'-TCCGGA, have been cloned and expressed in Escherichia coli. The BspEI R-M genes were cloned on a 3.6-k
Autor:
Jack S. Benner, Phyllis A. Waite-Rees, Donald O. Nwankwo, Huimin Kong, Geoffrey G. Wilson, S. E. Roemer
Publikováno v:
Journal of Biological Chemistry. 269:683-690
The BcgI restriction enzyme from Bacillus coagulans is unusual in that it cleaves on both sides of its recognition site, CGAN6TGC, releasing a fragment that includes the site and several bases on each side. We report the organization and nucleotide s
Autor:
B Qiang, Barton E. Slatko, Laurie S. Moran, Donald G. Comb, Francine B. Perler, William E. Jack, Jack S. Benner, S K Hempstead, Donald O. Nwankwo, Rebecca Kucera
Publikováno v:
Proceedings of the National Academy of Sciences. 89:5577-5581
The DNA polymerase gene from the Archaea Thermococcus litoralis has been cloned and expressed in Escherichia coli. It is split by two intervening sequences (IVSs) that form one continuous open reading frame with the three polymerase exons. To our kno
Publikováno v:
Gene. 185(1)
The genes encoding the AatII restriction endonuclease and methylase from Acetobacter aceti have been cloned and expressed in Escherichia coli. The nucleotide sequences of aatIIM and aatIIR genes were determined. The aatIIM and aatIIR genes are 996 bp
Publikováno v:
Gene. 173(2)
The xmnIRM genes from Xanthomonas manihotis 7AS1 have been cloned and expressed in Escherichia coli. The nucleotide (nt) sequences of both genes were determined. The XmnI methyltransferase (MTase)-encoding gene is 1861 by in length and codes for 620
Autor:
Barton E. Slatko, Phyllis A. Waite-Rees, Jack S. Benner, Geoffrey G. Wilson, Lydia F. Dorner, Donald O. Nwankwo, Laurie S. Moran
Publikováno v:
Gene. 150(1)
The genes encoding the HindIII restriction endonuclease (R.HindIII ENase) and methyltransferase (M.HindIII MTase) from Haemophilus influenzae Rd were cloned and expressed in Escherichia coli and their nucleotide (nt) sequences were determined. The ge
Autor:
Donald O. Nwankwo, Daniel F. Heiter, Laura A. Sznyter, Tina Jager-Quinton, Barton E. Slatko, Karen R. Silber, Jack S. Benner, Geoffrey G. Wilson, Laurie S. Moran, Joan E. Brooks
Publikováno v:
Nucleic Acids Research. 17:979-997
BamHI, a Type II restriction modification system from Bacillus amyloliquefaciensH recognizes the sequence GGATCC. The methylase and endonuclease genes have been cloned into E. coli in separate steps; the clone is able to restrict unmodified phage. Al
Autor:
Keith D. Lunnen, Janet M. Barsomian, Russell R. Camp, Charles O. Card, Shu-Zi Chen, Rebecca Croft, Mary C. Looney, Marta M. Meda, Laurie S. Moran, Donald O. Nwankwo, Barton E. Slatko, Elizabeth M. Van Cott, Geoffrey G. Wilson
Publikováno v:
Gene. 74:25-32
We have cloned into Escherichia coli the genes for 38 type-II bacterial modification methyltransferases. The clones were isolated by selecting in vitro for protectively modified recombinants. Most of the clones modify their DNA fully but a substantia
Autor:
Sonia K. Guterman, Donald O. Nwankwo
Publikováno v:
European journal of biochemistry. 146(2)
Erwinia carotovora RNA polymerase consists of the holoenzyme structure sigma 2 beta beta' sigma as found in Escherichia coli and other bacteria. E. carotovora RNA polymerase can synthesize RNA using lambda, T7 of T4 DNA as templates; however, it is t
Cloning of two type II methylase genes that recognise asymmetric nucleotide sequences: FokI and HgaI
Autor:
Donald O. Nwankwo, Geoffrey G. Wilson
Publikováno v:
Moleculargeneral genetics : MGG. 209(3)
The modification genes of Flavobacterium okeanokoites and Haemophilus galinarum have been cloned into the vector pBR322 and expressed in Escherichia coli cells. FokI methylase gene is contained on a 3.80 kb piece of F. okeanokoites DNA. Plasmid const