Zobrazeno 1 - 10
of 195
pro vyhledávání: '"Dominic W S Wong"'
Autor:
Nagina Rafique, Saiqa Bashir, Muhammad Zubair Khan, Imran Hayat, Willium Orts, Dominic W S Wong
Publikováno v:
PLoS ONE, Vol 16, Iss 12, p e0256562 (2021)
Pectinolytic enzymes or pectinases are synthesized naturally by numerous microbes and plants. These enzymes degrade various kinds of pectin which exist as the major component of the cell wall in plants. A pectinase gene encoding endo-polygalacturonas
Externí odkaz:
https://doaj.org/article/d4fae8529d3d4493ad4e524c91da7e46
Publikováno v:
Advances in Enzyme Research. 11:1-10
Publikováno v:
BioResources, Vol 11, Iss 2, Pp 5204-5214 (2016)
A bacterial endo-polygalacturonase (endo-PGase) gene from the plant pathogen Pectobacterium carotovorum was cloned into pGAPZαA vector and constitutively expressed in Pichia pastoris. The recombinant endo-PGase secreted by the Pichia clone showed a
Externí odkaz:
https://doaj.org/article/b5b465f2084a40b0b816b6f725e220ae
Publikováno v:
Advances in Enzyme Research. :91-100
Publikováno v:
BioResources. 16:825-834
A feruloyl esterase (FAE) gene was isolated from rumen microbial metagenome that consisted of 774 bp encoding 258 amino acid residues. The gene was subcloned into pET 32b vector, expressed in Escherichia. coli, and the enzyme was purified in active f
Combinatorial Enzyme Approach for Production and Screening of Libraries of Feruloyl Oligosaccharides
Publikováno v:
Advances in Enzyme Research. :27-37
Combinatorial chemistry involves the chemical or biological synthesis of diverse variation of the structures of a target molecule and the library is then screened for variants of desirable target properties. The approach has been a focus of research
Autor:
Dominic W. S. Wong
Dieses klare und übersichtliche Buch bietet einen Einführungskurs in die Sprache des Genklonens und deckt dabei mikrobielle, pflanzliche und Säugetiersysteme ab. Es stellt die Grundlagen des Genklonens in einer gut organisierten und zugänglichen
Publikováno v:
Advances in Enzyme Research. :11-20
Combinatorial chemistry has been a focus of research activity in modern drug discovery and biotechnology. It is a concept by which a vast library of molecular diversity is synthesized and screened for target properties. This report is to illustrate t
Publikováno v:
MethodsX, Vol 5, Iss, Pp 669-675 (2018)
MethodsX
MethodsX
Graphical abstract
Direct cloning of metagenomes has proven to be a powerful tool for the exploration of the diverse sequence space of a microbial community leading to gene discovery and biocatalyst development. The key to such approach is the d
Direct cloning of metagenomes has proven to be a powerful tool for the exploration of the diverse sequence space of a microbial community leading to gene discovery and biocatalyst development. The key to such approach is the d
Publikováno v:
Applied microbiology and biotechnology. 103(20)
Feruloyl esterases (FAEs) are a key group of enzymes that hydrolyze ferulic acids ester-linked to plant polysaccharides. The cow's rumen is a highly evolved ecosystem of complex microbial microflora capable of converting fibrous substances to energy.