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pro vyhledávání: '"Donald J. Silvert"'
Autor:
Michael Snyder, Donald J. Silvert, Michael W. Hunkapiller, James W. Fristrom, David Yuen, Norman Davidson
Publikováno v:
Cell. 29:1027-1040
Most of a 9 kb region of the Drosophila genome containing genes for several cuticle proteins has been sequenced. Five cuticle-gene-like sequences have been identified and mapped. Amino acid sequences of four of the five major third instar cuticle pro
Autor:
James W. Fristrom, Donald J. Silvert
Publikováno v:
Insect Biochemistry. 10:341-355
In this review the current status of biochemical research on the development of imaginal discs is discussed, with particular emphasis on comparing and contrasting results from the Lepidoptera and Diptera. Acknowledging the considerable amount of evid
Publikováno v:
Developmental Biology. 89:379-388
Five stage-specific cuticles are produced during the development of Drosophila . Urea-soluble proteins were extracted from each developmental stage and compared by gel electrophoresis. Proteins from first and second instar cuticle are identical excep
Publikováno v:
Developmental biology. 91(2)
Mass-isolated imaginal discs of Drosophila melanogaster form a chitin-containing pupal procuticle In vitro. Optimal procuticle deposition occurs when the discs are incubated for 4–6 hr with 0.5–1.0 μg/ml of 20-hydroxyecdysone and then with less
Publikováno v:
Biochemistry. 23(24)
Proteins, soluble in 7 M urea, were extracted from third-instar larval and pupal cuticles of Drosophila melanogaster. Both extracts contain a limited number of polypeptides resolved by one- or two-dimensional electrophoresis. The five major larval pr