Zobrazeno 1 - 10
of 100
pro vyhledávání: '"Donald M. Gray"'
Publikováno v:
Biopolymers. 99:484-496
The gene 5 protein (g5p) encoded by the Ff strains of Escherichia coli bacteriophages is a dimeric single-stranded DNA-binding protein (SSB) that consists of two identical OB-fold (oligonucleotide/oligosaccharide-binding) motifs. Ultrafast time-resol
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3b22cb29a7697a54c68435ea93fb9f60
https://doi.org/10.1002/bip.22204
https://doi.org/10.1002/bip.22204
Publikováno v:
Biochemistry. 50:8989-9001
Gene 5 protein (g5p) is a dimeric single-stranded DNA-binding protein encoded by Ff strains of Escherichia coli bacteriophages. The 2-fold rotationally symmetric binding sites of a g5p dimer each bind to four nucleotides, and the dimers bind with hig
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::383d68ce666826476b0e6c49d4eabb66
https://doi.org/10.1021/bi2006543
https://doi.org/10.1021/bi2006543
Publikováno v:
Protein Science. 11:601-613
The gene 5 protein (g5p) of the Ff virus contains five Tyr, individual mutants of which have now all been characterized by CD spectroscopy. The protein has a dominant tyrosyl 229-nm L(a) CD band that is shown to be approximately the sum of the five i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::521ab93e5a2c9a19a25e1de08bb168af
https://doi.org/10.1110/ps.30002
https://doi.org/10.1110/ps.30002
Publikováno v:
Biochemistry. 48:1099-1111
Noncanonical DNA structures such as G-quadruplexes might obstruct the binding of hRPA, compromising the accuracy of replication, and be a source of genomic instability. In this study, circular dichroism (CD) and electrophoretic mobility shift assay (
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b8a5f3561a44c94727f326405dd225e0
https://doi.org/10.1021/bi801538h
https://doi.org/10.1021/bi801538h
Publikováno v:
Biopolymers. 23:2639-2659
The first-neighbor approximation for the representation of double-stranded polynucleotide CD has been widely applied. Two apparently different formalisms have appeared. We discuss and compare those formalisms and show them to be identical. We present
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::035e8c1b72c96c110aa286b0303b6706
https://doi.org/10.1002/bip.360231133
https://doi.org/10.1002/bip.360231133
Publikováno v:
Nucleic Acids Research. 32:2008-2016
Antisense oligodeoxynucleotides (ODNs) have biological activity in treating various forms of cancer. The antisense effects of two types of 20mer ODNs, phosphorothioate-modified ODNs (S-ODNs) and S-ODNs with 12 2'-O-methyl groups (Me-S-ODNs), targeted
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::51b31d1eb0b8341b5a7bc42788bc034b
https://doi.org/10.1093/nar/gkh516
https://doi.org/10.1093/nar/gkh516
Autor:
Donald M. Gray, Jin-Der Wen
Publikováno v:
Biochemistry. 43:2622-2634
The gene 5 protein (g5p) encoded by filamentous Ff phages is an ssDNA-binding protein, which binds to and sequesters the nascent ssDNA phage genome in the process of phage morphogenesis. The g5p also binds with high affinity to DNA and RNA sequences
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::46b803d29074d9b291aee2689405187a
https://doi.org/10.1021/bi030177g
https://doi.org/10.1021/bi030177g
Publikováno v:
Journal of Biological Chemistry. 277:34208-34216
The assembly and stability of the RNA polymerase II transcription preinitiation complex on a eukaryotic core promoter involves the effects of TFIIA on the interaction between TATA-binding protein (TBP) and DNA. To extend our understanding of these in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3a223068626686a92de4634e80b271a6
https://doi.org/10.1074/jbc.m204808200
https://doi.org/10.1074/jbc.m204808200
Autor:
Donald M. Gray, Jin-Der Wen
Publikováno v:
Biochemistry. 41:11438-11448
The Ff gene 5 protein (g5p) is classified as a single-stranded DNA-binding protein. However, we previously showed that g5p binds with high affinity to a SELEX-selected G-rich 58-mer DNA oligomer, I-3, that forms an intramolecular G-quadruplex [Wen, J
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e0242452307f085d5b91ef2716a5bc5f
https://doi.org/10.1021/bi020276e
https://doi.org/10.1021/bi020276e
Autor:
Donald M. Gray, Tung-Chung Mou
Publikováno v:
Nucleic Acids Research. 30:749-758
One of the problems that hamper the use of antisense DNAs as effective drugs is the non-specific binding of chemically-modified oligonucleotides to cellular proteins. We previously showed that the affinity of a model ssDNA-binding protein, the Ff gen
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ca28ff6bf753b7dae891e672cb55bd7d
https://doi.org/10.1093/nar/30.3.749
https://doi.org/10.1093/nar/30.3.749