Výsledky vyhledávání - "Donald G. Comb"

Publikováno v: Nucleic acids research. 50(6)

The DNAs of bacterial viruses are known to contain diverse, chemically complex modifications to thymidine that protect them from the endonuclease-based defenses of their cellular hosts, but whose biosynthetic origins are enigmatic. Up to half of thym

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f91bd2463792eaf1d3c1bb41de2d68a4
https://pubmed.ncbi.nlm.nih.gov/34522950

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Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element

Autor: Shaorong Chong, Rebecca Kucera, Ming Qun Xu, Donald G. Comb, John J Pelletier, Luis M. Vence, David Landry, Fana B. Mersha, Jack S. Benner, Christine A. Hirvonen, Francine B. Perler, Melissa E. Scott, Henry Paulus

Publikováno v: Gene. 192:271-281

A novel protein purification system has been developed which enables purification of free recombinant proteins in a single chromatographic step. The system utilizes a modified protein splicing element (intein) from Saccharomyces cerevisiae (Sce VMA i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7558462856c54dad770817df53131240
https://doi.org/10.1016/s0378-1119(97)00105-4

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Activation of Glycosylasparaginase

Autor: Wei Liao, Jack S. Benner, T. Cui, Chudi Guan, Ching-Lun Lin, Vibha Rao, Donald G. Comb

Publikováno v: Journal of Biological Chemistry. 271:1732-1737

The activation mechanism of glycosylasparaginase of Flavobacterium meningosepticum has been analyzed by site-directed mutagenesis and activation of purified precursors in vitro. Mutation of Thr-152 to Ser or Cys leads to gene products that are not ac

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::03a0f4a304d385b03974c3a3d0a87077
https://doi.org/10.1074/jbc.271.3.1732

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Protein splicing: an analysis of the branched intermediate and its resolution by succinimide formation

Autor: H. Paulus, Yang Shao, Ming-Qun Xu, Francine B. Perler, Christopher J. Noren, Donald G. Comb

Publikováno v: The EMBO Journal. 13:5517-5522

Protein splicing involves the excision of an internal domain from a precursor protein and the ligation of the external domains so as to generate two new proteins. Study of this process has recently been facilitated by the isolation of a precursor and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0e091f4a8b8455c94a1f290530986984
https://doi.org/10.1002/j.1460-2075.1994.tb06888.x

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Intervening sequences in an Archaea DNA polymerase gene

Autor: B Qiang, Barton E. Slatko, Laurie S. Moran, Donald G. Comb, Francine B. Perler, William E. Jack, Jack S. Benner, S K Hempstead, Donald O. Nwankwo, Rebecca Kucera

Publikováno v: Proceedings of the National Academy of Sciences. 89:5577-5581

The DNA polymerase gene from the Archaea Thermococcus litoralis has been cloned and expressed in Escherichia coli. It is split by two intervening sequences (IVSs) that form one continuous open reading frame with the three polymerase exons. To our kno

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::517b89fd466aec87fc026485af903df2
https://doi.org/10.1073/pnas.89.12.5577

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Properties of the C-terminal domain of enzyme I of the Escherichia coli phosphotransferase system

Autor: Saul Roseman, Maurice W. Southworth, Kavita A. Vyas, Himatkumar V. Patel, Roshan L. Mattoo, Francine B. Perler, Donald G. Comb

Publikováno v: The Journal of biological chemistry. 281(26)

The bacterial phosphoenolpyruvate (PEP):glycose phosphotransferase system (PTS) mediates uptake/phosphorylation of sugars. The transport of all PTS sugars requires Enzyme I (EI) and a phosphocarrier histidine protein of the PTS (HPr). The PTS is stri

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7bc03ed5187580c956970dd0c948ea02
https://pubmed.ncbi.nlm.nih.gov/16547354

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