Zobrazeno 1 - 10
of 58
pro vyhledávání: '"Donal S. Luse"'
Autor:
Benjamin M. Spector, Mrutyunjaya Parida, Ming Li, Christopher B. Ball, Jeffery L. Meier, Donal S. Luse, David H. Price
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-17 (2022)
Here the authors digested chromatin with DNA fragmentation factor (DFF) prior to chromatin immunoprecipitation (DFF-ChIP) to depict transcription complex interactions with neighboring nucleosomes in cells. Applying this method to human cytomegaloviru
Externí odkaz:
https://doaj.org/article/33f9d6cf78be4231a55f0cb8c89e9986
Autor:
Mrutyunjaya Parida, Kyle A. Nilson, Ming Li, Christopher B. Ball, Harrison A. Fuchs, Christine K. Lawson, Donal S. Luse, Jeffery L. Meier, David H. Price
Publikováno v:
mBio, Vol 10, Iss 1 (2019)
ABSTRACT The large genome of human cytomegalovirus (HCMV) is transcribed by RNA polymerase II (Pol II). However, it is not known how closely this betaherpesvirus follows host transcriptional paradigms. We applied PRO-Seq and PRO-Cap methods to profil
Externí odkaz:
https://doaj.org/article/a5da4a0ae2b2409fbf8c0b9a8da26b4e
Publikováno v:
Nucleic acids research. 50(16)
The effects of rapid acute depletion of components of RNA polymerase II (Pol II) general transcription factors (GTFs) that are thought to be critical for formation of preinitiation complexes (PICs) and initiation in vitro were quantified in HAP1 cell
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b79977f13818f4803936a46589daa838
https://pubmed.ncbi.nlm.nih.gov/35947745
https://pubmed.ncbi.nlm.nih.gov/35947745
Autor:
Christopher B. Ball, Jeffrey Meier, David H. Price, Benjamin M Spector, Mrutyunjaya Parida, Donal S. Luse, Ming Li
Publikováno v:
Nature Communications. 13
Interactions of the RNA polymerase II (Pol II) preinitiation complex (PIC) and paused early elongation complexes with the first downstream (+1) nucleosome are thought to be functionally important. However, current methods are limited for investigatin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::045014d00712e0885f08ad7d2324aed6
https://doi.org/10.1038/s41467-022-29739-x
https://doi.org/10.1038/s41467-022-29739-x
Autor:
Elizabeth DeLaney, Donal S Luse
Publikováno v:
PLoS ONE, Vol 11, Iss 10, p e0163649 (2016)
Pausing during the earliest stage of transcript elongation by RNA polymerase II (Pol II) is a nearly universal control point in metazoan gene expression. The substoichiometric Pol II subunit Gdown1 facilitates promoter proximal pausing in vitro in ex
Externí odkaz:
https://doaj.org/article/f1c953618ce14f23921f7084cb829a0d
Publikováno v:
Nucleic Acids Research
To better understand human RNA polymerase II (Pol II) promoters in the context of promoter-proximal pausing and local chromatin organization, 5′ and 3′ ends of nascent capped transcripts and the locations of nearby nucleosomes were accurately ide
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::44df91a5598f43e494b73d8921ff82d4
https://doi.org/10.1093/nar/gkaa531
https://doi.org/10.1093/nar/gkaa531
Autor:
Elena Y. Kotova, Fu-Kai Hsieh, Han-Wen Chang, Natalia V. Maluchenko, Marie-France Langelier, John M. Pascal, Donal S. Luse, Alexey V. Feofanov, Vasily M. Studitsky
Publikováno v:
International Journal of Molecular Sciences; Volume 23; Issue 13; Pages: 7107
Human poly(ADP)-ribose polymerase-1 (PARP1) is a global regulator of various cellular processes, from DNA repair to gene expression. The underlying mechanism of PARP1 action during transcription remains unclear. Herein, we have studied the role of hu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cbdab4e21f292ffb6764166debcf67e3
https://doi.org/10.3390/ijms23137107
https://doi.org/10.3390/ijms23137107
Autor:
Donal S. Luse
Publikováno v:
Proceedings of the National Academy of Sciences. 116:22426-22428
A minimal set of general transcription factors (GTFs) is required for RNA polymerase II (pol II) to initiate transcription at promoters. For all eukaryotes, from yeast to mammals, the GTFs include TFIIA, TFIIB, TFIIE, TFIIF, TFIIH, and the TATA box b
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9ed43053ce88ad869f48fceae534ab57
https://doi.org/10.1073/pnas.1916582116
https://doi.org/10.1073/pnas.1916582116
Autor:
Geoffrey Collins, Ming Li, Donal S. Luse, Qiaolin Hu, David H. Price, Jeffery L. Meier, Christopher B. Ball
Publikováno v:
PLoS Pathogens, Vol 16, Iss 4, p e1008402 (2020)
PLoS Pathogens
PLoS Pathogens
Herpesvirus late promoters activate gene expression after viral DNA synthesis has begun. Alphaherpesviruses utilize a viral immediate-early protein to do this, whereas beta- and gammaherpesviruses primarily use a 6-member set of viral late-acting tra
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bdf7756d495fd51915b2c30b1d1fa09a
https://doi.org/10.1371/journal.ppat.1008402
https://doi.org/10.1371/journal.ppat.1008402
Autor:
Kyle A. Nilson, Jiannan Guo, John E. Brogie, David H. Price, Michael E. Turek, Elizabeth Delaney, Donal S. Luse
Publikováno v:
Molecular Cell. 59(4):576-587
The Cdk7 subunit of TFIIH phosphorylates RNA polymerase II (Pol II) during initiation, and, while recent studies show that inhibition of human Cdk7 negatively influences transcription, the mechanisms involved are unclear. Using in vitro transcription
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::52785496ff82dd9d2c4509aeaf3c2b14