Zobrazeno 1 - 7
of 7
pro vyhledávání: '"Dominic J. Yee"'
Publikováno v:
Journal of the American Chemical Society. 130:14123-14128
We here present an optical method for monitoring the activity of the inducible aldo-keto reductases AKR1C2 and AKR1C3 in living human cells. The induction of these enzymes is regulated by the antioxidant response element (ARE), as demonstrated in rec
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::39da3f3e11b69cd7ffd7b8f6ba32cf78
https://doi.org/10.1021/ja801245y
https://doi.org/10.1021/ja801245y
Autor:
Vojtech Balsanek, Qingdong Zheng, Dalibor Sames, Paras N. Prasad, Steffen Jockusch, Nicholas J. Turro, Guang S. He, Dominic J. Yee, Haridas E. Pudavar, Marlin Halim
Publikováno v:
The Journal of Physical Chemistry C. 111:8872-8877
Fluorogenic probes for redox metabolism are shown to exhibit two-photon absorption using femtosecond pulses from a Ti/sapphire laser. The probes consist of a coumarin or naphthalene core and a ketone−alcohol functional group. The probe design is ba
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4c20d1317dd139b2fd7f2603a3447fce
https://doi.org/10.1021/jp067430h
https://doi.org/10.1021/jp067430h
Publikováno v:
Proceedings of the National Academy of Sciences. 103:13304-13309
The current arsenal of tools and methods for the continuous monitoring and imaging of redox metabolic pathways in the context of intact cells is limited. Fluorogenic substrates allow for direct measurement of enzyme activity in situ ; however, in con
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d42d942c7d1b796e13201d58c4ce5934
https://doi.org/10.1073/pnas.0604672103
https://doi.org/10.1073/pnas.0604672103
Publikováno v:
ACS chemical biology. 5(11)
The study of dynamic properties of metabolic and signaling networks is hindered by the lack of methods for imaging metabolic fluxes in individual intact cells. We describe a novel optical approach for measuring the changes of metabolic fluxes in cell
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e71b251f88e217270deebe3bc23720da
https://pubmed.ncbi.nlm.nih.gov/20863068
https://pubmed.ncbi.nlm.nih.gov/20863068
Publikováno v:
Journal of the American Chemical Society. 126(8)
A new fluorogenic substrate was developed for 3alpha-hydroxysteroid dehydrogenases (3alpha-HSD), including the human enzymes implicated in important physiological functions (androgen deactivation, neurosteroid activation). While ketone 5 is nonfluore
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4733f90f8d88301aa72a59ecd6299cf3
https://pubmed.ncbi.nlm.nih.gov/14982413
https://pubmed.ncbi.nlm.nih.gov/14982413
Autor:
Mary Ann Gawinowicz, Alexander Heim, Yelena Milgrom, Dalibor Sames, Doris Riether, Dominic J. Yee, Gong Chen
Publikováno v:
Journal of the American Chemical Society. 125(27)
We present the development of new affinity probes for protein labeling based on an epoxide reactive group. Systematic screening revealed that an epoxide functionality possesses the special combination of stability and reactivity which renders it stab
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9944fbc18b279433bbd9b2296061380e
https://pubmed.ncbi.nlm.nih.gov/12837082
https://pubmed.ncbi.nlm.nih.gov/12837082
Publikováno v:
Journal of the American Chemical Society. 127:4544-4545
This study describes the design of sensitive, selective, and fluorogenic reporter substrates for monoamine oxidase (MAO) enzymes. This was achieved by an iterative effort, guided by PET and TICT photophysical concepts, which led to the development of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c3460c9129617167a4e1ad2e2b5da3a4
https://doi.org/10.1021/ja0428457
https://doi.org/10.1021/ja0428457